Di-(2-ethylhexyl) phthalate exacerbated the toxicity of polystyrene nanoplastics through histological damage and intestinal microbiota dysbiosis in freshwater Micropterus salmoides

Liao, Hongping; Liu, Shulin; Junaid, Muhammad; Gao, Dandan; Ai, Wenjie; Chen, Guanglong; Wang, Jun

doi:10.1016/j.watres.2022.118608

Cited by 32 publications

(8 citation statements)

References 62 publications

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“…As the liver is a major storage organ for glucose (as glycogen), MPs may have marked detrimental effects on systemic metabolism. Several previous studies have also reported signs of hepatic damage following MP ingestion, including cellular vacuolation, immunocyte infiltration, passive hyperemia, hydropic degeneration, dilatation and congestion of blood sinusoids, loss of parenchymal organization, focal necrosis, severe deformation of hepatocytes, and pyknotic nuclei (da Costa Araújo et al 2020 ; Espinosa et al 2019 ; Jabeen et al 2018 ; Hoseini et al 2022a , b ; Hu et al 2022 ; Karami et al 2016 ; Liao et al 2022 ; Liu et al 2022 ; Lu et al 2016 , 2022 ; Rainieri et al 2018 ; Rochman et al 2013 ; Xia et al 2020 ; Yang et al 2020 ; Yin et al 2018 ; Yu et al 2018 ). These pathological changes are also observed in response to prooxidant poisoning, and in liver diseases associated with oxidative stress (Sayed et al 2016 , 2021a , b ).…”

Section: Discussionmentioning

confidence: 95%

“…Similar to kidney and liver tissues, MP exposure also reduced tissue carbohydrate content. Previous studies have reported similar structural and pathological changes following MP exposure, including shortening, erosion, and swelling of villi; vacuolation, swelling, and blebbing of enterocytes, focal necrosis of enterocytes and mucosal epithelial cells, disruption of the epithelial boundary, complete detachment of the epithelium and lamina propria, infiltration of leukocytes, degeneration of the basement membrane, goblet cell hypertrophy, atrophy of the submucosa, pyknotic nuclei, hemorrhage, and vacuolation of mucosal cells (Ahrendt et al 2020 ; Chen et al 2022 ; Espinosa et al 2019 ; Guo et al 2022 ; Iheanacho and Odo 2020 ; Jabeen et al 2018 ; Liao et al 2022 ; Lei et al 2018 ; Lu et al 2016 ; Montero et al 2022 ; Pedà et al 2016 ; Qiao et al 2019a ; Song et al 2019 ; Xia et al 2018b ; Zhang et al 2022 ; Zhu et al 2020 ; Zuo et al 2022 ). Exposure to MPs also enhanced the number of mucosal neutrophils as revealed by PAS straining (Limonta et al 2019 ) and markedly reduced goblet cell coverage as well as gut mucus volume, indicating epithelial layer damage (Qiao et al 2019b ).…”

Section: Discussionmentioning

confidence: 99%

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Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)

Sayed

Hana

Hamed

et al. 2022

Environ Sci Pollut Res

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Microplastic particles (MPs) are a common environmental pollutant easily ingested by fish in aquaculture. The current study evaluated the protective efficacies of some antioxidant, e.g., lycopene, citric acid, and chlorella, against the toxic effects of MP ingestion by Clarias gariepinus using histopathological biomarkers. Five experimental groups were established, a control group receiving only a standard diet, a group exposed to 500 mg/kg MP concomitant with the standard diet, and three antioxidant groups exposed to MPs plus either lycopene (500 mg/kg), citric acid (30 g/kg), or chlorella (50 g/kg) in the standard diet. After 15 days, fish were sacrificed for histological and histochemical examinations. Histological analysis of the kidney for group 2 (fed 500 mg/kg MPs alone) revealed distributed tissue dissociation, regional glomerular hypertrophy or shrinkage, melanomacrophage accumulation, and expansion of Bowman’s space, while liver tissue exhibited dilation and rupture of the central vein wall, hemorrhage, cytoplasmic vacuolation, and cellular necrosis or apoptosis. Fish exposed to MPs also exhibited connective tissue fiber accumulation around renal blood vessels, renal tubules, the central hepatic vein, hepatic blood sinusoids, and serosal, muscle, and submucosal layers of the intestine. In addition, MP exposure reduced carbohydrate (mainly glycogen) contents in the brush borders and basement membranes of renal tubules, glomeruli, and intestinal tissues as well as in the cytoplasm of hepatocytes. These signs of renal, hepatic, and intestinal histopathology were fully or partially reversed by dietary lycopene, chlorella, or citric acid. Enhancing dietary antioxidants is an effective strategy for preventing MP toxicity in Clarias gariepinus in aquaculture.

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Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)

Sayed

Hana

Hamed

et al. 2022

Environ Sci Pollut Res

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“…Given their small size, NPs are easily absorbed by aquatic organisms, resulting in significant impacts on the gut microbial stability in these animals. NPs are being extensively explored as novel contaminants in aquatic animals ( Han et al, 2022 ; Liao et al, 2022 ). The current study used 16s rRNA sequencing to investigate the effects of NPs on the gut microbes of L. vannamei .…”

Section: Discussionmentioning

confidence: 99%

Effects of nanoplastics on the gut microbiota of Pacific white shrimp Litopenaeus vannamei

Zhu,

Li,

Liu

et al. 2024

PeerJ

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Nanoplastics (NPs) are an abundant, long-lasting, and widespread type of environmental pollution that is of increasing concern because of the serious threats they might pose to ecosystems and species. Identifying the ecological effects of plastic pollution requires understanding the effects of NPs on aquatic organisms. Here, we used the Pacific white shrimp (Litopenaeus vannamei) as a model species to investigate whether ingestion of polystyrene NPs affects gut microbes and leads to metabolic changes in L. vannamei. The abundance of Proteobacteria increased and that of Bacteroidota decreased after NPs treatment. Specifically, Vibrio spp., photobacterium spp., Xanthomarina spp., and Acinetobacter spp. increased in abundance, whereas Sulfitobacter spp. and Pseudoalteromonas spp. decreased. Histological observations showed that L. vannamei exposed to NP displayed a significantly lower intestinal fold height and damaged intestinal structures compared with the control group. Exposure to NPs also stimulated alkaline phosphatase, lysozyme, and acid phosphatase activity, resulting in an immune response in L. vannamei. In addition, the content of triglycerides, total cholesterol, and glucose were significantly altered after NP exposure. These results provided significant ecotoxicological data that can be used to better understand the biological fate and effects of NPs in L. vannamei.

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“…The majority of total glucose disposal happens in insulin-independent tissues, with roughly 50% occurring in the brain and 25% occurring in the splanchnic region (liver and the gastrointestinal tissues) [ 36 ]. The release of glucose from the liver closely matches glucose use, which averages around 2.0 mg/kg/min [ 37 ].…”

Section: Pathways Involved In Liver Metabolismmentioning

confidence: 99%

Nanoplastics Toxicity Specific to Liver in Inducing Metabolic Dysfunction—A Comprehensive Review

Haldar

Yhome

Muralidaran

et al. 2023

Genes

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Plastic pollution in the world is widespread and growing. The environment is swamped with nanoplastics (<100 nm), and the health consequences of these less visible pollutants are unknown. Furthermore, there is evidence that microplastics can release nanoplastics by digestive disintegration, implying that macroplastic exposure can cause direct and indirect disease via nanoplastics. The existence and impact of nanoplastics in numerous tissues from invertebrates to larger vertebrates that consume significant amounts of plastics were investigated, and histopathological techniques were utilized to determine physiological reactions and inflammation from the plastics. Nanoplastics enters an organism through the respiratory and gastro-intestinal tract where they accumulate into the liver through blood circulation via absorption, or epidermal infiltration. It is stated that macroplastics can cause damage directly at the site of exposure, whereas nanoplastics can influence the liver, causing subsequent damage to other organs. Multi-organ dysfunction is brought on by liver changes, and nanoplastics can readily enter the gut-liver axis and disturb the gut microflora. By exploring the literature and summarizing the research that has been published to date, this review article reveals the deleterious effect and mechanisms of nanoplastics on the pathophysiological functions of the hepatic system.

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Di-(2-ethylhexyl) phthalate exacerbated the toxicity of polystyrene nanoplastics through histological damage and intestinal microbiota dysbiosis in freshwater Micropterus salmoides

Cited by 32 publications

References 62 publications

Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)

Protective efficacy of dietary natural antioxidants on microplastic particles-induced histopathological lesions in African catfish (Clarias gariepinus)

Effects of nanoplastics on the gut microbiota of Pacific white shrimp Litopenaeus vannamei

Nanoplastics Toxicity Specific to Liver in Inducing Metabolic Dysfunction—A Comprehensive Review

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