Developments in purification methods for obtaining and evaluation of collagen derived endogenous angioinhibitors

Abstract

Collagen constitutes one of the vital components of the basement membrane scaffolds. Non-collagenous domains (NC1) derived from collagens exhibit potent anti-angiogenic properties, thus attaining significance in regulation of angiogenesis promoted diseases. Individual NC1 domains essential for anti-angiogenic evaluations are generally obtained through purification of individual non-collagenous domains, which have undergone steady developments for enhancing the yields, purpose of biological evaluations and solubility based on the nature of different NC1 domains. This review focuses on the method developments in obtaining biologically active NC1 domains and for specific evaluations in different scenarios.

The role of angiogenesis in implant dentistry part II: The effect of bone-grafting and barrier membrane materials on angiogenesis

“…Gunda et al indicated that collagen has a component known as non-collagenous domains (NC1), which imposes anti-angiogenic effects on surrounding tissues ( 61 ). Shen et al reported that prolyl hydroxylase enzyme (PHD) is a key enzyme with central role in degradation of hypoxia inducible factor alpha (HIF-1α), which is an angiogenic initiating factor in tissue development, regenerations, and reparations ( 62 ).…”

Elastin Barrier Membranes for Guided Tissue Regeneration Technologies

“…Mature vascular networks successfully formed during regeneration can promote and accelerate regenerative processes [7]. The induction of both pro-angiogenic and antianiogenic factors has been shown for collagen membranes, while which of them prevail and at which stages of the inflammatory-regenerative process is still unknown [9,10]. To enhance the proangiogenic effect, collagen membranes can be additionally saturated with VEGF, however, the kinetics of the release of molecules from the material remains uncontrolled and does not always lead to a successful result.…”