Developments in the rat adjuvant arthritis model and its use in therapeutic evaluation of novel non-invasive treatment by SOD in Transfersomes

Simões, Sandra; Delgado, Teresa C.; Lopes, Rodrigo J.G.; Jesús, Sara De; Ferreira, A.A.; Morais, José; Cruz, M.E.M.; Corvo, M. Luísa; Martins, M.B.F.

doi:10.1016/j.jconrel.2004.12.008

Cited by 58 publications

(33 citation statements)

References 37 publications

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“…In this model, at least 14-15 days are needed before polyarthritis is reached. The appearance of polyarthritis initiates a secondary stage of the inflammatory response and the lesions in the paws can be seen by radiography on day 21 and day 25 (Noguchi et al, 2005;Simoes et al, 2005). In order to evaluate the therapeutic effect of tripterine on arthritis, tripterine was given to AA rats from day 19 to day 24.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic effect of tripterine on adjuvant arthritis in rats

Zhang

Tan

et al. 2008

Journal of Ethnopharmacology

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a b s t r a c tAims of the study: Tripterygium wilfordii Hoog f., a perennial vine, is used in traditional Chinese medicine for treatment of rheumatoid arthritis. This study was to determine whether tripterine, isolated from Tripterygium wilfordii Hoog f., had therapeutic effects on adjuvant arthritis. Materials and methods: Adjuvant arthritis (AA) was induced in rats on day 0. Tripterine 5, 10 and 20 mg kg −1 day −1 , or prednisone 10 mg kg −1 day −1 was given to rats intragastrically from day 19 to day 24. Results: Tripterine significantly inhibited paw swelling and bone destruction in AA rats. Serum level of IgG anti-Mycobacterium tuberculosis antibodies and delayed-type hypersensitivity (DTH) induced by Mycobacterium tuberculosis were also decreased by tripterine. The effects of tripterine were associated with decreased interleukin-1␤ (IL-1␤) mRNA expression in ankle joint synovial membrane and tumor necrosis factor-␣ (TNF-␣) mRNA expression in homogenized paws from adjuvant-induced arthritic rats. Conclusions: These findings suggested that tripterine had a therapeutic effect on adjuvant arthritis.

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Section: Discussionmentioning

confidence: 99%

Therapeutic effect of tripterine on adjuvant arthritis in rats

Zhang

Tan

et al. 2008

Journal of Ethnopharmacology

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“…3) In contrast, the skin is an available site for topical application of SOD due to its accessibility. Percutaneous delivery of SOD is therefore considered to be a promising method for prophylaxis or therapy of UV-induced skin damage.…”

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confidence: 99%

Efficient Intradermal Delivery of Superoxide Dismutase Using a Combination of Liposomes and Iontophoresis for Protection against UV-Induced Skin Damage

Kigasawa

Miyashita

Kajimoto

et al. 2012

Biological & Pharmaceutical Bulletin

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Superoxide dismutase (SOD) is a potent antioxidant agent that protects against UV-induced skin damage. However, its high molecular weight is a significant obstacle for efficient delivery into the skin through the stratum corneum and development of antioxidant activity. Recently, we developed a non-invasive transfollicular delivery system for macromolecules using a combination of liposomes and iontophoresis, that represents promising technology for enhancing transdermal administration of charged drugs (IJP, 403, 2011, Kajimoto et al.). In this study, in rats we attempted to apply this system to intradermal delivery of SOD for preventing UV-induced skin injury. SOD encapsulating in cationic liposomes was subjected to anodal iontophoresis. After iontophoretic treatment, the liposomes were diffused widely in the viable skin layer around hair follicles. In contrast, passive diffusion failed to transport liposomes efficiently into the skin. Iontophoretic delivery of liposomes encapsulating SOD caused a marked decrease in the production of oxidative products, such as malondialdehyde, hexanoyl lysine, and 8-hydroxi-2-deoxyguanosine, in UV-irradiated skin. These findings suggested that functional SOD can be delivered into the skin using a combination of iontophoresis and a liposomal system. In conclusion, we succeeded in developing an efficient intradermal SOD delivery system, that would be useful for delivery of other macromolecules. Key words superoxide dismutase; iontophoresis; liposome; transfollicular delivery; antioxidant activityThe skin is exposed constitutively to ultraviolet (UV) rays that cause the production of reactive oxygen species (ROS) in the skin, resulting in diverse skin disorders.1) It is well-known that the classes of UV that reach the ground are UVA and UVB. UVA readily penetrates deep layers of the skin, and causes production of melanin and wrinkling associated with destruction of collagen fiber, resulting in aging of the skin. 1)UVB causes skin inflammation and DNA damage that contribute to the pathogenesis of serious skin disorders, such as skin malignancy.1) ROS are also involved in the development of skin damage, and therefore elimination of ROS is essential for protecting the skin from UV-induced damage.Superoxide dismutase (SOD) may be used to prevent and treat ROS-induced disorders of various tissues as it has potent antioxidant activity.2) Because of its poor pharmacokinetic profile, systemic administration of free SOD does not result in induction of efficient in vivo antioxidant activity.3) In contrast, the skin is an available site for topical application of SOD due to its accessibility. Percutaneous delivery of SOD is therefore considered to be a promising method for prophylaxis or therapy of UV-induced skin damage. However, SOD are highly hydrophilic macromolecules and do not readily penetrate the deeper layers of the skin as the outer layer of the skin, the stratum corneum, functions as a significant barrier prohibiting penetration of drug molecules larger than 500 Da. 4)Iontophoresis...

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“…Interactions between liposomes and the stratum corneum have been studied thoroughly and have shown to enable penetration of drugs into the skin. To find out about the factors influencing the efficiency of drug transport, numerous penetration studies have been performed [5][6][7][8][9].…”

Section: Discussionmentioning

confidence: 99%

“…Methods of studying interaction between vesicles and skin include diffusion experiments, visualization by electron microscopy and fluoromicrography [3,4]. To date, the factors influencing the efficiency of drug transport when using liposomes have been investigated in various in vitro skin penetration studies [5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%