Dexamethasone and prednisolone treatment in beef cattle: influence on glycogen deposition and gene expression in the liver

Divari, Sara; Lucia, F. De; Berio, Enrica; Sereno, Alessandra; Biolatti, B.; Cannizzo, Francesca Tiziana

doi:10.1016/j.domaniend.2020.106444

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…We found out that OPTN overexpression significantly upregulated the expression FLNA, which was associated with the inhibition of ERS in DCM. Moreover, GSK3β and FoxO1 participate in the occurrence and development of DCM through ERS and glycophagy (Divari et al, 2020; Du et al, 2022; Wu, Lu, et al, 2023; Wu, Xu, et al, 2023). The beneficial effect of OPTN on DCM is likely through regulation of the GSK3β/FoxO1 pathway.…”

Section: Discussionmentioning

confidence: 99%

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

Li,

Yu,

et al. 2024

Phytotherapy Research

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Diabetic cardiomyopathy (DCM) is an important complication resulting in heart failure and death of diabetic patients. However, there is no effective drug for treatments. This study investigated the effect of D‐pinitol (DP) on cardiac injury using diabetic mice and glycosylation injury of cardiomyocytes and its molecular mechanisms. We established the streptozotocin‐induced SAMR1 and SAMP8 mice and DP (150 mg/kg/day) intragastrically and advanced glycation end‐products (AGEs)‐induced H9C2 cells. H9C2 cells were transfected with optineurin (OPTN) siRNA and overexpression plasmids. The metabolic disorder indices, cardiac dysfunction, histopathology, immunofluorescence, western blot, and immunoprecipitation were investigated. Our results showed that DP reduced the blood glucose and AGEs, and increased the expression of heart OPTN in diabetic mice and H9C2 cells, thereby inhibiting the endoplasmic reticulum stress (GRP78, CHOP) and glycophagy (STBD1, GABARAPL1), and alleviating the myocardial apoptosis and fibrosis of DCM. The expression of filamin A as an interaction protein of OPTN downregulated by AGEs decreased OPTN abundance. Moreover, OPTN siRNA increased the expression of GRP78, CHOP, STBD1, and GABARAPL1 and inhibited the expression of GAA via GSK3β phosphorylation and FoxO1. DP may be helpful to treat the onset of DCM. Targeting OPTN with DP could be translated into clinical application in the fighting against DCM.

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Section: Discussionmentioning

confidence: 99%

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

Li,

Yu,

et al. 2024

Phytotherapy Research

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“…For instance, the oral administration of glucocorticoids led to glycogen depletion by either autophagy or inhibition of glycogen synthase. On the other hand, overexpression of genes involved in the gluconeogenic pathway and glycogen synthesis has been reported in domestic animals after dexamethasone administration [ 42 ]. Besides, hepatocyte enlargement along with a decrease in hepatocyte proliferation were recorded after glucocorticoid administration [ 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

Prednisolone and mesenchymal stem cell preloading protect liver cell migration and mitigate extracellular matrix modification in transplanted decellularized rat liver

et al. 2022

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Introduction Regenerative medicine provides promising approaches for treating chronic liver diseases. Previous studies indicate that decellularized liver architecture is damaged by invading non-hepatic inflammatory cells. This study aimed to use anti-inflammatory and regenerative potency of bone marrow-derived mesenchymal stem cells (BM-MSC) and prednisolone for reducing fibrosis and balancing inflammatory cell migration into the decellularized liver scaffold. Material and method The liver was decellularized by perfusing Sodium Lauryl Ether Sulfate (SLES), and nuclei depletion and extracellular matrix (ECM) retention were confirmed by DNA quantification, histochemical, and immunohistochemical assessments. Scaffolds were loaded with BM-MSCs, prednisolone, or a combination of both, implanted at the anatomical place in the rat partial hepatectomized and followed up for 2 and 4 weeks. Results Labeled-MSCs were traced in the transplanted scaffolds; however, they did not migrate into the intact liver. Immunohistochemistry showed that the hepatoblasts, cholangiocytes, stellate, and oval cells invaded into all the scaffolds. Bile ducts were more abundant in the border of the scaffolds and intact liver. Stereological assessments showed a significant reduction in the number of lymphocytes and neutrophils in prednisolone-loaded scaffolds. The regeneration process and angiogenesis were significantly higher in the group treated with cell/prednisolone-loaded bioscaffolds. Collagen fibers were significantly reduced in the scaffolds pre-treated with cell/prednisolone, prednisolone, or BM-MSCs, compared to the control group. Conclusion Loading prednisolone into the scaffolds can be a worthy approach to restrict inflammation after transplantation. Although pre-loading of the scaffolds with a combination of cells/prednisolone could not alleviate inflammation, it played an important role in regeneration and angiogenesis.

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“…The GSK3β gene identified in our study, which is present in several metabolic pathways that influence carcass FTN, was also mentioned by Divari et al . (2020). The authors also identified gene expressions associated with tissues of the liver, the organ partially responsible for the gluconeogenesis process in ruminants.…”

Section: Genomic Regions Gene Symbol Gene Name Var1 (%)mentioning

confidence: 99%

Genome‐wide association study and pathway analysis for carcass fatness in Nellore cattle measured by ultrasound

et al. 2021

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Summary Identifying genes or genomic regions influencing carcass‐quality traits such as fatness (FTN) is essential to optimize the genetic selection processes in beef cattle. The aim of this study was to identify genomic regions associated with FTN in Nellore cattle as well as to elucidate the metabolic pathways related to the phenotypic expression. Ultrasound‐based measurements of FTN were collected in 11 750 animals, with 39 903 animals in the pedigree file. Additionally, 1440 animals were genotyped using the GGP‐indicus 35K SNP panel, which contained 33 623 SNPs after quality control. Twenty genes related to FTN were found on 11 chromosomes, explaining 12.96% of the total additive genetic variance. Gene ontology revealed seven genes: NR1L2, PKD2, GSK3β, EXT1, RAD51B, SORCS1 and DPH6, associated with important processes related to FTN. In addition, novel candidate genes (MAATS1, LYPD1, CDK5RAP2, RAD51B, c13H2Oorf96 and TRAPPC11) were detected and could provide further knowledge to uncover genetic regions associated to carcass fatness in beef cattle.

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Dexamethasone and prednisolone treatment in beef cattle: influence on glycogen deposition and gene expression in the liver

Cited by 8 publications

References 31 publications

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

D‐pinitol alleviates diabetic cardiomyopathy by inhibiting the optineurin‐mediated endoplasmic reticulum stress and glycophagy signaling pathway

Prednisolone and mesenchymal stem cell preloading protect liver cell migration and mitigate extracellular matrix modification in transplanted decellularized rat liver

Genome‐wide association study and pathway analysis for carcass fatness in Nellore cattle measured by ultrasound

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