Diagnosis and molecular phylogeny of rabies virus obtained from an Indian wolf

Reddy, G.B. Manjunatha; Sumana, K.; Shankar, B. P.; Patil, Supriya; Rahman, Muhammad Habibur

doi:10.5958/0973-970x.2015.00072.3

Cited by 1 publication

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The phylogenetic analysis of the N gene revealed that all the isolates included in the study are clustered into a single lineage /arctic-like cluster, reflecting the conserved nature of the N gene among the RV strains. Similar observations about N-gene phylogenetic analysis were recorded by [29, 30, 7, 31]. Three isolates R28, R8 and R7 in the present study showed a close relationship with a previous isolate from Mumbai [KF535208.1 (RAB 20)].…”

Section: Resultssupporting

confidence: 91%

“…The isolates under study were grouped under lineage 1. A similar pattern was observed by Cherian et al [12], [30] and Singh et al [39] about the Arctic lineages of RV circulating in other parts of India. Phylogenetic findings under the present study showed concordance with previous studies and indicated that RV isolates are in one cluster based on geographical regions [40] and [41].…”

Section: Migration Pattern Of Rvsupporting

confidence: 88%

Section: Resultsmentioning

confidence: 81%

“…A similar pattern was observed by Cherian et al . [12], [30] and Singh et al . [39] about the Arctic lineages of RV circulating in other parts of India.…”

Section: Resultsmentioning

confidence: 99%

“…The sequences from Bangladesh and Afghanistan were close to this cluster as compared to those from the USA and China. These reiterate that RV spread is a localized phenomenon [30]. He et al [34] used neighbour-net split tree to determine potential recombination events to identify novel RV lineages in China and reported that RV strains J and CQ92 were a mosaic of two Chinese lineages.…”

Section: Phylogenetic Analysis Of the Partial N And G Genementioning

confidence: 99%

See 4 more Smart Citations

Evolutionary analysis of rabies virus using the partial Nucleoprotein and Glycoprotein gene in Mumbai region of India

Pharande

Majee

Gaikwad³

et al. 2021

Journal of General Virology

View full text Add to dashboard Cite

Nearly 1.7 million cases of dog bites are reported every year in India and many cases of animal rabies are left unattended and undiagnosed. Therefore, a mere diagnosis of rabies is not sufficient to understand the epidemiology and the spread of the rabies virus (RV) in animals. There is a paucity of information about the evolutionary dynamics of RV in dogs and its biodiversity patterns in India. In total, 50 dog-brain samples suspected of rabies were screened by the nucleoprotein- (N) and glycoprotein- (G) gene PCR. The N and G genes were subsequently sequenced to understand the molecular evolution in these genes. The phylogenetic analysis of the N gene revealed that six isolates in the Mumbai region belonged to a single Arctic lineage. Time-scaled phylogeny by Bayesian coalescent analysis of the partial N gene revealed that the time to the most recent common ancestor (TMRCA) for the sequences belonged to the cluster from 2006.68 with a highest posterior density of 95 % betweeen 2005–2008, which is assigned to Indian lineage I. Migration pattern revealed a strong Bayes factor between Mumbai to Delhi, Panji to Hyderabad, Delhi to Chennai, and Chennai to Chandigarh. Phylogenetic analysis of the G gene revealed that the RVs circulating in the Mumbai region are divided into three lineages. Time-scaled phylogeny by the Bayesian coalescent analysis method estimated that the TMRCA for sequences under study was from 1993 and Indian clusters was from 1962. In conclusion, the phylogenetic analysis of the N gene revealed that six isolates belonged to single Arctic lineages along with other Indian isolates and they were clustered into a single lineage but divided into three clades based on the G-gene sequences. The present study highlights and enhances the current molecular epidemiology and evolution of RV and revealed strong location bias and geographical clustering within Indian isolates on the basis of N and G genes.

show abstract

Section: Resultssupporting

confidence: 91%

Section: Migration Pattern Of Rvsupporting

confidence: 88%

Section: Resultsmentioning

confidence: 81%

“…A similar pattern was observed by Cherian et al . [12], [30] and Singh et al . [39] about the Arctic lineages of RV circulating in other parts of India.…”

Section: Resultsmentioning

confidence: 99%

Section: Phylogenetic Analysis Of the Partial N And G Genementioning

confidence: 99%

See 3 more Smart Citations