Developments in mycotoxin analysis: an update for 2014-2015

Berthiller, Franz; Brera, Carlo; Crews, Colin; Iha, Maria Helena; Krska, Rudolf; Lattanzio, Veronica M.T.; MacDonald, Susan; Malone, R.J.; Maragos, Chris M.; Solfrizzo, Michele; Stroka, Joerg; Whitaker, T. B.

doi:10.3920/wmj2015.1998

Cited by 53 publications

(39 citation statements)

References 128 publications

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“…Five AOH conjugates were isolated and identified by MS and NMR spectroscopy as -D-glucosides (attached in AOH 3-or 9-position). For AME, conjugation resulted in -D-glucoside (mainly attached in the AME 3-position) [52]. Knowledge of the toxicity and disposition of the oxidative AME and AOH metabolites is mandatory for a better understanding of the health risks posed by these Alternaria toxins, since toxicity properties may be enhanced or attenuated for their metabolites [53].…”

Section: Stability Of Alternaria Mycotoxins the Stability Ofmentioning

confidence: 99%

AlternariaMycotoxins in Food and Feed: An Overview

Escrivá

Oueslati

Font

et al. 2017

Journal of Food Quality

140

127

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Alternaria is one of the major mycotoxigenic fungal genera with more than 70 reported metabolites. Alternaria mycotoxins showed notably toxicity, such as mutagenicity, carcinogenicity, induction of DNA strand break, sphingolipid metabolism disruption, or inhibition of enzymes activity and photophosphorylation. This review reports on the toxicity, stability, metabolism, current analytical methods, and prevalence of Alternaria mycotoxins in food and feed through the most recent published research. Half of the publications were focused on fruits, vegetables, and derived products-mainly tomato and apples-while cereals and cereal byproducts represented 38%. The most studied compounds were alternariol, alternariol methyl ether, tentoxin, and tenuazonic acid, but altenuene, altertoxins (I, II, and III), and macrosporin have been gaining importance in recent years. Solid-liquid extraction (50%) with acetonitrile or ethyl acetate was the most common extraction methodology, followed by QuEChERS and dilutiondirect injection (both 14%). High-and ultraperformance liquid chromatography coupled with tandem mass spectrometry was the predominant determination technique (80%). The highest levels of alternariol and alternariol methyl ether were found in lentils, oilseeds, tomatoes, carrots, juices, wines, and cereals. Tenuazonic acid highest levels were detected in cereals followed by beer, while alternariol, alternariol methyl ether, tenuazonic acid, and tentoxin were found in legumes, nuts, and oilseeds.

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Section: Stability Of Alternaria Mycotoxins the Stability Ofmentioning

confidence: 99%

AlternariaMycotoxins in Food and Feed: An Overview

Escrivá

Oueslati

Font

et al. 2017

Journal of Food Quality

140

127

View full text Add to dashboard Cite

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“…Moreover, new developments and updates in this field are covered on a yearly basis in World Mycotoxin Journal [25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Malachová

Stranska-Zachariasova

Václavíková

et al. 2017

Anal Bioanal Chem

125

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Liquid chromatography (LC) coupled with mass spectrometry (MS) is widely used for the determination of mycotoxins in cereals and cereal-based products. In addition to the regulated mycotoxins, for which official control is required, LC-MS is often used for the screening of a large range of mycotoxins and/or for the identification and characterization of novel metabolites. This review provides insight into the LC-MS methods used for the determination of co-occurring mycotoxins with special emphasis on multiple-analyte applications. The first part of the review is focused on targeted LC-MS approaches using cleanup methods such as solid-phase extraction and immunoaffinity chromatography, as well as on methods based on minimum cleanup (quick, easy, cheap, effective, rugged, and safe; QuEChERS) and dilute and shoot. The second part of the review deals with the untargeted determination of mycotoxins by LC coupled with high-resolution MS, which includes also metabolomics techniques to study the fate of mycotoxins in plants.

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“…In order to prevent AF-associated health risks, maximum allowable AF limits for food and feedstuff are regulated [18,19]. The AF analysis systems accepted by the regulations include instrumental analysis with HPLC or LC–MS/MS; and immunological test methods including ELISA [20,21]. …”

Section: Introductionmentioning

confidence: 99%

Aflatoxin-specific Monoclonal Antibody Selection for Immunoaffinity Column Development

et al. 2019

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Antibodies are the basic components of immunoanalytical systems used for detection of a wide range of analytes. Although there are some ground rules for antibody selection, analyte- and assay-specific criteria are the ones that determine the ultimate success of the immunoassays. In this study, we introduced an effective antibody selection procedure for the development of immunoaffinity columns for aflatoxins. The designed scheme puts emphasis on solvent- and matrix-related characterization steps and was used to comparatively evaluate eight monoclonal antibodies. The selected antibody was tolerant to 40% methanol, 20% acetonitrile, 30% acetone and 40% ethanol and did not interact with corn, red pepper or hazelnut extracts. Immunoaffinity columns developed with the selected antibody were validated by 15 independent aflatoxin analysis laboratories.

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Developments in mycotoxin analysis: an update for 2014-2015

Cited by 53 publications

References 128 publications

AlternariaMycotoxins in Food and Feed: An Overview

AlternariaMycotoxins in Food and Feed: An Overview

Advanced LC–MS-based methods to study the co-occurrence and metabolization of multiple mycotoxins in cereals and cereal-based food

Aflatoxin-specific Monoclonal Antibody Selection for Immunoaffinity Column Development

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