Diabetes mellitus increased integrins gene expression in rat endometrium at the time of embryo implantation

Abstract

Background: Diabetes mellitus deeply changes the genes expression of integrin (Itg) subunits in several cells and tissues such as monocytes, arterial endothelium, kidney glomerular cells, retina. Furthermore, hyperglycemia could impress and reduce the rate of successful assisted as well as non-assisted pregnancy. Endometrium undergoes thorough changes in normal menstrual cycle and the question is: What happens in the endometrium under diabetic condition?
 Objective: The aim of the current study was to investigate the endometrial gene expression of α3, α4, αv, Itg β1 and β3 subunits in diabetic rat models at the time of embryo implantation.
 Materials and Methods: Twenty-eight rats were randomly divided into 4 groups: control group, diabetic group, pioglitazone-treated group, and metformin-treated group. Real-time PCR was performed to determine changes in the expression of Itg α3, α4, αv, β1, and β3 genes in rat’s endometrium.
 Results: The expression of all Itg subunits increased significantly in diabetic rats’ endometrium compared with control group. Treatment with pioglitazone significantly reduced the level of Itg subunits gene expression compared with diabetic rats. While metformin had a different effect on α3 and α4 and elevated these two subunits gene expression.
 Conclusion: Diabetes mellitus significantly increased the expression of studied Itg subunits, therefore untreated diabetes could be potentially assumed as one of the preliminary elements in embryo implantation failure.

Network pharmacology combined with Mendelian randomization analysis to identify the key targets of renin-angiotensin-aldosterone system inhibitors in the treatment of diabetic nephropathy

“…The impact of diabetes on the gene expression of integrin subunits is widely acknowledged, affecting various cell types and tissues including monocytes, arterial endothelial cells, glomerular cells, and the retina ( 73 ). The ITGA4 gene, which codes for α4 integrin, has been demonstrated in recent research to play a role in the PI3K-AKT signaling pathway associated with nephroprotective effects ( 74 ).…”

Protective effects of dichloroacetic acid on endometrial injury and ovarian reserve in an experimental rat model of diabetes mellitus

“…Some researchers showed that DM and related chronic hyperglycemia has unfavorable effects on endometrium and ovary, including increased risk of endometrial cancer, polycystic ovary syndrome, embryo implantation failure. [31][32][33][34] DCA administration to the diabetic rats significantly attenuates some of endometrial pathologies and endometriosis. 35,36 In this present study, we found that DCA improves the adverse effects of diabetes on the endometrium.…”

Hyaluronik Asitin Endometrium Dokusunda αVβ3 İntegrin ve Metalloproteinaz Ekspresyonuna Etkisi

“…Progesteron ve östrojenin varlığında ise bu integrinin ekspresyonun fazla olduğu saptanmıştır 18 . Ayrıca integrinlerin alt birimlerinden olan α4, αV, β1 ve β3'ün endometriumda tüm bölgelerde periyodik olarak eksprese edildiği bilinmektedir 19 . Sülz ve ark.…”