Dexamethasone influence on morphine-induced analgesia in outbred Swiss and Inbred DBA/2J and C57BL/6 mice

Capasso, Anna; Giannuario, A. Di; Loizzo, Alberto; Pieretti, Stefano; Sorrentino, L.

doi:10.1016/0278-5846(94)90084-1

Cited by 5 publications

(2 citation statements)

References 31 publications

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“…The choice of adult Swiss mice was guided by the fact that these outbred animals are more sensitive to morphine than inbred ones (Capasso, Di Giannuario, Loizzo, Pieretti, & Sorrentino, 1994). Data were obtained on a Bruker Avance 700 MHz using standard 2D spectra.…”

Section: D Structure Of Cyrtx-1amentioning

confidence: 99%

“…The provider health reports indicated that the animals were free of known viral, bacterial, and parasitic pathogens. The choice of adult Swiss mice was guided by the fact that these outbred animals are more sensitive to morphine than inbred ones (Capasso, Di Giannuario, Loizzo, Pieretti, & Sorrentino, 1994). In addition, taking into account, first, that a clear majority of patients with pain has been reported to be women (Mogil, 2012) and, second, the 3Rs for more ethical use of animals in testing, only female mice were included in our study.…”

Section: D Structure Of Cyrtx-1amentioning

confidence: 99%

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From identification to functional characterization of cyriotoxin‐1a, an antinociceptive toxin from the spider Cyriopagopus schioedtei

Gonçalves

Benoit

Kurz

et al. 2019

British J Pharmacology

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Background and Purpose:The Na V 1.7 channel is highly expressed in dorsal root ganglia of the sensory nervous system and plays a central role in the pain signalling process. We investigated a library prepared from original venoms of 117 different animals to identify new selective inhibitors of this target.Experimental Approach: We used high throughput screening of a large venom collection using automated patch-clamp experiments on human voltage-gated sodium channel subtypes and then in vitro and in vivo electrophysiological experiments to characterize the active peptides that have been purified, sequenced, and chemically synthesized. Analgesic effects were evaluated in vivo in mice models.Key Results: We identified cyriotoxin-1a (CyrTx-1a), a novel peptide isolated from Cyriopagopus schioedtei spider venom, as a candidate for further characterization. This 33 amino acids toxin belongs to the inhibitor cystine knot structural family and inhibits hNa V 1.1-1.3 and 1.6-1.7 channels in the low nanomolar range, compared to the micromolar range for hNa V 1.4-1.5 and 1.8 channels. CyrTx-1a was 920 times more efficient at inhibiting tetrodotoxin (TTX)-sensitive than TTX-resistant sodium currents recorded from adult mouse dorsal root ganglia neurons and in vivo electrophysiological experiments showed that CyrTx-1a was approximately 170 times less efficient than huwentoxin-IV at altering mouse skeletal neuromuscular excitability properties. CyrTx-1a exhibited an analgesic effect in mice by increasing reaction time in the hot-plate assay. Conclusions and Implications:The pharmacological profile of CyrTx-1a paves the way for further molecular engineering aimed to optimize the potential antinociceptive properties of this peptide.

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Section: D Structure Of Cyrtx-1amentioning

confidence: 99%

Section: D Structure Of Cyrtx-1amentioning

confidence: 99%