Di-n-butyl phthalate promotes lipid accumulation via the miR200c-5p-ABCA1 pathway in THP-1 macrophages

Wang, Yidi; Wen, Yuezhong; Xiao, Pingxi; Sun, Jie; Chen, Minjian; Gu, C.; Kong, Yi; Gu, Aihua; Zhang, Jingshu; Wang, Yubang

doi:10.1016/j.envpol.2020.114723

Cited by 22 publications

(14 citation statements)

References 45 publications

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“…The plasticizer dibutyl phthalate 389 and the PI3K/AKT inhibitor LY294002 421 reduced ABCA1 mRNA 389 and ABCA1 protein 389 , 421 expression and increased cellular cholesterol and lipid levels 389 in human 389 and murine 421 macrophages.…”

Section: Part I: Status Quomentioning

confidence: 96%

“… 136 Notably, radiolabeled substrates are very effective in terms of accurate tracing of protein function, as these molecules are not changed in their molecular integrity in contrast to fluorescence probes. 171 , 201 , 238 , 251 , 252 , 254 , 256 , 258 , 261 , 271 , 282 , 308 , 319 , 321 , 330 , 332 , 335 , 342 , 360 , 379 , 389 , 390 , 392 , 397 , 402 , 406 , 455 , 456 , 468 , 514 - 518 On the downside, conducting these experiments is constrained to regulatory requirements and requires extensive staff training as well as expensive safety measures and laboratory equipment.…”

Section: Part Ii: Pipeline Development To Gain Novel Diagnostics and Therapeuticsmentioning

confidence: 99%

“…Regarding fluorescent derivatives of cholesterol and phospholipids, two major types can be differentiated: (i) 7-nitro-2,1,3-benzooxadiazole (NBD) derivatives 201 , 251 , 252 , 254 , 256 , 258 , 261 , 308 , 335 , 342 , 360 , 379 , 389 , 390 , 392 , 394 , 397 , 402 , 406 , 408 , 468 and (ii) 4,4-difluoro-4-bora-3a,4a-diaza- s -indacene (BODIPY) derivatives. 271 , 282 , 319 , 321 , 330 , 332 , 455 , 456 , 515 - 517 Other fluorophore-labeled dyes have been reported, too, including the sterane analog dansylestramustine, 171 , 238 , 518 and propargyl choline, which is processed in vitro into propargylated phospholipids.…”

Section: Part Ii: Pipeline Development To Gain Novel Diagnostics and Therapeuticsmentioning

confidence: 99%

“…As a final note, it must be mentioned that other colorimetric analyses were also used to quantify the ABCA transporter-mediated function, specifically for transport of cholesterol or choline-containing lipids, using commercially available assay kits. 202 , 205 , 246 , 251 , 268 , 272 , 275 , 329 - 332 , 334 , 336 , 354 , 365 , 366 , 369 , 372 , 374 - 376 , 386 - 389 , 392 , 405 , 406 , 416 , 418 , 421 , 422 , 424 , 425 , 441 , 511 However these methodologies require time-consuming extraction processes of the lipids, and hence, are less suitable to track the function of ABCA transporters in real-time and to determine kinetic aspects of their cholesterol and lipid transport.…”

Section: Part Ii: Pipeline Development To Gain Novel Diagnostics and Therapeuticsmentioning

confidence: 99%

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Strategies to gain novel Alzheimer’s disease diagnostics and therapeutics using modulators of ABCA transporters

Pahnke

Bascuñana

Brackhan

et al. 2021

Free Neuropathology

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Adenosine-triphosphate-(ATP)-binding cassette (ABC) transport proteins are ubiquitously present membrane-bound efflux pumps that distribute endo- and xenobiotics across intra- and intercellular barriers. Discovered over 40 years ago, ABC transporters have been identified as key players in various human diseases, such as multidrug-resistant cancer and atherosclerosis, but also neurodegenerative diseases, such as Alzheimer’s disease (AD). Most prominent and well-studied are ABCB1, ABCC1, and ABCG2, not only due to their contribution to the multidrug resistance (MDR) phenotype in cancer, but also due to their contribution to AD. However, our understanding of other ABC transporters is limited, and most of the 49 human ABC transporters have been largely neglected as potential targets for novel small-molecule drugs. This is especially true for the ABCA subfamily, which contains several members known to play a role in AD initiation and progression. This review provides up-to-date information on the proposed functional background and pathological role of ABCA transporters in AD. We also provide an overview of small-molecules shown to interact with ABCA transporters as well as potential in silico , in vitro , and in vivo methodologies to gain novel templates for the development of innovative ABC transporter-targeting diagnostics and therapeutics.

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Section: Part I: Status Quomentioning

confidence: 96%

Section: Part Ii: Pipeline Development To Gain Novel Diagnostics and Therapeuticsmentioning

confidence: 99%

Section: Part Ii: Pipeline Development To Gain Novel Diagnostics and Therapeuticsmentioning

confidence: 99%

Section: Part Ii: Pipeline Development To Gain Novel Diagnostics and Therapeuticsmentioning

confidence: 99%

See 2 more Smart Citations

Strategies to gain novel Alzheimer’s disease diagnostics and therapeutics using modulators of ABCA transporters

Pahnke

Bascuñana

Brackhan

et al. 2021

Free Neuropathology

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“…They found that there was a non-linear inverted U-shaped relationship between DBP and lipid accumulation since when the THP-1 macrophages were exposed to 10 −7 mol/L of DBP, there was an increase in the lipid accumulation, by inhibiting cholesterol efflux through ABCA1 expression suppression. This may enhance the development of atherosclerosis [ 79 ]. From another perspective, the DEHP effect was analyzed on the release of tissue factor-bearing microparticles (TF-MPs).…”

Section: Human Studiesmentioning

confidence: 99%

Phthalates Implications in the Cardiovascular System

Mariana

Cairrão

2020

JCDD

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Today’s sedentary lifestyle and eating habits have been implicated as some of the causes of the increased incidence of several diseases, including cancer and cardiovascular diseases. However, environmental pollutants have also been identified as another possible cause for this increase in recent decades. The constant human exposure to plastics has been raising attention regarding human health, particularly when it comes to phthalates. These are plasticizers used in the manufacture of industrial and consumer products, such as PVC (Polyvinyl Chloride) plastics and personal care products, with endocrine-disrupting properties, as they can bind molecular targets in the body and interfere with hormonal function. Since these compounds are not covalently bound to the plastic, they are easily released into the environment during their manufacture, use, or disposal, leading to increased human exposure and enhancing health risks. In fact, some studies have related phthalate exposure with cardiovascular health, having already shown a positive association with the development of hypertension and atherosclerosis in adults and some cardiometabolic risk factors in children and adolescents. Therefore, the main purpose of this review is to present and relate the most recent studies concerning the implications of phthalates effects on the cardiovascular system.

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Dibutyl phthalate promotes angiogenesis in EA.hy926 cells through estrogen receptor-dependent activation of ERK1/2, PI3K-Akt, and NO signaling pathways

Kokai

Stanić

Tesic

et al. 2022

Chemico-Biological Interactions

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Di-n-butyl phthalate promotes lipid accumulation via the miR200c-5p-ABCA1 pathway in THP-1 macrophages

Cited by 22 publications

References 45 publications

Strategies to gain novel Alzheimer’s disease diagnostics and therapeutics using modulators of ABCA transporters

Strategies to gain novel Alzheimer’s disease diagnostics and therapeutics using modulators of ABCA transporters

Phthalates Implications in the Cardiovascular System

Dibutyl phthalate promotes angiogenesis in EA.hy926 cells through estrogen receptor-dependent activation of ERK1/2, PI3K-Akt, and NO signaling pathways

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