Dexamethasone Treatment Increases the Intracellular Calcium Level Through TRPV6 in A549 Cells

Abstract

This study investigated the effect of dexamethasone (DEX) on intracellular calcium levels and the expressions of transient receptor potential cation channel subcomponent V member 6 (TRPV6), sodium-calcium exchanger 1 (NCX1), and plasma membrane calcium ATPase 1 (PMCA1) in A549 cells. The intracellular calcium level, by using the calcium indicator pGP-CMV-GCaMP6f, increased following DEX treatment for 6, 12, and 24 h in A549 cells. In addition, Rhod-4 assay after DEX treatment for 24 h showed that DEX increased the level of intracellular calcium. The expression of the calcium influx TRPV6 gene significantly increased, whereas the expressions of the calcium outflow NCX1 and PMCA1 genes significantly decreased with DEX treatment. The mRNA levels of surfactant protein genes SFTPA1, SFTPB, SFTPC, and SFTPD and the secreted airway mucin genes MUC1 and MUC5AC were investigated by treating cells with DEX. The DEX treatment decreased the mRNA levels of SFTPA1 and SFTPB but increased the mRNA levels of SFTPC and SFTPD. The MUC1 mRNA level was increased by DEX treatment, whereas MUC5AC mRNA was significantly decreased. These results indicate that DEX influences the intracellular calcium level through TRPV6, and affects pulmonary surfactant genes and secreted airway mucin genes in A549 cells.

TREVIXMBD: A Transcriptional Regulation-driven Variational Inference Model to Speculate Gene Expression Mechanism with Integration of Single-cell Multi-omics

“…D. The boxplot of the Silhouette coefficient.Next, we investigated the capacity of TREVI XMBD to capture subtle temporal dynamics in perturbed cells of the same cell type, aiming to discern nuanced changes. For this purpose, we utilized the A549 dataset, comprising cells derived from the human non-small cell lung cancer (NSCLC) cell line, treated with Dexamethasone (DEX), and sampled at three distinct time points (0, 1, and 3 hour)[18]. Employing multi-omics sequencing, the dataset probed the impact of DEX on the evolution and reprogramming of human NSCLC cells, a process characterized by gradual, subtle alternations in cellular genetic elements.…”