Developmentally regulated biosynthesis of two male specific serum polypeptides in the fat body of the medfly Ceratitis capitata

Characterization of an allatotropin-like peptide receptor in the red flour beetle, Tribolium castaneum

“…However, also the male fat body contains male specific proteins (Thymianou et al, 1995) and expresses male courtship genes (Lazareva et al, 2007). This male-biased expression could indicate that the male fat body plays a role in reproduction.…”

Medfly promoters relevant to the sterile insect technique

“…In a2PS lines, X-gal staining was detected approximately 72 h after eclosion whereas in a2PL lines within the first 30 h after eclosion. Relative to the endogenous MSSP expression, which starts 24 h after eclosion (Thymianou et al, 1995), the expression of the transgene was delayed by 50 h in a2PS lines but correct in a2PL lines. Quantitative measurements of the b-galactosidase activity and western analysis during adult development, in a a2PL line, showed that the expression pattern of the transgenic protein was very similar to that of the endogenous protein.…”

“…Five male-specific serum proteins (MSSPs) have been characterized in the medfly (Katsoris et al, 1990;Thymianou et al, 1995). The two major ones are homodimers of two related polypeptides (MSSP-a and -b), with molecular weights of 14.5 and 13.5 kDa respectively, while the others are homo-and hetero-dimers of a-and b-type polypeptides.…”

Transcriptome analysis in Ceratitis capitata to unveil genes involved in ageing-maturation process

“…The mssp gene family in medfly consists of seven members classified in three subgroups according to the degree of the deduced polypeptide similarity: two mssp α, three mssp β, and two mssp γ. Five mssps have been characterized in the medfly (Katsoris et al, 1990;Thymianou et al, 1995). The synthesis of the mssps has been described to take place mainly during the first 5 days after adults' emergence (Thymianou et al, 1998;Scolari et al, 2012).…”