Dextran hydrolysis by a Fusobacterium strain isolated from human dental plaque

Costa, Telma; Bier, L.C.; Гайда, Ф.А.

doi:10.1016/0003-9969(74)90200-3

Cited by 20 publications

(13 citation statements)

References 9 publications

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“…in particular, has been linked to gingivitis, a general-ized inflammation of the gingival tissues (25,34,39), Pertinent to the possible contribution of F nucleatum to the development of gingivitis and some forms of periodontal disease this baeterium produces various products, such as significant amounts of butyric acid (26), hydrogen sulfide, dimethyl disulfide, dimethyl trisulfide and methyl mercaptan (19, unpublished observations), that have the potential to cause eellular damage. In addition, it has been reported that some strains produce collagenase (26) and dextranase (6),…”

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confidence: 99%

Evidence for the existence of two classes of corncob (coaggregation) receptor in Fusobacterium nucleatum

Kaufman

DiRienzo

1988

Oral Microbiology and Immunology

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Oral isolates of Fusobacterium nucleatum have the ability to form distinct coaggregation units, termed corncobs, when mixed with Streptococcus sanguis. Saturation binding kinetics determined for 12 strains of F. nucleatum revealed that these strains could be divided into two groups. One group, represented by F. nucleatum ATCC 10953 reproducibly bound twice as many streptococci as the other group, exemplified by F. nucleatum FDC 364. Other Fusobacterium species tested, including F. mortiferum, Fusobacterium periodonticum, Fusobacterium simiae and Fusobacterium necrophporum, failed to form corncobs with S. sanguis. In inhibition experiments lipoteichoic acid (LTA)‐enriched cell extracts from S. sanguis blocked corncob formation between this bacterium and strain FDC 364 with material containing as little as 50 ng phosphate exhibiting greater than 50% inhibition. Alternatively, 40 times as much material failed to inhibit corncob formation between S. sanguis and F. nucleatum ATCC 10953 or Bacterionema matruchotti. Deacylation of the LTA destroyed its ability to inhibit the fusobacterial coaggregation but produced an active inhibitor in the case of B. matruchotii corncobs. Concerning the fusobacterial coaggregation partner, membrane fractions obtained from strain ATCC 10953 blocked corncob formation while the inhibitory activity of strain FDC 364 was confined to the soluble protein fraction. Corncob‐deficient mutants of F. nucleatum FDC 364 were produced by treating the cells with acridine orange. However, this agent had no effect on the corncob‐forming ability of strain ATCC 10953. The results of these experiments provide convincing evidence that at least 2 independent strain‐specific receptors on F. nucleatum are involved in corncob formation between this bacterium and S. sanguis.

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confidence: 99%

Evidence for the existence of two classes of corncob (coaggregation) receptor in Fusobacterium nucleatum

Kaufman

DiRienzo

1988

Oral Microbiology and Immunology

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“…A gram negative filamentous rod, possibly a Fusobacterium spp. [Costa et al, 1974], and an unidentified gram-positive pleomorphic rod were the only other DP bacteria detected in the clinical samples.…”

Section: Resultsmentioning

confidence: 99%

In vivo Relationships of the Dextran-Degrading Oral Microbiota to Streptococcus mutans and Caries Experience

1982

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Several dextranase-producing (DP) microorganisms indigenous to ths oral cavity reduce in vitro adherence of Streptococcus mutans to smooth surfaces. In this report, the DP microorganisms were studied to determine if they had an influence on the in vivo S. mutans population and the development of carious lesions in children. The epidemiological investigation showed that the oral cavity of all children sampled harbored dextranolytic microbes and that the highest concentrations were recovered from saliva. Estimates of correlation calculated from the data produced no significant antagonistic relationships between the DP bacteria found in saliva, smooth surface plaque and interproximal plaque and either the DMFS index or the S. mutans population. It was concluded that dextranase-producing microorganisms did not detectably influence the in vivo ecology of S. mutans, possibly because recent evidence indicates a nonglucan molecule may be responsible for initial attachment of S. mutans to smooth surfaces.

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“…A wide range of bacterial species, such as Bacteroides spp., Bifidobacterium spp., and Fusobacterium spp. associated with dental plaque, produce inducible dextran-hydrolyzing enzymes (26,76,86,196,205).…”

Section: Role Of Dextranases In Non-dextran-producing Microorganismsmentioning

confidence: 99%

Microbial Dextran-Hydrolyzing Enzymes: Fundamentals and Applications

Khalikova

Susi

Korpela

2005

Microbiol Mol Biol Rev

226

147

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Dextran is a chemically and physically complex polymer, breakdown of which is carried out by a variety of endo- and exodextranases. Enzymes in many groups can be classified as dextranases according to function: such enzymes include dextranhydrolases, glucodextranases, exoisomaltohydrolases, exoisomaltotriohydrases, and branched-dextran exo-1,2-α-glucosidases. Cycloisomalto-oligosaccharide glucanotransferase does not formally belong to the dextranases even though its side reaction produces hydrolyzed dextrans. A new classification system for glycosylhydrolases and glycosyltransferases, which is based on amino acid sequence similarities, divides the dextranases into five families. However, this classification is still incomplete since sequence information is missing for many of the enzymes that have been biochemically characterized as dextranases. Dextran-degrading enzymes have been isolated from a wide range of microorganisms. The major characteristics of these enzymes, the methods for analyzing their activities and biological roles, analysis of primary sequence data, and three-dimensional structures of dextranases have been dealt with in this review. Dextranases are promising for future use in various scientific and biotechnological applications

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Dextran hydrolysis by a Fusobacterium strain isolated from human dental plaque

Cited by 20 publications

References 9 publications

Evidence for the existence of two classes of corncob (coaggregation) receptor in Fusobacterium nucleatum

Evidence for the existence of two classes of corncob (coaggregation) receptor in Fusobacterium nucleatum

In vivo Relationships of the Dextran-Degrading Oral Microbiota to Streptococcus mutans and Caries Experience

Microbial Dextran-Hydrolyzing Enzymes: Fundamentals and Applications

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