Diabetic hyperglycemia improves lipid profile and oxidative stress after myocardial infarction in rats (LB49)

Abstract

This study was designed to evaluate the lipid and oxidative stress profiles in diabetic rats that had undergone 15 days of myocardial infarction. Male Wistar rats were divided into control (C), diabetic (D), myocardial infarction (MI) and diabetic myocardial infarction (DMI). Left coronary artery occlusion was performed after 15 days of diabetes (STZ) and accompanied for more 15 days. The free fatty acids and triacylglycerol content and oxidative stress profile were determined by biochemical analysis in left ventricle. The free fatty acids were increased in DMI: 1.16±0.2 vs D: 0.45±0.09; MI: 0.72±0.1 and C: 0.4±0.05 mg/g of tissue, however the triacylglycerol was increased in D: 2.12±0.3 and decreased in DMI: 0.72±0.1 and MI: 1±0.2 vs C: 1.45±0.1 mg/g of tissue. The catalase activity was increased in DMI: 0.62±0.1; D: 0.56±0.09 vs C: 0.29±0.05 and MI: 0.35± 0.02 nmol/mg and glutathione peroxidase increased in DIM: 0.04±0.008 vs D: 0.02±0.003; MI: 0.02 ±0.0002 and C: 0.01±0.005 umol/min/mg. The superoxide anion and protein carbonylation were increased in MI group: 7±0.9 vs D:4±0.5; DIM:4±0.5 and C:4±0.4 O2‐mmoles/mg and MI:2.65 ± 0.36 vs D: 1.5 ± 0.24; DIM: 1.22 ± 0.2 and C: 1.45 ± 0.26 nmol/mg. These findings suggest that diabetic rats exposed to an ischemic injury showed an increase in the lipids utilization and an oxidative stress reduction, supporting previously published data of improvement of the cardiac function in these model. Grant Funding Source: Fapesp