DFD-01 Reduces Transepidermal Water Loss and Improves Skin Hydration and Flexibility

Abstract

IntroductionIn plaque psoriasis, the benefit of topical steroids is well established. The vehicle formulation of topical steroids may also provide benefit in addition to the effects of the steroid itself. DFD-01 (betamethasone dipropionate spray, 0.05%) is a formulation composed of a topical steroid in an emollient-like vehicle that enhances penetration to the target site of inflammation in the skin. The aim of this study was to assess the effect of DFD-01 and its vehicle on skin hydration and barrier function in compromised skin and to evaluate its effect on flexibility in healthy skin.MethodsEighteen healthy white volunteers were enrolled in each of two studies. In Study 1, dry shaving of volar forearms created a compromised skin barrier, through which transepidermal water loss (TEWL) was measured using an evaporimeter. Capacitance, a measure of epidermal hydration, was also measured at baseline and at 1, 2 and 4 h after application of DFD-01 or its vehicle formulation. In Study 2, intact skin flexibility was tested with a cutometer before and at 1, 2 and 4 h after application of DFD-01 or vehicle.ResultsIn Study 1, both DFD-01 and its vehicle were effective at reducing TEWL through the compromised stratum corneum. Capacitance measurements confirmed this finding; razor-chafed skin treated with either DFD-01 or vehicle exhibited levels of skin hydration similar to unshaved control skin. Study 2 found softening and greater flexibility of normal skin treated with either DFD-01 or vehicle compared with nontreated control skin samples.ConclusionsThese tests suggest that the DFD-01 formulation and its vehicle are each effective at retaining moisture within a damaged skin barrier and for softening and increasing the flexibility of intact skin.FundingDr. Reddy’s Laboratories.

Unsaturated fatty acid‐enriched extract from Hippophae rhamnoides seed reduces skin dryness through up‐regulating aquaporins 3 and hyaluronan synthetases 2 expressions

“…It will further cause chronic itch and even affect quality of life. Dryness was obvious associated with eczematous or psoriatic skin, 26 transported by AQP3 also has been proved that induced different early differentiating markers expressions. 32 Nevertheless, the role of AQP3 in cell proliferation and differentiation was associated with various skin diseases.…”

“…It will further cause chronic itch and even affect quality of life. Dryness was obvious associated with eczematous or psoriatic skin, 26 and maintaining adequate skin hydration in the skin requires effort from two aspects: (a) skin barrier function improvement, such as reinforcing cornified envelope and order intercellular lamellar lipids to reduce TWEL, or increasing the content of natural moisturizing factors (NMF) in corneocytes; (b) intrinsic hydration capacity enhancement, such as increasing content of endogenous glycerol, which served as a natural moisturizer, and HA in the skin 10,27 . AQP3 is abundantly expressed in epidermis, served as a channel to transport glycerol and water 6 ; HAS2 is also expressed by keratinocyte and located in epidermis, which is responsible for the synthesis of large molecules of HA 16 .…”