Dextran stabilized fullerene soot induced toxicity on alveolar epithelial cells (A549 cells)

Athira, S.S.; Biby, E.T.; Mohanan, P.V.

doi:10.1016/j.envres.2020.109716

Cited by 9 publications

(4 citation statements)

References 43 publications

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“…Dilute DCFH-DA 1:1000 with PBS buffer to make the final concentration 10 uM. DCFH-DA completely immersed the cotton leaves and reacted at 28 °C for 20 min [ 70 ]. Before observation, the leaves were washed with double distilled water at least three times to remove the reagents on the surface and then observed the staining signal with a laser confocal microscope [ 71 ].…”

Section: Methodsmentioning

confidence: 99%

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Zhang

Wang

Yang

et al. 2022

IJMS

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Verticillium wilt (VW), a fungal disease caused by Verticillium dahliae, currently devastates cotton fiber yield and quality seriously, yet few resistance germplasm resources have been discovered in Gossypium hirsutum. The cotton variety Nongda601 with suitable VW resistance and high yield was developed in our lab, which supplied elite resources for discovering resistant genes. Early nodulin-like protein (ENODL) is mainly related to nodule formation, and its role in regulating defense response has been seldom studied. Here, 41 conserved ENODLs in G. hirsutum were identified and characterized, which could divide into four subgroups. We found that GhENODL6 was upregulated under V. dahliae stress and hormonal signal and displayed higher transcript levels in resistant cottons than the susceptible. The GhENODL6 was proved to positively regulate VW resistance via overexpression and gene silencing experiments. Overexpression of GhENODL6 significantly enhanced the expressions of salicylic acid (SA) hormone-related transcription factors and pathogenicity-related (PR) protein genes, as well as hydrogen peroxide (H2O2) and SA contents, resulting in improved VW resistance in transgenic Arabidopsis. Correspondingly, in the GhENODL6 silenced cotton, the expression levels of both phenylalanine ammonia lyase (PAL) and 4-coumarate-CoA ligase (4CL) genes significantly decreased, leading to the reduced SA content mediating by the phenylalanine ammonia lyase pathway. Taken together, GhENODL6 played a crucial role in VW resistance by inducing SA signaling pathway and regulating the production of reactive oxygen species (ROS). These findings broaden our understanding of the biological roles of GhENODL and the molecular mechanisms underlying cotton disease resistance.

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Section: Methodsmentioning

confidence: 99%

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Zhang

Wang

Yang

et al. 2022

IJMS

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“…For this reason, the nanofilm at a concentration of 100 mg/L was used in further research. It seems that the structure of fullerenes determines their toxicity, and carbon soot C 60 / 70 with a predominance of C 70 , even at low concentrations, induces the necrosis of mouse fibroblast cells L929, rat glioma C6, human glioma U251 [26] and human lung cancer A549 [27]. Cell death after exposure to high doses of C 60 fullerene may be associated with ROS-dependent cell membrane damage, as previously described for melanoma cells B16 [28].…”

Section: Discussionmentioning

confidence: 62%

Influence of C60 Nanofilm on the Expression of Selected Markers of Mesenchymal–Epithelial Transition in Hepatocellular Carcinoma

Sosnowska,

Kutwin,

Zawadzka

et al. 2023

Cancers

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The epithelial–mesenchymal transition (EMT) is a process in which epithelial cells acquire the ability to actively migrate via a change to the mesenchymal phenotype. This mechanism occurs in an environment rich in cytokines and reactive oxygen species but poor in nutrients. The aim of this study was to demonstrate that the use of a fullerene C60 nanofilm can inhibit liver cancer cell invasion by restoring their non-aggressive, epithelial phenotype. We employed epithelial and mesenchymal HepG2 and SNU-449 liver cancer cells and non-cancerous mesenchymal HFF2 cells in this work. We used enzyme-linked immunosorbent assays (ELISAs) to determine the content of glutathione and transforming growth factor (TGF) in cells. We measured the total antioxidant capacity with a commercially available kit. We assessed cell invasion based on changes in morphology, the scratch test and the Boyden chamber invasion. In addition, we measured the effect of C60 nanofilm on restoring the epithelial phenotype at the protein level with protein membranes, Western blotting and mass spectrometry. C60 nanofilm downregulated TGF and increased glutathione expression in SNU-449 cells. When grown on C60 nanofilm, invasive cells showed enhanced intercellular connectivity; reduced three-dimensional invasion; and reduced the expression of key invasion markers, namely MMP-1, MMP-9, TIMP-1, TIMP-2 and TIMP-4. Mass spectrometry showed that among the 96 altered proteins in HepG2 cells grown on C60 nanofilm, 41 proteins are involved in EMT and EMT-modulating processes such as autophagy, inflammation and oxidative stress. The C60 nanofilm inhibited autophagy, showed antioxidant and anti-inflammatory properties, increased glucose transport and regulated the β-catenin/keratin/Smad4/snail+slug and MMP signalling pathways. In conclusion, the C60 nanofilm induces a hybrid mesenchymal–epithelial phenotype and could be used in the prevention of postoperative recurrences.

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“…Reports are available on the context that this fullerene soot fine powder is capable of imposing scrotal cancer in chimney sweepers [10,11]. Toxic consequences of fullerene soot nanoparticles were also evident in lung epithelial cells [12]. Direct applications of fullerenes and their derivatives have found promising applicability in various medicinal as well as industrial fields.…”

Section: Properties and Applicationsmentioning

confidence: 99%

Glial Cell Colony Injured on Exposure to Fullerene Soot Nanoparticles

2020

Lett Appl NanoBioSci

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An expansion in the field of nanotechnology and their applications in medicine and pharmaceuticals have revolutionized the 20th century. Principally it deals with particles exhibiting a size below 100nm at least in a single dimension. Various fields that gain application potentials of nanotechnology include health and medicine, electronics, energy and environment, space research, etc. Advancements in experimental techniques have to lead to the development of multi functionalized nanoparticles like fullerenes, which is one of the allotropic forms of carbon. Fullerene was initially fabricated by laser ablation of graphite in an inert atmosphere. The crude early form consists of a mixture of interlocked carbon cages in its impure version, referred to as fullerene soot. Nature gets exposed to fullerene soot from a cluster of sources, including vehicle, industrial, and other household exhausts. An increasing level of exposure requires toxicity evaluation of such materials in biological systems. Present study addresses in vitro toxicity studies of fullerene soot nanoparticles in C6 glial cells. The study particularly analyzes cell viability assays, morphological evaluations, organelle functionality analysis, live-dead assays, and nuclear integrity measurement. The study confirms the dose-dependent toxicity of the nanomaterials, which further recommends proper precautions to be taken during exposure occasions.

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Dextran stabilized fullerene soot induced toxicity on alveolar epithelial cells (A549 cells)

Cited by 9 publications

References 43 publications

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

GhENODL6 Isoforms from the Phytocyanin Gene Family Regulated Verticillium Wilt Resistance in Cotton

Influence of C60 Nanofilm on the Expression of Selected Markers of Mesenchymal–Epithelial Transition in Hepatocellular Carcinoma

Glial Cell Colony Injured on Exposure to Fullerene Soot Nanoparticles

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