Dextran sulfate-Mg2+ precipitation procedure for quantitation of high-density-lipoprotein cholesterol.

Glycaemic control and familial factors determine hyperlipidaemia in early childhood diabetes

“…Between batch precision (% coef®cient of variation (CV)) for T-chol was 2.6% at 2.82 mmol/l and 2.3% at 5.66 mmol/l and for Tag 3.2% at 1.01 mmol/l and 2.1% at 3.15 mmol/l. The precipitation procedure used for high density lipoprotein (HDL)-cholesterol was the dextran sulphate±manganese chloride method of Warnick et al [10] and very low density lipoprotein (VLDL)cholesterol used sodium dodecyl sulphate (SDS). The between batch CV for HDL-cholesterol was 6.1% at 0.95 mmol/l and 4.1% at 2.04 mmol/l.…”

Elevated leptin concentrations in growth hormone‐deficient hypopituitary adults

“…Triglyceride and cholesterol levels were determined enzymatically using a Centrifichem centrifugal analyser (Baker, Windsor, UK). High density lipoprotein (HDL) was analysed following dextran sulphate extraction (Warnick et al, 1982). Low density lipoprotein (LDL) cholesterol was calculated by Friedwald formula.…”

GH levels and insulin sensitivity are differently associated with biomarkers of cardiovascular disease in active acromegaly

“…HDL was isolated in the supernatant obtained following precipitation of apo B-containing lipoproteins with 40 g/l of phosphotungstic acid in the presence of magnesium ions. 11 Within-run and between-day precisions (CV) were 3·2% and 3·8%, respectively. Apo A-I and apo B were evaluated by immunoturbidimetry (Roche Diagnostics) in a Hitachi 917 autoanalyzer.…”