Developments of the in-check platform for diagnostic applications

Palmieri, M.; Alessi, Enrico; Conoci, Sabrina; Marchi, M.; Panvini, Gaetano

doi:10.1117/12.778331

Cited by 8 publications

(9 citation statements)

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“…Solid-state (also called solid-phase) microarrays, in particular, use rigid, impermeable substrates (such as glass or silicon wafers) as the solid support (Foglieni et al, 2010;Palmieri, Alessi, Conoci, Marchi, & Panvini, 2008). The microscopic features, each with thousands of identical probes immobilised to the solid surface, are arranged in an orderly fashion at high density.…”

Section: Solid-state Microarraysmentioning

confidence: 99%

Solid and Suspension Microarrays for Microbial Diagnostics

Miller

Karaöz

Brodie

et al. 2015

Methods in Microbiology

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Section: Solid-state Microarraysmentioning

confidence: 99%

Solid and Suspension Microarrays for Microbial Diagnostics

Miller

Karaöz

Brodie

et al. 2015

Methods in Microbiology

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“…Fabrication is performed with a semiconductor-based MEMS process technology, named SIMICH at 8″ wafer diameter, which allows for the fabrication of micro-mechanical, sensor, and microfluidic components. More details on the process steps are available in [120]. Fig.…”

Section: Technology and Operating Principlementioning

confidence: 99%

Diagnostic tools for tackling febrile illness and enhancing patient management

Mitsakakis

D’Acremont

Hin

et al. 2018

Microelectronic Engineering

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A B S T R A C TMost patients with acute infectious diseases develop fever, which is frequently a reason to visit health facilities in resource-limited settings. The symptomatic overlap between febrile diseases impedes their diagnosis on clinical grounds. Therefore, the World Health Organization promotes an integrated management of febrile illness. Along this line, we present an overview of endemic and epidemic etiologies of fever and state-of-the-art diagnostic tools used in the field. It becomes evident that there is an urgent need for the development of novel technologies to fulfill end-users' requirements. This need can be met with point-of-care and near-patient diagnostic platforms, as well as e-Health clinical algorithms, which co-assess test results with key clinical elements and biosensors, assisting clinicians in patient triage and management, thus enhancing disease surveillance and outbreak alerts. This review gives an overview of diagnostic technologies featuring a platform based approach: (i) assay (nucleic acid amplification technologies are examined); (ii) cartridge (microfluidic technologies are presented); (iii) instrument (various detection technologies are discussed); and at the end proposes a way that such technologies can be interfaced with electronic clinical decision-making algorithms towards a broad and complete diagnostic ecosystem.

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“…The miniaturized low-density microarray area of the In-Check LoC contains up to 126 spots, offering the opportunity to design and customize the chip for individual applications. The platform is completed with dedicated software that controls the instruments and runs the data analysis [ 19 , 20 , 21 ]. The miniaturization of the combined PCR-microarray analytical processes performed into a unique biosensor allows different advantages in terms of high sensitivity, simplicity and high-throughput, with short assay time, low reagent consumption, and easiness to automation [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a Pharmacogenetic Lab-on-Chip Assay Based on the In-Check Technology to Screen for Genetic Variations Associated to Adverse Drug Reactions to Common Chemotherapeutic Agents

et al. 2020

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Background: Antineoplastic agents represent the most common class of drugs causing Adverse Drug Reactions (ADRs). Mutant alleles of genes coding for drug-metabolizing enzymes are the best studied individual risk factors for these ADRs. Although the correlation between genetic polymorphisms and ADRs is well-known, pharmacogenetic tests are limited to centralized laboratories with expensive or dedicated instrumentation used by specialized personnel. Nowadays, DNA chips have overcome the major limitations in terms of sensibility, specificity or small molecular detection, allowing the simultaneous detection of several genetic polymorphisms with time and costs-effective advantages. In this work, we describe the design of a novel silicon-based lab-on-chip assay able to perform low-density and high-resolution multi-assay analysis (amplification and hybridization reactions) on the In-Check platform. Methods: The novel lab-on-chip was used to screen 17 allelic variants of three genes associated with adverse reactions to common chemotherapeutic agents: DPYD (Dihydropyrimidine dehydrogenase), MTHFR (5,10-Methylenetetrahydrofolate reductase) and TPMT (Thiopurine S-methyltransferase). Results: Inter- and intra assay variability were performed to assess the specificity and sensibility of the chip. Linear regression was used to assess the optimal hybridization temperature set at 52 °C (R2 ≈ 0.97). Limit of detection was 50 nM. Conclusions: The high performance in terms of sensibility and specificity of this lab-on-chip supports its further translation to clinical diagnostics, where it may effectively promote precision medicine.

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Developments of the in-check platform for diagnostic applications

Cited by 8 publications

References 0 publications

Solid and Suspension Microarrays for Microbial Diagnostics

Solid and Suspension Microarrays for Microbial Diagnostics

Diagnostic tools for tackling febrile illness and enhancing patient management

Development of a Pharmacogenetic Lab-on-Chip Assay Based on the In-Check Technology to Screen for Genetic Variations Associated to Adverse Drug Reactions to Common Chemotherapeutic Agents

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