Developments with Antarctic microorganisms: culture collections, bioactivity screening, taxonomy, PUFA production and cold-adapted enzymes

Nichols, David S.; Bowman, John P.; Sanderson, K; Nichols, Carol Mancuso; Lewis, Thomas E.; McMeekin, TA; Nichols, Peter D.

doi:10.1016/s0958-1669(99)80042-1

Cited by 128 publications

(77 citation statements)

References 59 publications

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“…2). PUFAs appear to be rare in prokaryotes (Nichols et al, 1999;Nichols & McMeekin, 2002): the large amount of PUFAs in SBNa008 T , which can represent nearly 30 % of the total cellular fatty acids, is uncommon for a terrestrial bacterium. However, the occurrence of these fatty acids in SBNa008 T (Table 1) was not surprising, since they have been reported previously in some other novel myxobacteria (Garcia et al, 2009b;Stadler et al, 2010) and some marine genera (Iizuka et al, 2003a, b;Fudou et al, 2002;Garcia et al, 2011).…”

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confidence: 99%

Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium

Garcia

Gemperlein

Müller

2014

International Journal of Systematic and Evolutionary Microbiology

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A bacterial strain designated SBNa008T was isolated from a Philippine soil sample. It exhibited the general characteristics associated with myxobacteria, such as swarming of Gram-negative vegetative rod cells, fruiting body and myxospore formation and predatory behaviour in lysing micro-organisms. The novel strain was characterized as mesophilic, chemoheterotrophic and aerobic. The major fatty acids were C20 : 4ω6,9,12,15 all cis (arachidonic acid), iso-C15 : 0, C17 : 1 2-OH and iso-C15 : 0 dimethylacetal. Interestingly, SBNa008T contained diverse fatty acids belonging to the commercially valuable polyunsaturated omega-6 and omega-3 families, and a highly conjugated dihydroxylated C28 steroid. The G+C content of the genomic DNA was 67.3 mol%. The 16S rRNA gene sequence revealed 95–96 % similarity to sequences derived from clones of uncultured bacteria and 94–95 % similarity to cultured members of the suborder Sorangiineae . Phylogenetic analysis revealed that strain SBNa008T formed a novel lineage in the suborder Sorangiineae . Based on a polyphasic taxonomic characterization, we propose that strain SBNa008T represents a novel genus and species, Minicystis rosea gen. nov., sp. nov. The type strain of Minicystis rosea is SBNa008T ( = DSM 24000T = NCCB 100349T).

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confidence: 99%

Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium

Garcia

Gemperlein

Müller

2014

International Journal of Systematic and Evolutionary Microbiology

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“…Water samples from Ulhas River, Maharashtra were checked for lipid producers as well. Lakes being recognized as sources of PUFA producers by a few researchers previously 8,11 , we have studied water samples from two fresh water lakes from Bengaluru, Karnataka as well; namely Hebbal Lake and Ulsoor Lake. …”

Section: Resultsmentioning

confidence: 99%

“…The isolate GN/PA/HBM11 from Hebbal lake, Bengaluru was seen to be capable of producing Linoleic acid. This is an interesting finding as only a few researchers have considered or reported fresh water sources as a means for isolation of lipid producing microbes 8,11 .…”

Section: Gc/msmentioning

confidence: 93%

“…Increased recognition of the biotechnological potential of microorganisms has occurred over recent years 8 . Screening microbial flora from diverse aquatic sources has become a major thrust area of research for both industry as well as academia 7 .…”

Section: Introductionmentioning

confidence: 99%

“…Water as well as muddy water samples from the Arabian Sea along the coastal regions of Maharashtra particularly the Konkan Strip and Kerala were studied. Owing to the few yet encouraging reports from researchers working on isolation of PUFA producers from fresh water sources; we have also studied water samples from fresh water rivers in India and Lakes in Karnataka for the possibility of PUFA producing microbial inhabitants 4,8,11 .…”

Section: Introductionmentioning

confidence: 99%

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2015

IJPSR

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Polyunsaturated Fatty Acids (PUFA) have been shown to be of major importance in the prevention and treatment of a range of human diseases and disorders. A number of microorganisms have been shown to produce and accumulate PUFA. In the current study we have isolated 86 lipid producing microbes from various sources like a river from northern India, lakes from southern India and marine environments off the western coast of India. The isolates were shortlisted following a preliminary screening scheme by studying a set of parameters like intensity of lipid accumulation as judged by Sudan Black B staining, amount of biomass on nitrogen limited semi-synthetic medium, lipid quantity per gram biomass, relative quantities of saturated and unsaturated fatty acids and reproducibility of results. Lipid sample was extracted and derivatized for GC analysis using standard procedure. Further, GC/MS analysis confirmed that 5 isolates produce PUFA of omega-6(LA) and omega-3(ALA, ETA) type. Selected cultures have been identified by 16S r-RNA sequencing and are being optimized for PUFA production by the technique of fermentation.

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Psychrophilic Bacteria: Isolation and Characterization

Bowman

2003

Encyclopedia of Environmental Microbiology

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Developments with Antarctic microorganisms: culture collections, bioactivity screening, taxonomy, PUFA production and cold-adapted enzymes

Cited by 128 publications

References 59 publications

Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium

Minicystis rosea gen. nov., sp. nov., a polyunsaturated fatty acid-rich and steroid-producing soil myxobacterium

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Psychrophilic Bacteria: Isolation and Characterization

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