Dexamethasone Suppresses Histamine Synthesis by Repressing both Transcription and Activity of HDC in Allergic Rats

Kitamura, Yoshiaki; Das, Asish Kumar; Murata, Yuki; Maeyama, Kazutaka; Dev, Shrabanti; Wakayama, Yousuke; Kalubi, Bukasa; Takeda, Noriaki; Fukui, Hirokazu

doi:10.2332/allergolint.55.279

Cited by 27 publications

(34 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As histidine decarboxylase (HDC) is the only enzyme that synthesizes histamine from histidine in mammals, control of HDC activity is another important target for regulation of histamine signaling. It was reported that HDC mRNA, HDC activity, and the histamine content were significantly increased in the nasal mucosa of TDI-sensitized rats (13). These findings suggest that H1R and HDC genes are allergic disease-sensitive genes and the levels of H1R and HDC expression affect the severity of allergic symptoms, and also that compounds that suppress histamine signaling are promising as anti-allergic drugs.…”

Section: A Llergic Rhinitis (Ar)mentioning

confidence: 73%

“…We demonstrated previously that repeated intranasal application of TDI induced release of histamine from mast cells via neurogenic inflammation and led to the development of nasal hypersensitivity in guinea pigs and rats (9 -11). Up-regulation of H1R at both the mRNA and protein level was also observed in the nasal mucosa of TDI-sensitized nasal allergic model rats (12,13). As histidine decarboxylase (HDC) is the only enzyme that synthesizes histamine from histidine in mammals, control of HDC activity is another important target for regulation of histamine signaling.…”

Section: A Llergic Rhinitis (Ar)mentioning

confidence: 99%

“…4c). The histamine content in the nasal mucosa is also reported to increase significantly after TDI provocation with a maximum 9 h after TDI provocation (13). Thus, we next examined the effect of suplatast on the histamine content in the nasal mucosa 9 h after TDI provocation.…”

Section: Effect Of Suplatast On Tdi-induced Increase In the Histaminementioning

confidence: 99%

“…In TDIsensitized rats, HDC mRNA and HDC activity in the nasal mucosa were significantly increased after TDI provocation and reached a maximum 4 and 9 h, respectively, after TDI provocation (13). Thus, we collected nasal mucosa 9 h after TDI provocation and investigated the effect of suplatast on HDC activity.…”

Section: Effect Of Suplatast On Tdi-induced Increases In Hdc Mrna Expmentioning

confidence: 99%

“…Half of supernatant A was dialyzed three times against an adequate volume of HDC buffer for 6 h at 4°C (designated as supernatant B). The histamine content in supernatant A was determined with an HPLC system with cation exchanger (Tosoh) and the automated o-phthalaldehyde fluorometric detection system (Hitachi) by the method described previously (13). HDC activity was determined by incubating supernatant B for 4 h at 37°C with 0.25 mM L-histidine.…”

Section: Measurements Of Hdc Activity and Histamine Content Of Nasal mentioning

confidence: 99%

See 4 more Smart Citations

Suplatast Tosilate Inhibits Histamine Signaling by Direct and Indirect Down-Regulation of Histamine H1 Receptor Gene Expression through Suppression of Histidine Decarboxylase and IL-4 Gene Transcriptions

Shahriar

Mizuguchi

Maeyama

et al. 2009

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

Allergic rhinitis (AR) is an inflammatory disorder typified by symptoms such as sneezing, congestion, and rhinorrhea. Histamine plays important roles in eliciting AR symptoms. Up-regulation of the histamine H1 receptor (H1R) and histidine decarboxylase (HDC) mRNAs was observed in AR patients. Th2 cytokines are also involved in the pathogenesis of AR. We examined the effect of suplatast tosilate on nasal symptoms, and H1R, HDC, and IL-4 gene expression using toluene-2,4-diisocyanate (TDI)-sensitized rats and HeLa cells expressing endogenous H1R. Provocation with TDI increased nasal symptoms, HDC activity, the histamine content of nasal lavage fluid, and the expression of H1R, HDC, and IL-4 mRNAs in TDI-sensitized rats. Pretreatment with suplatast for 2 wk significantly suppressed TDI-induced nasal symptoms and elevation of H1R, HDC, and IL-4 mRNAs. Suplatast also suppressed HDC activity in the nasal mucosa and the histamine content of the nasal lavage fluid. Bilateral injection of IL-4 into the nasal cavity of normal rats up-regulated H1R mRNA, while intranasal application of histamine up-regulated IL-4 mRNA. Suplatast suppressed IL-4-induced up-regulation of H1R mRNA in HeLa cells. However, it did not inhibit histamine-induced H1R mRNA elevation. These results suggest that suplatast alleviates nasal symptoms by inhibiting histamine signaling in TDI-sensitized rats through the suppression of histamine- and IL-4-induced H1R gene expression by the inhibitions of HDC and IL-4 gene transcriptions, respectively.

show abstract

Section: A Llergic Rhinitis (Ar)mentioning

confidence: 73%

Section: A Llergic Rhinitis (Ar)mentioning

confidence: 99%

Section: Effect Of Suplatast On Tdi-induced Increase In the Histaminementioning

confidence: 99%

Section: Effect Of Suplatast On Tdi-induced Increases In Hdc Mrna Expmentioning

confidence: 99%

Section: Measurements Of Hdc Activity and Histamine Content Of Nasal mentioning

confidence: 99%

See 3 more Smart Citations

Suplatast Tosilate Inhibits Histamine Signaling by Direct and Indirect Down-Regulation of Histamine H1 Receptor Gene Expression through Suppression of Histidine Decarboxylase and IL-4 Gene Transcriptions

Shahriar

Mizuguchi

Maeyama

et al. 2009

The Journal of Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

Paeoniflorin inhibits mast cell–mediated allergic inflammation in allergic rhinitis

Wang

Cheng

2018

J of Cellular Biochemistry

View full text Add to dashboard Cite

Paeoniflorin (PF), one of the main effective ingredients from the root of Paeonia lactiflora Pall., was reported to possess antitumor, anti-inflammatory, and antiallergic properties. However, the roles of PF in activated human mast cell line, HMC-1 cells, have not yet been elucidated. Thus, the aim of this study was to examine the antiallergic and anti-inflammatory effects of PF on phorbol-12-myristate 13-acetate plus calcium ionophore (PMACI)-induced human mast cells and to identify the mechanism responsible for these effects. Our results demonstrated that pretreatment with PF effectively attenuated PMACI-induced production of tumor necrosis factor-α and interleukin 1β in HMC-1 cells. In addition, PF significantly suppressed PMACI-induced histamine release and caspase-1 activation in HMC-1 cells. Furthermore, PF prevented the activation of nuclear factor κB (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathways in activated HMC-1 cells. In conclusion, we showed for the first time that PF attenuated the mast cell-mediated allergic inflammatory response through suppressing the NF-κB and MAPK signaling pathways.

show abstract

Interleukin-4 up-regulates histamine H1 receptors by activation of H1 receptor gene transcription

Horio

Fujimoto

Mizuguchi

et al. 2010

Naunyn-Schmied Arch Pharmacol

View full text Add to dashboard Cite

Histamine plays an important role in allergy mainly through histamine H1 receptor (H1R). Recent studies showed that the H1R level is elevated in allergic conditions, suggesting that this will make the allergic symptoms worse by intensifying H1R-mediated processes. Some cytokines are also involved in allergy, and interleukin-4 (IL-4) has been implicated as an important mediator of allergic inflammation. It is noteworthy that the level of IL-4 is elevated under allergic states. We tested whether IL-4 has a role in up-regulating H1R level by using the cultured human HeLa cell as a model system that expresses both IL-4 receptor and H1R. IL-4 stimulation increased H1R protein levels and H1R mRNA levels. IL-4 also increased H1R promoter activity, but had no effect on H1R mRNA stability, indicating that up-regulation of H1R was due to an increase in H1R mRNA synthesis. IL-4 activated STAT6 (signal transducer and activator of transcription 6) in HeLa cells, and up-regulation of H1R mRNA and activation of STAT6 by IL-4 were inhibited by a specific JAK3 (Janus-activated kinase 3) inhibitor. Stimulation with histamine also up-regulated H1R mRNA, and co-stimulation with histamine and IL-4 elevated H1R mRNA level significantly higher than the stimulation with histamine or IL-4 alone did. These results indicated that IL-4 up-regulated H1R mRNA level through increased transcription of H1R gene via JAK3-STAT6 pathway. The effects of histamine and IL-4 were additive, suggesting that these allergic mediators will work together to up-regulate H1R level, and thus make the allergic symptom worse by intensifying H1R-mediated allergic processes.

show abstract

Dexamethasone Suppresses Histamine Synthesis by Repressing both Transcription and Activity of HDC in Allergic Rats

Cited by 27 publications

References 35 publications

Suplatast Tosilate Inhibits Histamine Signaling by Direct and Indirect Down-Regulation of Histamine H1 Receptor Gene Expression through Suppression of Histidine Decarboxylase and IL-4 Gene Transcriptions

Suplatast Tosilate Inhibits Histamine Signaling by Direct and Indirect Down-Regulation of Histamine H1 Receptor Gene Expression through Suppression of Histidine Decarboxylase and IL-4 Gene Transcriptions

Paeoniflorin inhibits mast cell–mediated allergic inflammation in allergic rhinitis

Interleukin-4 up-regulates histamine H1 receptors by activation of H1 receptor gene transcription

Contact Info

Product

Resources

About