Diabetes triggers the loss of tooth structure associated to radiographical and histological dental changes and its evolution to progressive pulp and periapical lesions in rats

Claudino, Marcela; Nunes, Isabela Sousa; Gennaro, Gabriela; Cestari, Tânia Mary; Spadella, César Tadeu; Garlet, Gustavo; Assis, Gérson Francisco de

doi:10.1016/j.archoralbio.2015.08.015

Cited by 13 publications

(24 citation statements)

References 32 publications

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“…Decreased collagen concentration has also been shown [26]. Our results are in agreement with another study performed where histological changes in pulp tissue such as reduction in collagen fibers and fibroblasts densities after 3 months of diabetes were detected in their experiments [27]. Also in agreement, a decreased concentration of collagen in dental pulp after 1 and 3 months of diabetes onset in rats was found in previous experiments [26].…”

Section: Discussionsupporting

confidence: 92%

Proliferative Capacity and Differentiation Potential of Isolated Postnatal Human Dental Pulp Stem Cells in Diabetic Patients

Shamel¹,

Ankily²,

Bakr³

2017

Stem Cells Regen Med

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Stem cells are characterized by the ability to form clones, self-regenerate and differentiate into different types of cells. Stem cells derived from human dental pulp (DPSCs) have been shown to differentiate into odontoblasts in vitro after using Bone Morphogenic Protein-2 (BMP-2). The aim of the present study was to explore the variations in the differentiation potential of the DPSC into odontoblasts in diabetic adults; it also aimed at investigating the possible influence of the origin of stem cells (coronal and radicular pulp) on their differentiation potential. Pulp tissue was extirpated from healthy individuals as well as from controlled and uncontrolled diabetic patients. Pulp tissue was divided into coronal and radicular parts and each were cultured for 30 days and then BMP-2 was added. Alizarin red staining was performed to confirm mineralization. RT-PCR was used to analyse expression of mineralization markers Dentin sialophosphoprotein (DSPP) & Enamelysin. The results of this study showed that DPSCs of both healthy and diabetic groups had stem cell properties. DPSCs of the coronal pulp have a more proliferative capacity than that of the radicular pulp. Controlled diabetes mellitus provides relatively unfavourable conditions for the DPSCs to proliferate and differentiate while uncontrolled diabetes produces more deleterious effect on the DPSCs capacity for proliferation and differentiation. Expression of DSPP and enamelysin was positive in healthy and controlled diabetics groups, while in the uncontrolled diabetic group it was negative. It is concluded that DPSCs of the coronal pulp have a more proliferative capacity than that of the radicular pulp, controlled diabetes mellitus provides relatively unfavourable conditions for the DPSCs to proliferate and differentiate and uncontrolled diabetes produces more deleterious effect on the DPSCs capacity for proliferation and differentiation.

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Section: Discussionsupporting

confidence: 92%

Proliferative Capacity and Differentiation Potential of Isolated Postnatal Human Dental Pulp Stem Cells in Diabetic Patients

Shamel¹,

Ankily²,

Bakr³

2017

Stem Cells Regen Med

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“…, Claudino et al . ), results on inflammation, dentine resorption, fibrosis, circulatory disturbances and pulp ageing remain unclear (Catanzaro et al . ).…”

Section: Introductionmentioning

confidence: 97%

“…Although there are studies on the effects of DM on pulp tissue (Leite et al 2008, Inagaki et al 2010, Claudino et al 2015, results on inflammation, dentine resorption, fibrosis, circulatory disturbances and pulp ageing remain unclear (Catanzaro et al 2006). It is known that uncontrolled diabetes results in more inflammatory reactions due to capillary leaks and cell dehydration (Bender & Bender 2003) and that diabetic pulp tissues have metabolic alterations such as altered levels of nitrite and kallikrein (Catanzaro et al 2006).…”

Section: Introductionmentioning

confidence: 99%

The effect of dental bleaching on pulpal tissue response in a diabetic animal model

et al. 2016

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“…The selected bacterial species in this study were chosen because they are commonly present in the two studied groups. DM may trigger variations in dental pulp tissue which promotes pulp necrosis [20]. …”

Section: Discussionmentioning

confidence: 99%

The Use of Different Irrigation Techniques to Decrease Bacterial Loads in Healthy and Diabetic Patients with Asymptomatic Apical Periodontitis

Ghoneim

Saber

El-Badry³

et al. 2016

Open Access Maced J Med Sci

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BACKGROUND:Diabetes mellitus is a multisystem disease which weakens the human’s immunity. Subsequently, it worsens the sequelae of apical periodontitis by raising a fierce bacterial trait due to the impaired host response.AIM:This study aimed to estimate bacterial reduction after using different irrigation techniques in systemically healthy and diabetic patients with asymptomatic apical periodontitis.MATERIAL AND METHODS:Enterococcus faecalis, Peptostreptococcus micros, and Fusobacterium necleatum bacteria were chosen, as they are the most common and prevailing strains found in periodontitis. Bacterial samples were retrieved from necrotic root canals of systemically healthy and diabetic patients, before and after endodontic cleaning and shaping by using two different irrigation techniques; the conventional one and the EndoVac system. Quantitive polymerase chain reaction (qPCR) was utilised to detect the reduction in the bacterial count.RESULTS:The EndoVac irrigation system was effective in reducing bacteria, especially Peptostreptococcus micros in the diabetic group when compared to conventional irrigation technique with a statistically significant difference.CONCLUSION:The EndoVac can be considered as a promising tool in combination with irrigant solution to defeat the bacterial colonies living in the root canal system. Additional studies ought to be done to improve the means of bacterial clearance mainly in immune-compromised individuals.

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Diabetes triggers the loss of tooth structure associated to radiographical and histological dental changes and its evolution to progressive pulp and periapical lesions in rats

Cited by 13 publications

References 32 publications

Proliferative Capacity and Differentiation Potential of Isolated Postnatal Human Dental Pulp Stem Cells in Diabetic Patients

Proliferative Capacity and Differentiation Potential of Isolated Postnatal Human Dental Pulp Stem Cells in Diabetic Patients

The effect of dental bleaching on pulpal tissue response in a diabetic animal model

The Use of Different Irrigation Techniques to Decrease Bacterial Loads in Healthy and Diabetic Patients with Asymptomatic Apical Periodontitis

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