Diagnosing the drug resistance signature in Plasmodium falciparum: a review from contemporary methods to novel approaches

Murmu, Laxman Kumar; Sahu, Arpita Arsmika; Barik, Tapan Kumar

doi:10.1007/s12639-020-01333-2

Cited by 6 publications

(2 citation statements)

References 61 publications

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“…Despite a declining trend of malaria incidences over the past decade (Rosenthal et al, 2019), pregnant women and children under five still bear the highest burden. P. falciparum, the deadliest among the Plasmodium spp (Murmu et al, 2021), has a complex life cycle alternating between human and mosquito vectors. This, coupled with a lack of effective vaccines, parasite resistance to the available antimalarials, and mosquito resistance to insecticides, hinder efforts to prevent, control and eliminate malaria (Dimala et al, 2018;Cowell and Winzeler, 2019;Maier et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Genetic variation of the Plasmodium falciparum circumsporozoite protein in parasite isolates from Homabay County in Kenya

Maina,

Musundi,

Kuja

et al. 2024

Front. Parasitol.

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The Plasmodium falciparum Circumsporozoite Protein (PfCSP) has been used in developing the RTS,S, and R21 malaria vaccines. However, genetic polymorphisms within Pfcsp compromise the effectiveness of the vaccine. Thus, it is essential to continuously assess the genetic diversity of Pfcsp, especially when deploying it across different geographical regions. In this study, we assessed the genetic diversity of the Pfcsp on isolates from Homabay County, a malaria-endemic region in western Kenya, and compared it against other isolates from Kenya. We extracted DNA from 27 microscopically confirmed P. falciparum positive samples and conducted Illumina sequencing to generate paired-end short reads. The sequences were then mapped to the Pf3D7 reference genome, and genetic variation was analyzed using bcftools. Additionally, we retrieved isolates from two other malaria-endemic regions in Kenya, Kisumu (n=58) and Kilifi (n=596), from MalariaGEN version 7 and compared their genetic diversity and natural selection. We also evaluated the predicted binding affinities for HLA class I and II supertype alleles for the identified haplotypes using NetMHCpan and NetMHCIIpan. Our results show that the N-terminal of PfCSP was relatively conserved with a notable mutation at A98G across all isolates. The number of NANP repeats varied across the three Kenyan sites within the central repeat region. Furthermore, the C-terminal region showed polymorphism within the Th2R and Th3R regions. Haplotype network analysis of the Kenyan isolates revealed 69 haplotypes, with the 3D7 reference being found in the most prevalent haplotype. When assessing the predicted binding affinities between supertypes in HLA class I and II with the identified haplotypes, we observed stronger predicted binding affinities to multiple haplotypes except for those containing the 3D7 reference. The results suggest the need to take into account the existing changes occurring in Pfcsp while developing malaria vaccines.

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Section: Introductionmentioning

confidence: 99%

Genetic variation of the Plasmodium falciparum circumsporozoite protein in parasite isolates from Homabay County in Kenya

Maina,

Musundi,

Kuja

et al. 2024

Front. Parasitol.

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show abstract

“…on the evolution, propagation, and mechanisms of drug resistance in malaria parasites that infect humans, whose thorough account can be assessed elsewhere in recent reviews [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

On the contribution of the rodent model Plasmodium chabaudi for understanding the genetics of drug resistance in malaria

Cravo

2022

Parasitology International

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Precision Medicine in Infectious Disease

Alvarez

Hasanzad

Meybodi

et al. 2022

Precision Medicine in Clinical Practice

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Diagnosing the drug resistance signature in Plasmodium falciparum: a review from contemporary methods to novel approaches

Cited by 6 publications

References 61 publications

Genetic variation of the Plasmodium falciparum circumsporozoite protein in parasite isolates from Homabay County in Kenya

Genetic variation of the Plasmodium falciparum circumsporozoite protein in parasite isolates from Homabay County in Kenya

On the contribution of the rodent model Plasmodium chabaudi for understanding the genetics of drug resistance in malaria

Precision Medicine in Infectious Disease

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