Developmental validation of the PowerPlex® 16 HS System: An improved 16-locus fluorescent STR multiplex

Ensenberger, Martin G.; Thompson, Jonelle M.; Hill, B Mueller; Homick, K.M.; Kearney, Veronica F.; Mayntz-Press, Kathleen A.; Mazur, Paul M.; McGuckian, Amy; Myers, Jelena; Raley, Kelli; Raley, Stewart G.; Rothove, Robin; Wilson, Jonathan P.; Wieczorek, Doug; Fulmer, Patricia M.; Storts, Douglas R.; Krenke, Benjamin E.

doi:10.1016/j.fsigen.2009.10.007

Cited by 79 publications

(50 citation statements)

References 11 publications

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“…The consistency of typing results was evaluated considering previously typing through already validated kits such as AmpF l STR ® Identifiler Plus (7), AmpF l STR ® NGM PCR kits (Life Technologies, Carlsbad, CA, USA), PowerPlex ® 16 HS System (8) or PowerPlex ® ESX 17 System (9) (Promega, Madison, WI, USA). DNA concentration was determined by measuring the absorbance of the sample at 260 nm, using the spectrophotometer NanoDrop 1000 (ThermoScientific, Waltham, Massachusetts, USA).…”

Section: Methodsmentioning

confidence: 99%

PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples

Cisana

Cerri

Bosetti³

et al. 2017

Croat Med J

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AimTo report on the successful analysis of amplicons obtained with PowerPlex® Fusion 6C System, a highly robust 27-plex genotyping kit developed for human identification laboratories, on the Applied Biosystems® 3500 Genetic Analyzer.MethodWe performed characterization and evaluation studies following the Scientific Working Group on DNA Analysis Methods (SWGDAM) validation guidelines, examining several critical areas of kit performance. We report the results of sensitivity, robustness, heterozygous peak height ratio, precision, concordance, caseworks, and mixture interpretations. We tested sensitivity, using serial dilutions of control DNA.ResultsThe minimum amount of input DNA resulting in a full profile was 125 pg. Inhibition, inducted by urea, showed a progressively fragmentation of DNA and a full profile was obtained until 1M of inhibitor factor. To test the profile quality, casework samples were extracted with different extraction methods: Chelex®100, QIAmp DNA Micro Kit and Phenol-Chloroform extraction. The results demonstrated that extraction chemistries do not have affect on amplification performance. Concordance check was performed by typing some casework samples and comparing the typing results with those obtained with other available kits. Thus, concordance was expected and supported by the data.ConclusionReliable DNA typing results can be obtained using this new kit, demonstrating its effectiveness and utility in forensic analysis.

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Section: Methodsmentioning

confidence: 99%

PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples

Cisana

Cerri

Bosetti³

et al. 2017

Croat Med J

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“…Two simulated reference control samples M and O were obtained from swabbing the inside of the cheek of two individuals and transferred to FTA® paper. Samples M and O were also extracted using phenol‐chloroform‐isoamyl alcohol and amplified using PowerPlex16 HS (Promega) under the manufacturer's standard conditions and used as comparison samples to test extraction efficiency . A small reference population was also obtained from 18 individuals by swabbing the inside of their cheeks and transferring the swabbing to the FTA® paper; these samples were then analyzed.…”

Section: Methodsmentioning

confidence: 99%

Rapid direct PCR for forensic genotyping in under 25 min

Aboud

McCord

2013

Electrophoresis

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In this paper, a rapid thermal cycling procedure is combined with a direct amplification from a paper punch, permitting a high-speed amplification of a 7-locus multiplex that requires no extraction step. When coupled with a short 1.8 cm microfluidic electrophoresis system, the entire procedure from paper punch to genotype can be completed in under 25 min. The paper describes selection and optimization of enzyme, direct amplification conditions, the reproducibility of the procedure, and concordance with standard forensic genotyping methods. The procedure utilizes a small high-speed thermal cycler and microfluidic device along with a small laptop and is highly portable. Overall, this technique should provide a useful and reliable procedure for rapid determination of identity of individuals retained at checkpoints as well as a quick method for preliminary identification of individuals at remote locations following mass disasters.

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“…These analyses are conducted through the use of commercially-available kits, such as Identifiler 1 Plus (Applied Biosystems 1 , Foster City, CA) and the PowerPlex 1 16HS System (Promega Corporation, Madison, WI). These kits are designed to co-amplify targets of 15 polymorphic STR loci plus Amelogenin, a sex determining marker, and contain advanced buffering components designed to produce results in the presence of polymerase chain reaction (PCR) inhibitors [1,2]. Optimal performance of these kits requires adding DNA template to PCR reagents within the working range of the kit.…”

Section: Introductionmentioning

confidence: 99%

“…Taking into consideration the potential challenges of attempting to quantify inhibited DNA samples, two questions are addressed: (1) Are DNA samples that include metal ions accurately quantified? and, (2) Does the IPC detect the presence of metal ion PCR inhibitors? Because metal ions have the potential to interact directly with DNA and inhibit PCR, it is important to elucidate the reliability of results obtained from PCR-based methods in their presence and the ability of the IPC to differentiate a true negative from an inhibited sample.…”

Section: Introductionmentioning

confidence: 99%

The effects of metal ion PCR inhibitors on results obtained with the Quantifiler ® Human DNA Quantification Kit

Combs

Warren

Huynh

et al. 2015

Forensic Science International: Genetics

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Developmental validation of the PowerPlex® 16 HS System: An improved 16-locus fluorescent STR multiplex

Cited by 79 publications

References 11 publications

PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples

PowerPlex® Fusion 6C System: evaluation study for analysis of casework and database samples

Rapid direct PCR for forensic genotyping in under 25 min

The effects of metal ion PCR inhibitors on results obtained with the Quantifiler ® Human DNA Quantification Kit

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