DHEA prevents bone loss by suppressing the expansion of CD4&lt;sup&gt;+&lt;/sup&gt; T cells and TNFa production in the OVX-mouse model for postmenopausal osteoporosis

Zhang, Na; Gui, Yuyan; Tang, Wei; Li, Lisha; Gober, Hans-Jürgen; Li, Da‐Jin; Wang, Ling

doi:10.5582/bst.2016.01081

Cited by 13 publications

(7 citation statements)

References 45 publications

(51 reference statements)

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“…Additionally, we did not find a statistically significant relationship between the use of ART and LBMD or between the duration of ART and LBMD, although both relationships have been already reported 18 , 19 . As mentioned before, osteoporosis was associated with HIV infection duration, low current or low nadir CD4 cell count or high viral load, for example 20 . Cazanave et al .…”

Section: Discussionmentioning

confidence: 70%

Low bone mineral density among HIV-infected patients in Brazil

Chaba

Soares

Pereira

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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Decrease in bone mineral density (BMD) has been a complication among people living with HIV/AIDS. To investigate the prevalence of osteopenia/osteoporosis among HIV-infected people living in São Paulo city, we studied 108 HIV-infected patients (79 men and 29 women). We extracted data from patients’ medical records and BMD was measured by dual-energy X-ray absorptiometry (DXA). Median age of participants was 42 years (interquartile range [IQR] 36-48 years), and the median time since HIV diagnosis was 4.01 years (IQR 2-11 years). Patients had acquired HIV primarily by the sexual route (men who have sex with men 44%, heterosexual 49%). Median age, duration of HIV infection, duration of ART and CD4 nadir were similar for men and women. Plasma viral load was undetectable for 53 patients (49%). Median CD4 T cell count was 399 cells/µL (IQR 247 - 568). Twenty five patients (23%) had LBMD, and there was no statistically significant difference between men and women (<-1). The associated risk factors for LBMD were older age (≥ 50 years old) and smoking with a RR of 3.87 and 2.80, respectively. Thus, despite the lack of statistically significant relationship between the use of ART and LBMD or between duration of ART and LBMD, these factors should be addressed in larger studies.

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Section: Discussionmentioning

confidence: 70%

Low bone mineral density among HIV-infected patients in Brazil

Chaba

Soares

Pereira

et al. 2017

Rev. Inst. Med. trop. S. Paulo

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“…Three‐month‐old female mice in SPF grade were divided into 6 groups: blank group, Sham group, OVX group, OVX + IL‐7 group (caudally intravenous injection of IL‐7 recombinant protein (10 mg/kg) after OVX), OVX + IL‐7R siRNA group (caudally intravenous injection of 3.75 mg/kg IL‐7R siRNA delivered via (DSS) 6‐liposome after OVX) and OVX + c‐Fos siRNA group (caudally intravenous injection of 3.75 mg/kg c‐Fos siRNA delivered via (DSS) 6‐liposome after OVX). The OVX osteoporosis rat model was constructed based on the method of Zhang et al (). The Sham group only had a ligation of the fallopian tube without an ovariectomy.…”

Section: Methodsmentioning

confidence: 99%

Effects of interleukin‐7/interleukin‐7 receptor on RANKL‐mediated osteoclast differentiation and ovariectomy‐induced bone loss by regulating c‐Fos/c‐Jun pathway

Zhao

Zhaofeng

et al. 2018

Journal Cellular Physiology

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To explore the effects of IL-7/IL-7R on the RANKL-mediated osteoclast differentiation in vitro and OVX-induced bone loss in vivo. BMMs and RAW264.7 were transfected with IL-7, IL-7R siRNA, c-Fos siRNA, and c-jun siRNA and later stimulated by RANKL. TRAP and toluidine blue staining were used to observe osteoclast formation and bone resorption, respectively. HE and TRAP staining were used to detect trabecular bone microstructure and osteoclasts of mice, respectively. qRT-PCR and Western blot analysis were used to examine expression. IL-7 unregulated the expression of CTSK, NFATc1, MMP9, and the phosphorylation of p38 and Akt by activating the c-Fos/c-Jun pathway, which increased osteoclast numbers and bone resorption in RANKL-stimulated macrophages. While IL-7R siRNA and c-Fos siRNA decreased the expression, as well as and the phosphorylation of p38 and Akt.IL-7 decreased the BMD and OPG expression in OVX-induced mice and increased the TRAP positive cells, the mRNA expression of c-fos, c-jun, and RANKL, which was contradictory to IL-7R siRNA, and c-Fos siRNA. Furthermore, IL-7R siRNA and c-Fos siRNA caused thicker trabeculae, increased trabecular number, and decreased osteolysis in OVX mice. IL-7/IL-7R can promote RANKL-mediated osteoclast formation and bone resorption by activating the c-Fos/c-Jun pathway, as well as inducing bone loss in OVX mice.

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“…28 Moreover, both DHEA and BSNXD were thought to have an immuno-regulatory role because DHEA decreased tumor necrosis factor (TNF)-α production and CD4 þ cells growth rate and BSNXD suppressed the increase of CD4 þ , CD8 þ cells while it increased Tregs in OVX mice. 27,33 Tregs, from peripheral blood mononuclear cells, suppressed osteoclast differentiation and bone resorption through the secretion of interleukin (IL)-10 and transforming growth factor (TGF)-β1 in human bone marrow cells. 34 This further explanation added weight to the cross-talk between the immune system and bone metabolism.…”

Section: Chinese Herbal Formulas For Postmenopausal Osteoporosis 77mentioning

confidence: 99%

Chinese herbal formulas for postmenopausal osteoporosis: A review of preclinical evidence on animal studies and molecular mechanism

Lin

Tian

et al. 2018

Tradit. Med. Mod. Med.

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Whether Chinese herbal formulas are effective in treatment of postmenopausal osteoporosis remains unclear. The aim of this study is to explore the experimental evidence of both in vitro and in vivo preclinical studies using Chinese herbal formulas in postmenopausal osteoporosis. Searches were applied to various databases with relevant keywords. Original in vivo and in vitro studies using Chinese herbal formulas to treat postmenopausal osteoporosis, and with full text available, were included. Er-Xian Decoction, Bu-Shen-Ning-Xin Decoction, Qing E Formula, Liuwei Dihuang Wan, and Xian-Ling-Gu-Bao Decoction, the most commonly studied formulas, were selected from the pool of Chinese medicine. The preclinical data indicated the potential use of Chinese herbal formulas in postmenopausal osteoporosis. The underlying mechanisms included bone morphogenetic protein (BMP), Wnt/[Formula: see text]-catenin, extracellular-signal-regulated kinase/c-Jun [Formula: see text] terminal kinase/mitogen-activated protein kinase (ERK/JNK/MAPK), estrogen receptor (ER), and osteoprotegerin/receptor activator of nuclear factor [Formula: see text]B ligand (OPG/RANKL) signaling pathways. This study demonstrated the anti-osteoporotic effect of Chinese herbal formulas targeting different pathways in bone metabolism. Further study with adequate sample size and follow-up time, appropriate controls, and optimal blinding is required.

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DHEA prevents bone loss by suppressing the expansion of CD4<sup>+</sup> T cells and TNFa production in the OVX-mouse model for postmenopausal osteoporosis

Cited by 13 publications

References 45 publications

Low bone mineral density among HIV-infected patients in Brazil

Low bone mineral density among HIV-infected patients in Brazil

Effects of interleukin‐7/interleukin‐7 receptor on RANKL‐mediated osteoclast differentiation and ovariectomy‐induced bone loss by regulating c‐Fos/c‐Jun pathway

Chinese herbal formulas for postmenopausal osteoporosis: A review of preclinical evidence on animal studies and molecular mechanism

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