Développement de modèles physiopathologiques cardiaques à partir des cultures de cardiomyocytes

Athias, Pierre; Vandroux, David; Tissier, Cindy; Rochette, Luc

doi:10.1016/j.ancard.2006.02.004

Cited by 9 publications

(1 citation statement)

References 25 publications

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“…Cultured primary cardiomyocytes and human induced pluripotent stem cell derived cardiomyocytes (hiPS-CMs) provided useful models to understand the cardiovascular function and cardiovascular diseases in vitro on real-time cell analysis system (RTCA), which provide a homogeneous population of relatively pure single cells [ 7 ]. This platform has been used for investigation of contraction, hypoxia and cardiotoxicity [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Liensinine- and Neferine-Induced Cardiotoxicity in Primary Neonatal Rat Cardiomyocytes and Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Sun

Wang

et al. 2016

IJMS

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Due to drug-induced potential congestive heart failure and irreversible dilated cardiomyopathies, preclinical evaluation of cardiac dysfunction is important to assess the safety of traditional or novel treatments. The embryos of Nelumbo nucifera Gaertner seeds are a homology of traditional Chinese medicine and food. In this study, we applied the real time cellular analysis (RTCA) Cardio system, which can real-time monitor the contractility of cardiomyocytes (CMs), to evaluate drug safety in rat neonatal CMs and human induced pluripotent stem cell-derived cardiomyocytes (hiPS-CMs). This study showed detailed biomechanical CM contractility in vitro, and provided insights into the cardiac dysfunctions associated with liensinine and neferine treatment. These effects exhibited dose and time-dependent recovery. Neferine showed stronger blocking effect in rat neonatal CMs than liensinine. In addition, the effects of liensinine and neferine were further evaluated on hiPS-CMs. Our study also indicated that both liensinine and neferine can cause disruption of calcium homeostasis. For the first time, we demonstrated the potential cardiac side effects of liensinine or neferine. While the same inhibition was observed on hiPS-CMs, more importantly, this study introduced an efficient and effective approach to evaluate the cardiotoxicity of the existing and novel drug candidates.

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Section: Introductionmentioning

confidence: 99%

Liensinine- and Neferine-Induced Cardiotoxicity in Primary Neonatal Rat Cardiomyocytes and Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Sun

Wang

et al. 2016

IJMS

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The Influence of Sex and Age on Responses of Isolated Ventricular Myocytes to Simulated Ischemia and Reperfusion

Sapp

Howlett

2020

Sex Differences in Heart Disease

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Analysis of an experimental model of in vitro cardiac tissue using phase space reconstruction

Jacquir

Laurent

et al. 2014

Biomedical Signal Processing and Control

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The in vitro cultures of cardiac cells represent valuable models to study the mechanism of the arrhythmias at the cellular level. But the dynamics of these experimental models cannot be characterized precisely, as they include a lot of parameters that depend on experimental conditions. This paper is devoted to the investigation of the dynamics of an in vitro model using a phase space reconstruction. Our model, based on the heart cells of new born rats, generates electrical field potentials acquired using a microelectrode technology, which are analyzed in normal and under external stimulation conditions. Phase space reconstructions of electrical field potential signals in normal and arrhythmic cases are performed after characterizing the nonlinearity of the model, computing the embedding dimension and the time lag. A non-parametric statistical test (Kruskal-Wallis test) shows that the time lag τ could be used as an indicator to detect arrhythmia, while the embedding dimension is not significantly different between the normal and the arrhythmia cases. The phase space reconstructions highlight attractors, whose dimension reveals that they are strange, depicting a deterministic dynamics of chaotic nature in our in vitro model.

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Développement de modèles physiopathologiques cardiaques à partir des cultures de cardiomyocytes

Cited by 9 publications

References 25 publications

Liensinine- and Neferine-Induced Cardiotoxicity in Primary Neonatal Rat Cardiomyocytes and Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Liensinine- and Neferine-Induced Cardiotoxicity in Primary Neonatal Rat Cardiomyocytes and Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

The Influence of Sex and Age on Responses of Isolated Ventricular Myocytes to Simulated Ischemia and Reperfusion

Analysis of an experimental model of in vitro cardiac tissue using phase space reconstruction

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