Diagnosis and therapy with CRISPR advanced CRISPR based tools for point of care diagnostics and early therapies

Uppada, Vanita; Gokara, Mahesh; Rasineni, Girish Kumar

doi:10.1016/j.gene.2018.02.066

Cited by 36 publications

(41 citation statements)

References 87 publications

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“…Various properties of the CRISPR system have led to development of various diagnostic methods. While some tests make use of both the identification and cleavage of the target, other tests function based singularly on the guide RNA and Cas protein identification of the target [79]. Identification of the CRISPR system in pathogenic bacteria has become a useful diagnostic tool, due to the CRISPR being a part of most bacterial defense systems.…”

Section: Crispr As a Diagnostic Toolmentioning

confidence: 99%

“…Identification of the CRISPR system in pathogenic bacteria has become a useful diagnostic tool, due to the CRISPR being a part of most bacterial defense systems. Certain diagnostic methods based on these mechanisms are: serotyping/subtyping utilizing CRISPR, diagnostic assay based on single guide RNA (SgRNA), and another method of diagnostic assay based on CRISPR interference (dCas9) [79].…”

Section: Crispr As a Diagnostic Toolmentioning

confidence: 99%

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CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases

et al. 2020

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Interest in CRISPR technology, an instrumental component of prokaryotic adaptive immunity which enables prokaryotes to detect any foreign DNA and then destroy it, has gained popularity among members of the scientific community. This is due to CRISPR’s remarkable gene editing and cleaving abilities. While the application of CRISPR in human genome editing and diagnosis needs to be researched more fully, and any potential side effects or ambiguities resolved, CRISPR has already shown its capacity in an astonishing variety of applications related to genome editing and genetic engineering. One of its most currently relevant applications is in diagnosis of infectious and non-infectious diseases. Since its initial discovery, 6 types and 22 subtypes of CRISPR systems have been discovered and explored. Diagnostic CRISPR systems are most often derived from types II, V, and VI. Different types of CRISPR-Cas systems which have been identified in different microorganisms can target DNA (e.g. Cas9 and Cas12 enzymes) or RNA (e.g. Cas13 enzyme). Viral, bacterial, and non-infectious diseases such as cancer can all be diagnosed using the cleavage activity of CRISPR enzymes from the aforementioned types. Diagnostic tests using Cas12 and Cas13 enzymes have already been developed for detection of the emerging SARS-CoV-2 virus. Additionally, CRISPR diagnostic tests can be performed using simple reagents and paper-based lateral flow assays, which can potentially reduce laboratory and patient costs significantly. In this review, the classification of CRISPR-Cas systems as well as the basis of the CRISPR/Cas mechanisms of action will be presented. The application of these systems in medical diagnostics with emphasis on the diagnosis of COVID-19 will be discussed.

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Section: Crispr As a Diagnostic Toolmentioning

confidence: 99%

Section: Crispr As a Diagnostic Toolmentioning

confidence: 99%

CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases

et al. 2020

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“…[25][26][27] CRISPR-based technologies spread further into the area of molecular diagnostics and may replace PCR in many applications in the near future. [28][29][30][31] (Table 1) In 2016, CRISPR-Cas systems were first developed to identify nucleic acids for molecular diagnostics. [32] Demand for instrument-free nucleic acid detection technologies has driven the development of multiple techniques for isothermal amplification.…”

Section: Detection Of Nucleic Acids By Crispr-casmentioning

confidence: 99%

<strong>SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases and Cancer-Associated Mutations</strong>

Mustafa¹,

Makhawi²

2020

Preprint

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Sensitive and precise nucleic acid detection is critical for clinical diagnostics and biotechnological advancements. Diagnostic in infectious disease field is very unique from diagnosing any other disease, that is time is of the essence; in outbreaks people die even with each passing hour in some cases, if the correct diagnosis wasn't make; for example Zika in particularly is a very challenging virus to diagnose, because it's in very few numbers of copies in the infected person, so it need high sensitive diagnostic approach to spot it, In particular, the advanced tools SHERLOCKv2 and DETECTR, give almost an immediate detection of attomolar amounts of pathogenic nucleic acids with specificity similar to that of PCR but with slight technical settings and that will guide the correct intervention for the patient. SHERLOCKv2 and DETECTR technologies are game changers for our ability to identify infectious disease and rapid detection of tumor DNA or cancer-related viruses with ultra-sensitive tests that don’t require a lot of complicated processing to go through. In this paper, we will review cutting-edge infectious disease diagnosis by CRISPR-Cas systems.

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“…The study of bacteriophages continues to yield remarkably useful and exciting insights and new tools. Recently this includes tools derived from the bacterial immune system, or CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), which act against phages and plasmids (Uppada et al 2018). These tools are now being used extensively for editing the genomes of many plants and animals, including, potentially, humans and companion animals (Lee et al 2018).…”

Section: What Are Bacteriophages?mentioning

confidence: 99%

Bacteriophage therapy for management of bacterial infections in veterinary practice: what was once old is new again

Squires

2018

New Zealand Veterinary Journal

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Bacteriophages (or phages) are naturally-occurring viruses that can infect and kill bacteria. They are remarkably diverse, numerous and widespread. Each phage has a narrow host range yet a large majority of bacteria studied so far play host to bacteriophages, hence the remarkable phage diversity. Phages were discovered just over 100 years ago and they have been used for treatment of bacterial infections in humans and other animals since the 1920s. They have also been studied intensively and this has led to, and continues to lead to, major insights in the fields of molecular biology and recombinant DNA technology, including that DNA is the genetic material, nucleotides are arranged in triplets to make codons, and messenger RNA is needed for protein synthesis. This article begins with a description of bacteriophages and explains why there has recently been a strong resurgence of interest in their clinical use for treatment of bacterial infections, particularly those caused by organisms resistant to multiple antimicrobial compounds. The history of bacteriophage therapy is briefly reviewed, followed by a review and critique of promising but very limited clinical research on the use of bacteriophages to treat bacterial infections in dogs. Other potential veterinary uses and benefits of bacteriophage therapy are also briefly discussed. There are important practical challenges that will have to be overcome before widespread implementation and commercialisation of bacteriophage therapy can be achieved, which are also considered.

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Diagnosis and therapy with CRISPR advanced CRISPR based tools for point of care diagnostics and early therapies

Cited by 36 publications

References 87 publications

CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases

CRISPR-Based Diagnosis of Infectious and Noninfectious Diseases

<strong>SHERLOCK and DETECTR: CRISPR-Cas Systems as Potential Rapid Diagnostic Tools for Emerging Infectious Diseases and Cancer-Associated Mutations</strong>

Bacteriophage therapy for management of bacterial infections in veterinary practice: what was once old is new again

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