Developmental validation of a novel six-dye typing system with 47 A-InDels and 2 Y-InDels

Chen, Ling; Du, Weian; Wu, Weibin; Yu, Anlu; Pan, Xiyong; Feng, Peipei; Feng, Chunlei; Li, Chen; Xu, Lingdan; Liu, Changhui; Liu, Chao

doi:10.1016/j.fsigen.2019.02.009

Cited by 40 publications

(36 citation statements)

References 27 publications

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“…The allele frequency differences between Asian, European, and American populations are valuable in understanding the population genetic dynamics and useful in forensic identifications. Thus, we can select different loci for various forensic applications and design suitable forensic kit for the corresponding populations (Chen et al, 2019;Martinez et al, 2019)…”

Section: Linkage Disequilibrium Forensic Parameters and Allele Freqmentioning

confidence: 99%

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Forensic Features and Population Genetic Structure of Dong, Yi, Han, and Chuanqing Human Populations in Southwest China Inferred From Insertion/Deletion Markers

Liu

Zhang

et al. 2020

Front. Genet.

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Guizhou province in southwest China has abundant genetic and cultural diversities, but the forensic features and genetic structure of Guizhou populations remain poorly understood due to the sparse sampling of present-day populations. Here, we present 30 insertion/deletion polymorphisms (InDels) data of 591 human individuals collected from four populations, Dong, Yi, Han, and Chuanqing residing in Guizhou. We calculated the forensic parameters of 30 InDel loci and found that this panel meets the efficiency of forensic personal identification based on the high combined power of discrimination, but it could only be used as a complementary tool in the parentage testing because of the lower combined probability of exclusion values. The studied populations are genetically closer related to geographically adjacent or linguistically related populations in southern China, such as the Tai-Kadai and Hmong-Mien speaking groups. The unrecognized ethnic Chuanqing people show an additional genetic affinity with Han Chinese, highlighting the role of possible military immigrations in their origin.

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Section: Linkage Disequilibrium Forensic Parameters and Allele Freqmentioning

confidence: 99%

“…In addition, InDels can be analyzed through simple PCR amplification and high-throughput electrophoresis, which are commonly used in forensic short tandem repeat (STR) analysis. Therefore, InDels have been increasingly explored and used in forensic genetics (Arenas et al, 2017;Chen et al, 2019;Pan et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Forensic Features and Population Genetic Structure of Dong, Yi, Han, and Chuanqing Human Populations in Southwest China Inferred From Insertion/Deletion Markers

Liu

Zhang

et al. 2020

Front. Genet.

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“…Moreover, variabilities of the alleles for some STR markers were screened from previously published studies. Allelic ladder was generated according to previous reports (Chen et al, 2019).…”

Section: Allelic Ladder Constructionmentioning

confidence: 99%

Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species

et al. 2020

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Species identification of unknown biological samples is of fundamental importance for forensic applications, especially in crime detection, poaching, and illegal trade of endangered animals as well as meat fraud. In this study, a novel panel was developed to simultaneously identify 10 different animal species (Gallus domesticus, Anas platyrhynchos domesticus, Ovis aries, Sus scrofa domesticus, Bos taurus, Equus caballus, Columba livia domestica, Rattus norvegicus, Mus musculus, and Canis lupus familiaris) and human beings by amplifying 22 short tandem repeat (STR) loci in a multiplex PCR using a set of five fluorescently labeled dyes. This novel 22-STR panel was validated by optimization of PCR conditions as well as species specificity, sensitivity, reproducibility, precision, DNA mixture, and tissue/organ consistency. The results of developmental validation showed that the 22-STR loci achieved high species specificity among 10 animal species and human beings, and the sensitivity of this panel was 0.09 ng. This 22-STR panel identified different meats in mixed samples, and the minimum detected mixture ratio in the current test was 10% (0.1 ng/1 ng). This sensitive, accurate, and specific 22-STR panel can be used for forensic species identification and the detection of meat fraud and adulteration.

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“…More importantly, DNA genotypes of DIP loci can be easily detected by capillary electrophoresis. Recently, forensic researchers developed some DIP panels for human identifications [15,16] or ancestry analyses [17,18]. To further enhance the discrimination power of DIP loci, Wendt et al assessed genetic variations in the flanking regions of 68 DIP loci by using the next generation sequencing (NGS) and found that these adjacent variations could improve forensic efficiency of the single DIP locus [14].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Developing and population analysis of a new multiplex panel of 18 microhaplotypes and compound markers using next generation sequencing and its application in the Shaanxi Han population

et al. 2020

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Compound marker consists of two different types of genetic markers, like deletion/insertion polymorphism and single nucleotide polymorphism in the genomic region of 200 bp, and microhaplotype consists of a series of closely linked single nucleotide polymorphisms in a small DNA segment (<300 bp), which show great potential for human identifications and mixture analyses. In this study, we initially selected 23 novel genetic markers comprising 10 microhaplotypes and 13 compound markers according to previously reported single nucleotide polymorphism or deletion/insertion polymorphism loci. Genetic distributions of these 23 loci in different continental populations showed that they could be used as valuable loci for forensic human identification purpose. Besides, high informativeness values (>0.1) were observed in six loci which could be further employed for forensic ancestry analyses. Finally, 18 loci were successfully developed into a multiplex panel and detected by the next generation sequencing (NGS) technology. Further analyses of these 18 loci in the studied Shaanxi Han population showed that 15 loci exhibited relatively high expected heterozygosities (>0.5). Cumulative power of discrimination (0.999 999 999 99 4835) of these 18 loci revealed that the multiplex panel could also be utilized for human identifications in the studied Shaanxi Han population.

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Developmental validation of a novel six-dye typing system with 47 A-InDels and 2 Y-InDels

Cited by 40 publications

References 27 publications

Forensic Features and Population Genetic Structure of Dong, Yi, Han, and Chuanqing Human Populations in Southwest China Inferred From Insertion/Deletion Markers

Forensic Features and Population Genetic Structure of Dong, Yi, Han, and Chuanqing Human Populations in Southwest China Inferred From Insertion/Deletion Markers

Development and Validation of a Novel Five-Dye Short Tandem Repeat Panel for Forensic Identification of 11 Species

Developing and population analysis of a new multiplex panel of 18 microhaplotypes and compound markers using next generation sequencing and its application in the Shaanxi Han population

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