Diagnosis and follow-up of Bartonella henselae infection in the spleen of an immunocompetent patient by real-time quantitative PCR

Liberto, Maria Carla; Matera, Giovanni; Lamberti, Angelo Giuseppe; Quirino, Angela; Barreca, Giorgio Settimo; Marascio, Nadia; Baudi, Francesco; Caroleo, Benedetto; Staltari, Orietta; Focà, Alfredo

doi:10.1099/jmm.0.050617-0

Cited by 8 publications

(5 citation statements)

References 18 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…31 , 34 Herein, we describe 3 new cases of HS-CSD and review 33 previously reported cases with a focus on clinical descriptions, diagnosis, and therapeutic approaches. 4 , 6 , 9 , 10 , 18 , 23 , 24 , 26 , 27 , 29 , 30 , 33 , 35 , 36 , 38 – 42 , 44 , 45 …”

Section: Introductionmentioning

confidence: 99%

Hepatosplenic Cat Scratch Disease in Immunocompetent Adults

et al. 2014

View full text Add to dashboard Cite

Cat-scratch disease (CSD) is the most frequent presentation of Bartonella henselae infection. It has a worldwide distribution and is associated with a previous history of scratch or bite from a cat or dog. CSD affects children and teenagers more often (80%) than adults, and it usually has a self-limiting clinical course. Atypical clinical course or systemic symptoms are described in 5%–20% of patients. Among them, hepatosplenic (HS) forms (abscess) have been described. The majority of published cases have affected children or immunosuppressed patients. Few cases of HS forms of CSD in immunocompetent adult hosts have been reported, and data about the management of this condition are scarce. Herein, we present 3 new cases of HS forms of CSD in immunocompetent adults and review 33 other cases retrieved from the literature. We propose an approach to clinical diagnosis and treatment with oral azithromycin.

show abstract

Section: Introductionmentioning

confidence: 99%

Hepatosplenic Cat Scratch Disease in Immunocompetent Adults

et al. 2014

View full text Add to dashboard Cite

show abstract

“…PCR ND. Serology positive ND 2 weeks/NA Ciprofloxacine 2 week, CT-guided drainage, splenectomy Case 11 , 2013 [ 11 ] 34 F Abdominal pain, fever Multiple spleen abscess, skin lesion, lymphade NO WBC normal, CRP, and liver enzymes NA Abdomen MRI PCR and serology positive. Culture ND ND NA/2.5 months AZT+DOX 5 d, CLR+RIF 5 weeks Case 12 [ 12 ], 2012 36 F Abdominal pain, fever 5 cm right hepatic lobe mass NO WBC normal, CRP NA, liver enzymes elevated Abdomen CT Culture positive.…”

Section: Methodsmentioning

confidence: 99%

Hepatosplenic Cat Scratch Disease: Description of Two Cases Undergoing Contrast-Enhanced Ultrasound for Diagnosis and Follow-Up and Systematic Literature Review

Tirotta

Mazzeo

Nizzoli

2021

SN Compr. Clin. Med.

View full text Add to dashboard Cite

Cat scratch disease (CSD) is a disease usually characterized by self-limited lymphadenopathy of the young man. Rarely CSD, however, can manifest itself as an unusual hepatosplenic form (HS-CSD) in immunocompetent patients. HS-CSD diagnosis is generally based on clinical features, imaging, and serologies, but sensitivity of serologies is very variable, like that of other diagnostic methods, as Warthin-Starry silver stain and isthology. Also there are no specific markers for the follow-up. The use of the CEUS (abdominal contrast-enhanced ultrasound) in HS-CSD is not previously described in literature examined, but we think that CEUS can be of help to diagnosis and follow-up of these patients, even after an initial CT scan, because it is a sensitive method, as seen in other diseases associated with granulomas, such as sarcoidosis. We describe 2 new cases of HS-CSD, and we performed a systematic review of the clinical cases reported in the past 10 years in the literature associated to an analysis of clinical, diagnostic, and therapeutic aspects of the disease.

show abstract

“…A homemade real-time PCR with SYBR Green 1 dye was performed as previously described [ 3 , 4 ]. Briefly, DNA extraction from whole blood was carried out using the QIAamp DNA Blood Mini Kit (QIAGEN ® Group) according to the manufacturer’s instruction.…”

Section: Methodsmentioning

confidence: 99%

Utility of Molecular Identification and Quantitation of Bartonella Species with Species-Specific Real-Time PCR for Monitoring Treatment Response: A Case Series

Mazzitelli¹,

Lamberti²,

Quirino³

et al. 2018

TOMICROJ

Self Cite

View full text Add to dashboard Cite

Background:Bartonella species are intracellular bacteria capable of producing several diseases in humans. The three most common and wellknown diseases are cat scratch disease (CSD), caused by B. henselae, trench fever, caused by B. quintana and Carrion’s Disease, caused by B. bacilliformis. Signs and symptoms are very different and aspecific: Fatigue, fever, headache, lymphadenopathy, malaise, loss of weight. No data exist to support guidelines’ recommendations to decide which drugs should be optimally used and how long they should be administered. Therefore, a marker of treatment response is needed to guide treatment strategies.Methods:We report herein three cases in which a species specific Reverse-Transcriptase Polymerase-Chain-Reaction (RT PCR) developed in-house was performed and compared to serology in order to make diagnosis and to evaluate treatment response.Results:Our species-specific RT PCR seemed to play a fundamental role both in diagnosis and treatment. Moreover, a discrepancy with the serology results was found.Conclusion:Further studies are necessary to validate these results and elucidate what is the best treatment for this pleomorphic disease. However, in absence of clear guidelines, RT PCR may be useful to orientate kind of treatment ad its duration.

show abstract

Diagnosis and follow-up of Bartonella henselae infection in the spleen of an immunocompetent patient by real-time quantitative PCR

Cited by 8 publications

References 18 publications

Hepatosplenic Cat Scratch Disease in Immunocompetent Adults

Hepatosplenic Cat Scratch Disease in Immunocompetent Adults

Hepatosplenic Cat Scratch Disease: Description of Two Cases Undergoing Contrast-Enhanced Ultrasound for Diagnosis and Follow-Up and Systematic Literature Review

Utility of Molecular Identification and Quantitation of Bartonella Species with Species-Specific Real-Time PCR for Monitoring Treatment Response: A Case Series

Contact Info

Product

Resources

About