Diagnosing schistosomiasis-induced liver morbidity: implications for global control

Olveda, David U.; Inobaya, Marianette T.; Olveda, Remigio M.; Vinluan, Marilyn L.; Ng, Shu‐Kay; Weerakoon, Kosala; McManus, Donald P.; Ramm, Grant A.; Harn, Donald A.; Li, Yuesheng; Lam, Alfred King‐Yin; Guevarra, Jerric R.; Ross, Allen G.

doi:10.1016/j.ijid.2016.10.024

Cited by 21 publications

(29 citation statements)

References 31 publications

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“…Detection of an individual miRNA provided only moderate diagnostic value for staging schistosomal hepatic fibrosis (AUC from 0.6270 to 0.6838); although similar to miR-150-5p, the best diagnostic performance was obtained with the combination of miR-150-5p, let-7a-5p and miR-146a-5p (AUC: 0.6997) ( Table 3 ). These values are comparable with the ELF test (AUC: 0.68) and HA serum levels (AUC: 0.69) for differentiating grade II-III fibrosis from grade 0-I fibrosis for the same human cohort used here [ 37 ]. And the ELF test has been previously considered superior to simple panels (such as Fibrosis-4 and aspartate aminotransferase to platelet ratio) in detecting advanced fibrosis [ 37 ].…”

Section: Discussionsupporting

confidence: 72%

“…The human cohort study was carried out in 18 schistosomiasis-endemic barangays in the municipalities of Laoang and Palapag, Northern Samar, the Philippines. Detailed information of the study population is described elsewhere as part of a hepatic morbidity study [ 37 , 39 ]. We enrolled 163 individuals (all negative for HBV/HCV infection) from the cohort all of whom were assessed for liver fibrosis severity by ultrasound scan using a portable gray-scale ultrasonogram equipped with 3 MHz curve array transducer (SONOACE X1; Madison Co., Ltd., Seoul, South Korea).…”

Section: Methodsmentioning

confidence: 99%

“…Hepatic fibrosis assessment was adopted from the practical standards established for ultrasonography by the WHO/TDR in 2000 (WHO Special Programme for Research and Training in Tropical Diseases). Ultrasound findings were determined by consensus agreement of two experienced ultrasonographers [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

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Circulating miRNAs as footprints for liver fibrosis grading in schistosomiasis

Cai

Olveda

et al. 2018

EBioMedicine

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BackgroundChronic infection with Schistosoma japonicum or S. mansoni results in hepatic fibrosis of the human host. Staging fibrosis is crucial for the prognosis and to determine the rapid need of treatment in patients with schistosomiasis.MethodsTo establish whether there is a correlation between circulating microRNA (miRNA) level and fibrosis progression in schistosomiasis, ten miRNAs were selected to assess their potential in grading schistosomiasis liver fibrosis. This was done firstly in two mouse strains (C57BL/6 and BALB/c) to determine the temporal expression profiles in serum over the course of S. japonicum infection, and then within a cohort of 163 schistosomiasis japonica patients with different grades of liver fibrosis.FindingFour miRNAs (miR-150-5p, let-7a-5p, let-7d-5p and miR-146a-5p) were able to distinguish patients with mild versus severe fibrosis. The level of serum miR-150-5p showed the most promising potential for grading hepatic fibrosis in schistosomiasis. The diagnostic performance of miR-150-5p in discriminating mild from severe fibrosis is comparable with that of the ELF test and serum HA level. In addition, the serum levels of the four miRNAs rebounded in infected C57BL/6 mice, after 6 months post treatment, following the regression of liver fibrosis, thereby providing further support for the utility of these miRNAs in grading schistosomal hepatic fibrosis.Interpretation.Circulating miRNAs can be a supplementary tool for assessing hepatic fibrosis in human schistosomiasis.Fund (NHMRC) of Australia (APP1102926, APP1037304 and APP1098244).

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Section: Discussionsupporting

confidence: 72%

Section: Methodsmentioning

confidence: 99%

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Circulating miRNAs as footprints for liver fibrosis grading in schistosomiasis

Cai

Olveda

et al. 2018

EBioMedicine

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“…The second serum batch ( n = 412) were obtained from the same endemic area in 2015, two years after a program of mass drug (i.e., 40 mg/kg praziquantel) administration. Of these, 108 and 304 patients were confirmed as positive and negative infection, respectively, using the Kato-Katz method ( Olveda et al, 2017 ). Serum samples of healthy individuals were obtained from Heilongjiang Province, a non-endemic area for schistosomiasis in China.…”

Section: Methodsmentioning

confidence: 99%

A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines

Cai

Weerakoon

et al. 2017

EBioMedicine

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Schistosoma japonicum is stubbornly persistent in China and the Philippines. Fast and accurate diagnostic tools are required to monitor effective control measures against schistosomiasis japonica. Promising antigen candidates for the serological diagnosis of schistosomiasis japonica have generally been identified from the Chinese strain of S. japonicum. However, the Chinese (SjC) and Philippine (SjP) strains of S. japonicum express a number of clear phenotypic differences, including aspects of host immune responses. This feature thereby emphasized the requirement to determine whether antigens identified as having diagnostic value for SjC infection are also suitable for the diagnosis of SjP infection. In the current study, 10 antigens were selected for comparison of diagnostic performance of the SjP infection using ELISA. On testing of sera from 180 subjects in the Philippines, SjSAP4 exhibited the best diagnostic performance with 94.03% sensitivity and 98.33% specificity using an optimized serum dilution. In another large scale testing with 412 serum samples, a combination (SjSAP4 + Sj23-LHD (large hydrophilic domain)) provided the best diagnostic outcome with 87.04% sensitivity and 96.67% specificity. This combination could be used in future for serological diagnosis of schistosomiasis in the Philippines, thereby representing an important component for monitoring integrated control measures.

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“…ed., 13 besides scientific articles that are duly referenced. [14][15][16][17][18][19][20][21][22][23][24][25][26] We analyzed the death certificate lines and their clinical correlation in order to identify if schistosomiasis would be the basic cause of death, based on the disease physiopathology.…”

Section: Consulted Bibliographies For Analysis Of the Death Basic Causesmentioning

confidence: 99%

Schistosoma mansoni in Brazil in 2015: retrieval of deaths and characteristics of patients that evolved to death

2019

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Introduction: Schistosomiasis is a disease endemic in Brazil. We analyzed the death records from the Brazilian Mortality Information System with the aim of retrieving deaths due to schistosomiasis and describing the patients that evolved to death in Brazil in the year of 2015. Methods: We evaluated the death certificates for retrieving basic causes due to schistosomiasis, estimated the mortality rate and described the sociodemographic characteristics of patients. Result: To retrieve the basic cause, we analyzed 150 death certificates and we found 39 (26%). Of the 406 studied deaths, 111 (27.3%) occurred in people aged between 60 and 69 and 101 (24.9%) between 70 and 79. Regarding the educational level, 196 (62.4%) had not concluded the elementary school and 85 (27.1%) had never studied. As to race/skin color, 248 deaths (65.1%) occurred in black or pardo. Pernambuco registered more deaths, with 119 (29.3%). Alagoas was the state presenting the highest mortality rate, with 1.62/100,000 residents. As immediate causes of death, most of them, 118 (29.1%), occurred by hypovolemic shock. Conclusions: Deaths were more frequent in older ages, consistent with the disease chronic evolution, in people with lower educational level, and in subjects that reported themselves as black or pardo.

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Diagnosing schistosomiasis-induced liver morbidity: implications for global control

Cited by 21 publications

References 31 publications

Circulating miRNAs as footprints for liver fibrosis grading in schistosomiasis

Circulating miRNAs as footprints for liver fibrosis grading in schistosomiasis

A Parallel Comparison of Antigen Candidates for Development of an Optimized Serological Diagnosis of Schistosomiasis Japonica in the Philippines

Schistosoma mansoni in Brazil in 2015: retrieval of deaths and characteristics of patients that evolved to death

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