Dextran 40 reduces<i>in vitro</i>platelet aggregation in peripheral arterial disease

Robless, P; Okonko, Darlington O.; Mikhailidis, DP; Stansby, Gerard

doi:10.1080/09537100410001682814

Cited by 34 publications

(20 citation statements)

References 28 publications

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“…Dextrans also have a platelet inhibiting effect, which can be used in peripheral arterial surgery to counteract thrombosis [31]. In a recent review by Hartog et al [11], 17 in vitro studies clearly indicate that HES results in a weaker and smaller clot measured with thrombelastography.…”

Section: Discussionmentioning

confidence: 98%

Albumin-induced coagulopathy is less severe and more effectively reversed with fibrinogen concentrate than is synthetic colloid-induced coagulopathy

Winstedt

Hanna

Schött

2013

Scandinavian Journal of Clinical and Laboratory Investigation

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Introduction Autophagy is well known as one of the biogenic responses against various stresses, which possesses the benefi cial roles for survival, but little is known about the dynamics and its signifi cance during the septic condition. We hypothesized that autophagy is induced during the septic condition, and contributes to protect from tissue damage which subsequently leads to organ dysfunction. We confi rm whether the autophagic process is accelerated or sustained in an acute phase of sepsis and we also determine its physiological role. Methods Sepsis was induced by cecal ligation and puncture (CLP) in mice. We examined the kinetics of autophagosome and auto lysosome formation which may explain the status of autophagy by western blotting, immunohistochemistry, and electron microscopy. To investigate a precise role of autophagy in CLP-induced sepsis, chloroquine, an autophagy inhibitor, was administered to the CLP-operated mice, and blood chemistry, pathology of the liver and survival were evaluated. Results Autophagy demonstrated by the ratio of LC3-II/LC3-I was induced over the time course up to 24 hours after CLP. The ratio was particularly increased in the liver, heart and spleen. Autophagosome formation became maximal at 6 hours and declined by 24 hours after CLP. Autolysosome formation as evaluated by both fusion of GFP-LC3 dots with LAMP1 immunohistochemistry and electron microscopy was also increased after the procedure. Furthermore, inhibition of autophagy by chloroquine during the CLP procedure resulted in elevation of serum AST levels, and signifi cantly increased mortality in mice. Conclusion Autophagy was induced in several organs over the time course of the CLP sepsis model and then the process was gradually completed to degradation of the components. Our data suggest autophagy plays a protective role in organ dysfunction in sepsis. P2Reversible depressive eff ect of TNFα on a model of isolated perfused rat heart BV Nguyen Introduction Acute myocardial depression in septic shock is common [1]. Myocardial depression is mediated by circulating depressant substances, which until now have been incompletely characterized [2].The aim of our study was to observe the eff ects of TNFα on the model of perfused rat heart. Methods After profound anesthesia with pentothal, the Wistar rats were killed by exsanguination. After sternotomy, the heart was taken and connected to the Langendorf column. The apex of the heart was hooked to a strength sensor. Biopac student laboratory software was used to record and analyse heart contractions. Contractions were recorded every 5 minutes during periods of 20 minutes. Control measurements were fi rst recorded. We measured four parameters: heart rate, contraction force, speeds of contraction and relaxation for control, during TNFα (20 ng/ml) exposure and after removal of TNFα. We express the variations of parameters as percentage of the control ± SEM. A paired t test was used to compare heart rate, contraction amplitude, speeds of contraction and relaxation with TNFα and ...

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Section: Discussionmentioning

confidence: 98%

Albumin-induced coagulopathy is less severe and more effectively reversed with fibrinogen concentrate than is synthetic colloid-induced coagulopathy

Winstedt

Hanna

Schött

2013

Scandinavian Journal of Clinical and Laboratory Investigation

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“…The polymer dextran has been shown to possess anti-thrombotic and anti-platelet function [24]. A few studies have previously reported on the ability of dextran to reduce agonist induced platelet aggregation [25,26]. Platelets absorb dextran, with resultant changes in electrophoretic mobility; suggesting that the inhibitory effect of dextran on platelet function might be due to some alteration of platelet membrane function [27].…”

Section: Discussionmentioning

confidence: 99%

In vitro hematological and in vivo immunotoxicity assessment of dextran stabilized iron oxide nanoparticles

Easo

Mohanan

2015

Colloids and Surfaces B: Biointerfaces

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“…Plant tissues and cell cultures [Su, 1995] have been exploited for the production of useful products derived from cell walls, such as wood, biomaterials, and biomedical materials [Murdoch et al, 2004;Robless et al, 2004]. These products and their cells have unique properties, including high elasticity with low density, low thermal conductivity, and high biomedical adaptability.…”

Section: Introductionmentioning

confidence: 99%

Magnetic field exposure stiffens regenerating plant protoplast cell walls

Haneda

Fujimura

Iino

2006

Bioelectromagnetics

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Single suspension-cultured plant cells (Catharanthus roseus) and their protoplasts were anchored to a glass plate and exposed to a magnetic field of 302 +/- 8 mT for several hours. Compression forces required to produce constant cell deformation were measured parallel to the magnetic field by means of a cantilever-type force sensor. Exposure of intact cells to the magnetic field did not result in any changes within experimental error, while exposure of regenerating protoplasts significantly increased the measured forces and stiffened regenerating protoplasts. The diameters of intact cells or regenerating protoplasts were not changed after exposure to the magnetic field. Measured forces for regenerating protoplasts with and without exposure to the magnetic field increased linearly with incubation time, with these forces being divided into components based on the elasticity of synthesized cell walls and cytoplasm. Cell wall synthesis was also measured using a cell wall-specific fluorescent dye, and no changes were noted after exposure to the magnetic field. Analysis suggested that exposure to the magnetic field roughly tripled the Young's modulus of the newly synthesized cell wall without any lag.

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Dextran 40 reducesin vitroplatelet aggregation in peripheral arterial disease

Cited by 34 publications

References 28 publications

Albumin-induced coagulopathy is less severe and more effectively reversed with fibrinogen concentrate than is synthetic colloid-induced coagulopathy

Albumin-induced coagulopathy is less severe and more effectively reversed with fibrinogen concentrate than is synthetic colloid-induced coagulopathy

In vitro hematological and in vivo immunotoxicity assessment of dextran stabilized iron oxide nanoparticles

Magnetic field exposure stiffens regenerating plant protoplast cell walls

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