Di-butyl phthalate (DBP) induces defects during embryonic eye development in zebrafish

Barbagallo, Sophie; Baldauf, Cassidy; Orosco, Emily; Roy, Nicole M.

doi:10.1007/s10646-021-02468-5

Cited by 9 publications

(4 citation statements)

References 51 publications

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“…The first is linked to the absence of genes such as chokh/rx3 [52], while the second is linked to the expression of many different genes, such as sox2 [53], otx2 [54], pax6a, or pax6b [55]. Regarding chemical exposure, these two morphological aberrations have been described after treatments with a variety of chemicals, such as phenylthiourea [56], gold nanoparticles [57], di-butyl phthalate [58], and PCBs (Aroclor 1254) [59]. Retinal defects have been shown in workers exposed to solvents or heavy metal, and defects in photoreceptor cells were described in zebrafish exposed to PBDEs or PCBs [60].…”

Section: Striking Eye Malformation In Fish Treated With Any Of the Po...mentioning

confidence: 99%

A Realistic Mixture of Persistent Organic Pollutants Affects Zebrafish Development, Behavior, and Specifically Eye Formation by Inhibiting the Condensin I Complex

Guerrero-Limón

Nivelle

Bich-Ngoc

et al. 2023

Toxics

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Persistent organic pollutants (POPs) are posing major environmental and health threats due to their stability, ubiquity, and bioaccumulation. Most of the numerous studies of these compounds deal with single chemicals, although real exposures always consist of mixtures. Thus, using different tests, we screened the effects on zebrafish larvae caused by exposure to an environmentally relevant POP mixture. Our mixture consisted of 29 chemicals as found in the blood of a Scandinavian human population. Larvae exposed to this POP mix at realistic concentrations, or sub-mixtures thereof, presented growth retardation, edemas, retarded swim bladder inflation, hyperactive swimming behavior, and other striking malformations such as microphthalmia. The most deleterious compounds in the mixture belong to the per- and polyfluorinated acids class, although chlorinated and brominated compounds modulated the effects. Analyzing the changes in transcriptome caused by POP exposure, we observed an increase of insulin signaling and identified genes involved in brain and eye development, leading us to propose that the impaired function of the condensin I complex caused the observed eye defect. Our findings contribute to the understanding of POP mixtures, their consequences, and potential threats to human and animal populations, indicating that more mechanistic, monitoring, and long-term studies are imperative.

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Section: Striking Eye Malformation In Fish Treated With Any Of the Po...mentioning

confidence: 99%

A Realistic Mixture of Persistent Organic Pollutants Affects Zebrafish Development, Behavior, and Specifically Eye Formation by Inhibiting the Condensin I Complex

Guerrero-Limón

Nivelle

Bich-Ngoc

et al. 2023

Toxics

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“…Eye health: Ocular alterations induced by prenatal exposure to EDCs have been addressed in animal studies. When treated with environmentally relevant concentrations of DBP, eye development defects were reported at 96h post fertilization, such as reduced lens and retina size, poor vascularization, and loss of the optic nerve and tectum [ 133 ]. On the other hand, exposure of this phthalate to pregnant rats induced anomalies in face and eye formation, the incidence of which seemed to be higher in later generations compared to F1 [ 128 ].…”

Section: Resultsmentioning

confidence: 99%

Implications of Prenatal Exposure to Endocrine-Disrupting Chemicals in Offspring Development: A Narrative Review

Toledano,

Puche-Juarez,

Moreno-Fernandez

et al. 2024

Nutrients

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During the last decades, endocrine-disrupting chemicals (EDCs) have attracted the attention of the scientific community, as a result of a deepened understanding of their effects on human health. These compounds, which can reach populations through the food chain and a number of daily life products, are known to modify the activity of the endocrine system. Regarding vulnerable groups like pregnant mothers, the potential damage they can cause increases their importance, since it is the health of two lives that is at risk. EDCs can affect the gestation process, altering fetal development, and eventually inducing the appearance of many disorders in their childhood and/or adulthood. Because of this, several of these substances have been studied to clarify the influence of their prenatal exposure on the cognitive and psychomotor development of the newborn, together with the appearance of non-communicable diseases and other disorders. The most novel research on the subject has been gathered in this narrative review, with the aim of clarifying the current knowledge on the subject. EDCs have shown, through different studies involving both animal and human investigation, a detrimental effect on the development of children exposed to the during pregnancy, sometimes with sex-specific outcomes. However, some other studies have failed to find these associations, which highlights the need for deeper and more rigorous research, that will provide an even more solid foundation for the establishment of policies against the extended use of these chemicals.

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“…DBP can bioaccumulate in the food chain and biomagnify to high levels that threaten fish-eating wildlife and humans. It was reported that exposure to DBP had resulted in yolk sac abnormalities, skeletal defects, spinal curvatures, abnormal movement, craniofacial defects, cardiac defects, defects in eye vascularization, as well as immunotoxicity in zebrafish embryos or larvae [ 33 , 45 , 46 , 47 , 48 , 49 ].…”

Section: Introductionmentioning

confidence: 99%

Combined Toxicities of Di-Butyl Phthalate and Polyethylene Terephthalate to Zebrafish Embryos

Zhang

Zhu

2023

Toxics

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The increasing concern for the ecological risks of microplastics (MPs) as carriers of hydrophobic organic contaminants is evident. Di-butyl phthalate (DBP) is extensively utilized as an additive in plastic products, and both DBP and MPs are widespread in the environment. However, the combined toxicity of these substances remains uncertain. In this study, zebrafish embryos were employed to assess the toxic effects of polyethylene terephthalate (PET, MPs) and DBP, with a focus on the DBP toxicities influenced by PET. The embryonic chorion was partially covered by PET particles, and PET led to a delayed hatching of zebrafish embryos without inducing death or teratogenesis. On the other hand, exposure to DBP considerably inhibited the hatching of embryos, leading to severe lethal and teratogenic effects. The most common phenotypes induced by DBP exposure were delayed yolk sac absorption and pericardial edema. The mortality increased in co-treatment with 100 particles/mL PET and 2 mg/L DBP at 24 hpf and 48 hpf. The malformation phenotype, bent notochord, and delayed yolk sac absorption became more severe in 1 mg/L DBP exposition with the co-exposure of 100 particles/mL PET at 72 hpf. PET might act as a carrier that enhances the bioavailability of ambient DBP.

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Di-butyl phthalate (DBP) induces defects during embryonic eye development in zebrafish

Cited by 9 publications

References 51 publications

A Realistic Mixture of Persistent Organic Pollutants Affects Zebrafish Development, Behavior, and Specifically Eye Formation by Inhibiting the Condensin I Complex

A Realistic Mixture of Persistent Organic Pollutants Affects Zebrafish Development, Behavior, and Specifically Eye Formation by Inhibiting the Condensin I Complex

Implications of Prenatal Exposure to Endocrine-Disrupting Chemicals in Offspring Development: A Narrative Review

Combined Toxicities of Di-Butyl Phthalate and Polyethylene Terephthalate to Zebrafish Embryos

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