Developments in the application of counter-current chromatography to plant analysis

Marston, Andrew; Hostettmann, Kurt

doi:10.1016/j.chroma.2005.10.018

Cited by 249 publications

(142 citation statements)

References 76 publications

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“…As previously reported in the literature [21], the use of solvent systems containing CHCl 3 /MeOH/H 2 O in several proportions does not give satisfying results. Therefore, it was decided to choose different proportions of the system n-hexane/EtOAc/MeOH/H 2 O, which is considered to be a good starting point in liquid -liquid partition [22]. All extractions were repeated in triplicate and then evaluated with the use of HPLC-DAD-ESI-MS analyses.…”

Section: Resultsmentioning

confidence: 99%

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Karioti¹,

Vincieri

Bilia

2009

J of Separation Science

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Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid-liquid extraction and SEC Hypericin and pseudohypericin, the main naphthodianthrones present in Hypericum species are among the most promising natural products, but the research concerning their biological activities is hindered by their low content in the plant. In this paper a method for the rapid isolation of hypericin and pseudohypericin from Hypericum perforatum hydro-alcoholic dried extracts has been developed. Briefly, the method consists of a partition of the extract between organic and aqueous layers and further purification of the richest extract in naphthodianthrones with Sephadex LH-20 column chromatography. A final separation of hypericin from pseudohypericin was achieved using Sephadex LH-60 column chromatography. All partitions were carried out in triplicate and monitored by LC-MS and NMR analyses. The best results were obtained by successive extraction with n-hexane, Et 2 O and EtOAc. A three-step fractionation resulted in 98% content in total naphthodianthrones. To the best of our knowledge this is the first report on the separation of hypericin from pseudohypericin using size exclusion chromatography.

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Section: Resultsmentioning

confidence: 99%

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Karioti¹,

Vincieri

Bilia

2009

J of Separation Science

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“…Numerous applications in the analysis and preparation of natural products by CCC have been reported [1,[36][37][38]. Separation of polyphenolic natural products such as flavonoids are difficult because these compounds tend to show "peak tailing" in RP-HPLC, as well as irreversible adsorption on silica gel.…”

Section: High-speed Hydrodynamic Centrifuges (Hsccc)mentioning

confidence: 99%

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

2009

Chemistry International -- Newsmagazine for IUPAC

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“…HPCCC does not require a solid stationary phase since it relies solely on the partitioning of a sample between the two phases of an immiscible solvent system. When compared with traditional methods of separation, counter-current chromatography demonstrates a number of advantages, including no irreversible adsorption, less expensive solvents, total recovery of the injected sample, a low risk for sample denaturation, and low solvent consumption [8].…”

Section: Introductionmentioning

confidence: 99%

Purification and anticonvulsant activity of xanthotoxin (8-methoxypsoralen)

et al. 2014

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The aim of the present study was to determine the anticonvulsant effects of xanthotoxin (8-methoxypsoralen) isolated from the fruit of Pastinaca sativa L. This plant is used in European traditional medicine, including Poland. For this purpose, high-performance counter-current chromatography was used. Different solvent systems, mixtures of n-heptane, ethyl acetate, methanol and water were tested in order to calculate partition coefficients. Finally, a mixture with the ratio of 1:1:1:1 (v/v/v/v), giving the K value = 0.92, was chosen as optimal. A rapid scale-up process from analytical to preparative was developed. Evaluation of the anticonvulsant action of xanthotoxin in the mouse maximal electroshock-induced seizure test revealed that it produced a clear-cut anticonvulsant action in mice, and the experimentally-derived median effective doses (ED50 values) ranged between 219 and 252 mg kg−1.

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Developments in the application of counter-current chromatography to plant analysis

Cited by 249 publications

References 76 publications

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Rapid and efficient purification of naphthodianthrones from St. John's wort extract by using liquid–liquid extraction and SEC

Countercurrent Chromatography in Analytical Chemistry (IUPAC Technical Report)

Purification and anticonvulsant activity of xanthotoxin (8-methoxypsoralen)

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