Developments in coupled solid‐phase extraction–capillary electrophoresis 2011–2013

Ramautar, Rawi; Somsen, Govert W.; Jong, Gerhardus J. de

doi:10.1002/elps.201300335

Cited by 55 publications

(44 citation statements)

References 53 publications

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“…As the SPE column is an integrated part of the CE system in an in‐line SPE‐CE approach, the separation voltage is also applied across the SPE material during electrophoretic separations. Until now, packed beds have most often been used for the design of in‐line SPE‐CE systems . Next to the design of in‐line SPE‐CE systems, optimization of SPE parameters is a critical step in method development.…”

Section: Introductionmentioning

confidence: 99%

Developments in coupled solid‐phase extraction–capillary electrophoresis 2013–2015

2015

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An overview of the design and application of coupled solid-phase extraction-capillary electrophoresis (SPE-CE) systems reported in the literature between July 2013 and June 2015 is provided in this paper. The present article is a continuation of our previous review papers on this topic which covered the time period 2000-2013 (Electrophoresis 2008, 29, 108-128; Electrophoresis 2010, 31, 44-54; Electrophoresis 2012, 33, 243-250; Electrophoresis 2014, 35, 128-137). The use of in-line and on-line SPE-CE approaches is treated and outlined in this review. Recent advancements, such as, for example, the use of aptamers as affinity material for in-line SPE-CE, the use of a bead string design for in-line fritless SPE-CE, and new interfacing techniques for the on-line coupling of SPE to CE, are outlined. Selected examples demonstrate the applicability of the coupled SPE-CE systems for biomedical, pharmaceutical, environmental, and food studies. A complete overview of the recent SPE-CE studies is given in table format, providing information on sample type, SPE sorbent, coupling mode, detection mode, and LOD. Finally, some general conclusions and perspectives are provided.

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Section: Introductionmentioning

confidence: 99%

Developments in coupled solid‐phase extraction–capillary electrophoresis 2013–2015

2015

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“…According to the focusing mechanism, on‐line sample pre‐concentration strategies can be grouped into four major principles: (i) sample stacking, (ii) sample sweeping, (iii) pH junction and (iv) transient ITP. Samples can also be concentrated using the classical SPE methods known from chromatography in off‐line or on‐line arrangement . But a number of these methods have limitations, mainly due to their specific demands for sample composition.…”

Section: Introductionmentioning

confidence: 99%

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Glatz

2014

Electrophoresis

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Separation technologies play an important role in revealing biological processes at various omic levels, in pharmacological and clinical research. In this context, CE is a strong candidate for analyses of samples with rapidly increasing complexity. Even though CE is well known for its many advantages in this regard, the sensitivity of CE analyses is insufficient for many applications. Accordingly, there are generally three main options for enhancing the sensitivity of CE analyses - using special detection techniques, using sample pre-concentration and derivatisation. Derivatisation is often the method of choice for many laboratories, since it is simple and provides several advantages such as small sample volume demand and the possibility of automation. Although it can be performed in different ways depending on where the reaction takes place, this article reviews one of the simplest and at the same time most useful approaches on-capillary derivatisation. Even if in many cases the use of on-capillary derivatisation alone is enough to improve the detection sensitivity, on other occasions it needs to be employed in combination with the other above-mentioned strategies. After a simple discussion of derivatisation in general, special attention is focused on the on-capillary approach and methodologies available for on-capillary reactant mixing. Its applications in various fields are also described.

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“…Interesting details on SPE extraction procedures prior to CE‐MS analysis can be found in a recent review article by Ramautar et al. .…”

Section: Analysis Of Human Fluids Collected By Noninvasive Methodsmentioning

confidence: 99%

Recent applications of CE‐ and HPLC‐MS in the analysis of human fluids

et al. 2015

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The present review intends to cover the literature on the use of CE-/LC-MS for the analysis of human fluids, from 2010 until present. It has been planned to provide an overview of the most recent practical applications of these techniques to less extensively used human body fluids, including, bronchoalveolar lavage fluid, synovial fluid, nipple aspirate, tear fluid, breast fluid, amniotic fluid, and cerumen. Potential pitfalls related to fluid collection and sample preparation, with particular attention to sample clean-up procedures, and methods of analysis, from the research laboratory to a clinical setting will also be addressed. While being apparent that proteomics/metabolomics represent the most prominent approaches for global identification/quantification of putative biomarkers for a variety of human diseases, evidence is also provided of the suitability of these sophisticated techniques for the detection of heterogeneous components carried by these fluids.

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Developments in coupled solid‐phase extraction–capillary electrophoresis 2011–2013

Cited by 55 publications

References 53 publications

Developments in coupled solid‐phase extraction–capillary electrophoresis 2013–2015

Developments in coupled solid‐phase extraction–capillary electrophoresis 2013–2015

On‐capillary derivatisation as an approach to enhancing sensitivity in capillary electrophoresis

Recent applications of CE‐ and HPLC‐MS in the analysis of human fluids

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