Diagnosing Helicobacter pylori In Vivo by Confocal Laser Endoscopy

Kießlich, Ralf; Goetz, Martin; Burg, Juergen; Stolte, Manfred; Siegel, Ekkehard; Maeurer, Markus; Thomas, Steven; Strand, Dennis; Galle, Peter R.; Neurath, Markus F.

doi:10.1053/j.gastro.2004.12.035

Cited by 152 publications

(101 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, it may be helpful to characterise airway inflammation in chronic diseases such as asthma and chronic obstructive pulmonary disease, with a possible influence on therapy strategies. Finally, CLE may open the door to molecular imaging of airway epithelial cells in vivo, as this technique has been recently used beyond acriflavine staining for vascular endothelial growth factor receptor and CD44v6 staining of colonic epithelial cells [23][24][25][26][27].…”

Section: Lung Cancer Fs Fuchs Et Almentioning

confidence: 99%

Confocal laser endomicroscopy for diagnosing lung cancerin vivo

et al. 2012

Self Cite

View full text Add to dashboard Cite

Confocal laser endomicroscopy is a novel endoscopic technique that may allow imaging of living cells in lung tissue in vivo. We assessed the potential of this technique for the detection of histology during screening bronchoscopy for lung cancer.32 patients with suspected malignancies underwent bronchoscopy with endomicroscopy using acriflavine hydrochloride. Standardised areas and localised lesions were analysed by in vivo confocal imaging during bronchoscopy and biopsies were taken. Confocal images were graded and correlated prospectively with conventional histology from biopsies.Acriflavine hydrochloride yielded high-quality confocal images and strongly labelled airway epithelial cells. No side-effects were noted. 75 522 confocal images from 56 different locations were compared prospectively with histological data from biopsy specimens. Endomicroscopy allowed subsurface imaging with detailed analysis of cellular and subcellular structures. Neoplastic changes could be predicted with high accuracy (sensitivity 96.0%, specificity 87.1%, accuracy 91.0%).Confocal laser endomicroscopy with acriflavine is a novel diagnostic tool for the analysis of living cells during bronchoscopy and permits virtual histology of neoplastic changes in the airways with high accuracy. This technique may enable the rapid diagnosis of neoplasia during ongoing endoscopy in patients with suspected lung cancer.

show abstract

Section: Lung Cancer Fs Fuchs Et Almentioning

confidence: 99%

Confocal laser endomicroscopy for diagnosing lung cancerin vivo

et al. 2012

Self Cite

View full text Add to dashboard Cite

show abstract

“…Such gaps coincide with increased bacterial translocation in patients with inflammatory bowel diseases. Similar to Helicobacter pylori in the stomach [35], bacteria were also identified using fluorescein in the human colon as tiny white dots within the lamina propria and were confirmed by staining of microscopically targeted biopsies. Intramucosal bacteria were significantly more frequent in patients with inflammatory bowel diseases than in healthy controls [97].…”

Section: Functional Imagingmentioning

confidence: 73%

“…Here, CLE is able to guide future management by taking multiple optical biopsies within few seconds and screen larger areas of the mucosal surface. H. pylori was detected in gastritis using topical staining with acriflavine in a proof-of-principle paper [35] after demonstrating that H. pylori actively incorporates acriflavine in culture. Although H. pylori-diagnosis is standardized and reliably available using established methods, this exemplifies the high resolution of eCLE and was the first example of an in vivo microscopic technique to visualize live bacteria during ongoing endoscopy.…”

Section: Endomicroscopy Of Gastricmentioning

confidence: 99%

Confocal Laser Endomicroscopy: Applications in Clinical and Translational Science—A Comprehensive Review

Goetz

2012

ISRN Pathology

Self Cite

View full text Add to dashboard Cite

Confocal laser endomicroscopy (CLE) is a novel tool in the endoscopist's armamentarium. It allows on-site histological information. The ability of gastroenterologists to interpret such microscopic information has been demonstrated in multiple studies from the upper and lower gastrointestinal tract. Recently, the field of application has expanded to provide hepatobiliary and intra-abdominal CLE imaging. CLE allows "smart," targeted biopsies and is able to guide endoscopic interventions. But CLE is also translational in its approach and permits functional imaging that significantly impacts on our understanding of gastrointestinal diseases. Molecular imaging with CLE allows detection and characterization of lesions and may even be used for prediction of response to targeted therapy. This paper provides a comprehensive review over current applications of CLE in clinical applications and translational science.

show abstract

“…Acriflavine not only stains the gastric epithelial cells but also selectively stains HP which is seen as multiple white focal lesions at the surface and in deeper layers of the gastric epithelium. 39 In a study of 118 consecutive patients with H. pylori infection-associated gastritis the sensitivity and specificity of CLE was 83% and 91% for the diagnosis of H. pylori infection, 93% and 95% for predicting glandular atrophy and 99% and 100% for diagnosing intestinal metaplasia, respectively. 40 …”

Section: Helicobacter Pylorimentioning

confidence: 99%

Status of confocal laser endomicroscopy in gastrointestinal disease

Humphris

Swartz

Egan

et al. 2012

View full text Add to dashboard Cite

Confocal laser endomicroscopy (CLE) is an advanced imaging technique which combines conventional white light endoscopy (WLE) with an integrated or probe based confocal microscope. This allows microscopic examination of the surface epithelium and in vivo diagnosis during endoscopy. Established CLE applications include the diagnosis of Barrett's oesophagus, gastric intestinal metaplasia, coeliac disease and microscopic colitis. CLE can differentiate hyperplastic from adenomatous polyps in the colon and may obviate the need to biopsy all polyps at endoscopy. CLE is particularly helpful in surveillance endoscopy in inflammatory bowel disease where it has been shown to reduce the number of biopsies required and improve the detection of dysplasia. The future of CLE may be with new contrast agents to allow for molecular tagging and improved endoscopic diagnoses. The aim of this review is to describe the technology and techniques involved in CLE, and discuss the evolving applications in obtaining "virtual biopsy" throughout the GI tract.

show abstract

Diagnosing Helicobacter pylori In Vivo by Confocal Laser Endoscopy

Cited by 152 publications

References 13 publications

Confocal laser endomicroscopy for diagnosing lung cancerin vivo

Confocal laser endomicroscopy for diagnosing lung cancerin vivo

Confocal Laser Endomicroscopy: Applications in Clinical and Translational Science—A Comprehensive Review

Status of confocal laser endomicroscopy in gastrointestinal disease

Contact Info

Product

Resources

About