Dexamethasone acutely suppresses the anabolic SNAT2/SLC38A2 amino acid transporter protein in L6‐G8C5 rat skeletal muscle cells

Blbas, Safia Sabr Ibrahim; Watson, Emma; Butler, Heather; Brown, Jeremy R. Glissen; Herbert, Terence P.; Stover, Cordula M.; Bevington, Alan; Abbasian, Nima

doi:10.1096/fba.2020-00076

Cited by 1 publication

(1 citation statement)

References 57 publications

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“…On the other hand, the L6 myoblasts model is more commonly used for establishment of IR model (Kondo et al, 2021). Dexamethasone was used to induce L6 muscle cells to insulin resistance, and it was also found that dexamethasone would cause Ser307 phosphorylation of insulin receptor substrate after 24 h. Some have also established insulin resistance models in mature L6 cell lines by insulin and palmitate induction (Blbas et al, 2021).…”

Section: Adipocyte Ir Cell Modelmentioning

confidence: 99%

Guidelines for the antidiabetic assay for bioactive substances in cell model

Zhang

Cao

et al. 2022

eFood

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Diabetes is a group of metabolic diseases characterized by hyperglycemia. Insulin resistance (IR) and insufficient insulin secretion are important pathophysiological basis and significant characteristics of diabetes. At present, animal is still the main model for investigation of Antidiabetic activity of bioactive substances, however, in vivo models always has the problems of long experimental cycle, complicated experiment, and high cost. Compared with the animal models, cell culture could avoid some impossible or difficult to eliminate under natural conditions, and observe the experimental results more simply, economically, quickly, and objectively. In this case, the establishment of in vitro diabetic cell model has gradually developed. Recently, cell models are more widely used in highthroughput screening, metabolism analysis, molecular pharmacology investigation, pharmacodynamics evaluation of natural product, cell function, interaction between receptors and cytokines, and so on. These guidelines will point out and discuss to help researchers to choose cell model that are truly reliable and can be successfully applied for their intended investigation of antidiabetic activity.

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Section: Adipocyte Ir Cell Modelmentioning

confidence: 99%