Developmental validation studies on the RapidHIT™ Human DNA Identification System

Evaluation of the RapidHIT™ 200 System: A comparative study of its performance with Maxwell ® DNA IQ™/Identifiler ® Plus/ABI 3500xL workflow

“…These are, however, attempts which accelerate individual steps of the DNA analysis process. The RapidHIT TM 200 from IntegenX Inc., in contrast, offers an integrated single-instrument platform that combines sample DNA extraction, STR-PCR amplification, amplicon separation and detection, and DNA profile analysis, all within a relatively short duration of about 2 h [16][17][18][19][20][21][22].…”

“…The instrument has been manufacturervalidated [16][17][18] and tested by various groups [19][20][21][22] for the processing of reference buccal samples. In the present study, we seek to evaluate the performance of the RapidHIT TM 200 to generate DNA profiles from samples routinely encountered in crime casework.…”

Integrated Polymerase Chain Reaction Technologies (Sample-to-Answer Technologies)

“…The RapidHIT Ô 200 System ( Fig. 5.2) provides an illustration of this more complex workflow (Holland and Wendt, 2015;Hennessy et al, 2013). The RapidHIT Ô 200 is a fully integrated sample-to-profile DNA identification system that uses four disposable cartridges in conjunction with an instrument base unit.…”

Assessing the impact of common forensic presumptive tests on the ability to obtain results using a novel rapid DNA platform

“…Recently, there have been a number of rapid DNA profiling and detection platforms released that allow the analyses and prioritisation of items by non-expert users outside the traditional laboratory setting [5][6][7][8][9]. The development and release of novel technology into the forensic arena is both carefully controlled and monitored [10].…”

“…For example, all rapid DNA systems look to combine the three current stand-alone processes of DNA extraction, PCR and STR detection steps [e.g. [5][6][7][8][9]. The move out of the laboratory brings additional risks as samples get processed earlier after collection and the boundaries between forensic processes begin to merge [10].…”