Developmental toxicity of 2,4-dichlorophenoxyacetic acid in zebrafish embryos

Li, Kang; Wu, Jiaqi; Jiang, Lingling; Shen, Lizhen; Li, Jianying; He, Zhiheng; Wei, Ping; Lv, Zhuo; He, Ming-Fang

doi:10.1016/j.chemosphere.2016.12.032

Cited by 125 publications

(46 citation statements)

References 45 publications

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“…Fish exposed to 2,4‐D have been shown to have increased oxidative stress in the kidneys, liver, and brain (Ozcan Oruc et al ; Atamaniuk et al ). Li et al () reported that zebrafish embryos exposed to 25 ppm 2,4‐D showed a decreased hatch rate and survival, increased manifestation of pericardial edemas, and impacted regulation of key heart and oxidative stress biomarker genes. Although Li et al () used exposure concentrations above those of the present study and a different model aquatic vertebrate, their report provides insight into how chronic low‐concentration exposures may be acting to induce the significant decreases in larval survival observed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Effects of low, subchronic exposure of 2,4‐Dichlorophenoxyacetic acid (2,4‐D) and commercial 2,4‐D formulations on early life stages of fathead minnows (Pimephales promelas)

Dehnert

Freitas

DeQuattro

et al. 2018

Enviro Toxic and Chemistry

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Aquatic herbicides are commonly used to control a wide variety of algae and plants, but they also have the potential to contaminate and affect nontarget organisms. However, the impacts of low-level 2,4-dichlorophenoxyacetic acid (2,4-D) herbicide exposure on larval fish are not well understood. We conducted a series of experiments to determine the effects of low concentrations (0.05, 0.50, and 2.00 ppm) of 2 commercial 2,4-D amine salt herbicide formulations (Weedestroy® AM40 [WAM40] and DMA® 4 IVM [DMA4]) and pure 2,4-D on the development and survival of fathead minnows (Pimephales promelas) at various life cycle stages. Larval survival (30 d post hatch [dph]) was decreased following exposure of eggs and larvae to pure 2,4-D (0.50 ppm; p ≤ 0.001), as well as to WAM40 (0.50 and 2.00 ppm; p ≤ 0.001, p ≤ 0.001) and DMA4 (0.50 and 2.00 ppm; p ≤ 0.001, p ≤ 0.001). The results also narrowed the critical window of exposure for effects on survival to the period between fertilization and 14 dph. Development was not negatively altered by any of the compounds tested, although the commercial formulations increased larval total length and mass at 2.00 ppm. Altogether, the results indicate that the use of 2,4-D herbicides for weed control in aquatic ecosystems at current recommended concentrations (<2 ppm whole lake; <4 ppm spot treatment) could present risks to fathead minnow larval survival. Environ Toxicol Chem 2018;37:2550-2559. © 2018 SETAC.

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Section: Discussionmentioning

confidence: 99%

Effects of low, subchronic exposure of 2,4‐Dichlorophenoxyacetic acid (2,4‐D) and commercial 2,4‐D formulations on early life stages of fathead minnows (Pimephales promelas)

Dehnert

Freitas

DeQuattro

et al. 2018

Enviro Toxic and Chemistry

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“…Owing to factors like low costs, short experimental periods (4-7 days), no evident immune system reactions, and clearly recognized transplanted-tumor cells [19], the zebrafish model-as a developing transplantation tumor model-has attracted our attention in recent years. Based on our recent experience with in vivo toxicology and anti-tumor activity studies using the zebrafish model [18], we finally selected this model as a platform to determine the in vivo effects of LC against EC.…”

Section: Discussionmentioning

confidence: 99%

“…Wild-type zebrafish (Danio rerio; Tubingen line) were obtained from Model Animal Research Center of Nanjing University and were kept at 28.5 °C as described previously [18]. Fertilized zebrafish eggs of the transgenic strain expressing green fluorescent protein (GFP) under the flk-1 promoter (flk-1: GFP) were used for in vivo imaging as previously described [19].…”

Section: In Vivo Analysismentioning

confidence: 99%

Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway

Gong

Zhou

et al. 2018

Cell Physiol Biochem

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Background/Aims: Emerging evidence suggests that curcumin possesses chemopreventive properties against various cancers. However, its poor bioavailability limits its clinical application. In this study, we aimed to utilize encapsulation in liposomes (Lipo) as a strategy for the clinical administration of curcumin for endometrial carcinoma (EC). Methods: Curcumin was encapsulated in a liposomal delivery system to prepare a formulation of liposomal curcumin (LC). EC cell lines Ishikawa and HEC-1 were treated with the compound and cell proliferation was measured using MTT assay. Hoechst 33258 staining assay and flow cytometry were used to detect apoptosis of the cells. Wound healing and cell invasion assays were employed to monitor cell motility. Underlying target signaling, such as NF-κB, caspases, and MMPs, were further studied via qRT-PCR and western blot. Thereafter, a zebrafish model was used to assess the toxicity of LC. Finally, a zebrafish transplantation tumor model of EC was grown and treated with LC. Tumors were monitored and harvested to study the expression of NF-κB. Results: The formation of LC was successfully developed with excellent purity and physical properties. In vitro, LC resulted in dose-dependent inhibition of proliferation, induction of apoptosis, and suppression of Ishikawa and HEC-1 cell motility. LC treatment also suppressed the activation and/or expression of NF-κB, caspase-3, and MMP-9. No demonstrable toxicity was found in the zebrafish model and tumors were suppressed after treatment with LC. PCR analysis also showed down-regulated expression of NF-κB. Conclusions: LC was successfully prepared and played biological roles against EC probably through negative regulation of the NF-κB pathway in vitro and in vivo, which demonstrates its potential therapeutic effects in EC.

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“…Moreover, the increment in plasma urea and creatinine levels and the reduction of uric acid in plasma along with histopathological alterations of 2,4-D-treated rats reported by Tayeb et al [8] also supported the impairment of renal functions. Teratogenic [11][12][13], genotoxic [14], neurotoxic [2,[15][16][17], immunosuppressive [18], cytotoxic [19][20], and hepatotoxic [21][22][23] effects of 2,4-D have also been well documented.…”

Section: Introductionmentioning

confidence: 99%

Ameliorative Effects of Melatonin Against 2,4-Dichlorophenoxyacetic Acid Toxicity in Kidney of Mice – A Histological Study.

Upadhyaya

Rao

Jhala

2018

Asian J Pharm Clin Res

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Objective: The compound, 2,4-dichlorophenoxyacetic acid (2,4-D), is a systemic phenoxy herbicide and a plant growth regulator. Histopathological effects of 2,4-D alone and in combination with melatonin on the kidney in adult albino male mice of the Swiss strain were evaluated in the present study.Methods: Twenty adult male albino mice weighing between 30 and 40 g were assigned into four groups with five mice in each group. Group I served as control; Group II -melatonin alone (10 mg/kg body weight); Group III -2,4-D alone (50 mg/kg body weight); and Group IV -2,4-D + melatonin was given orally for 45 days.Results: It revealed a significant reduction in the body and kidney weights (p<0.001 and p<0.01 respectively) of mice treated with 2,4-D, while the body and kidney weights after treatment of melatonin and 2,4-D + melatonin were comparable to control. Histological study revealed that 2,4-D induced nephrotoxicity evidenced by various histological changes including shrinkage of the glomerulus, tubular dilation, hypertrophy of tubular epithelium, and degeneration of renal tubules. Conclusion:Treating animals with the melatonin along with 2,4-D showed mitigative effects of melatonin and minimized the structural alteration in the kidney of mice appeared due to 2,4-D toxicity.

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Developmental toxicity of 2,4-dichlorophenoxyacetic acid in zebrafish embryos

Cited by 125 publications

References 45 publications

Effects of low, subchronic exposure of 2,4‐Dichlorophenoxyacetic acid (2,4‐D) and commercial 2,4‐D formulations on early life stages of fathead minnows (Pimephales promelas)

Effects of low, subchronic exposure of 2,4‐Dichlorophenoxyacetic acid (2,4‐D) and commercial 2,4‐D formulations on early life stages of fathead minnows (Pimephales promelas)

Liposomal Curcumin Targeting Endometrial Cancer Through the NF-κB Pathway

Ameliorative Effects of Melatonin Against 2,4-Dichlorophenoxyacetic Acid Toxicity in Kidney of Mice – A Histological Study.

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