Developmental toxicity and estrogenic potency of zearalenone in zebrafish (Danio rerio)

Bakos, Katalin; Kovács, Róbert; Staszny, Ádám; Sipos, Dóra; Urbányi, Béla; Müller, Ferenc; Csenki, Zsolt; Kovács, Balázs

doi:10.1016/j.aquatox.2013.03.004

Cited by 63 publications

(51 citation statements)

References 46 publications

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“…Any disruption in the HPG axis can adversely affect the reproductive endocrine function (Xu et al, ). The reproductive toxicity of ZEA has been reported in several fish species such as carp ( Cyprinus carpio ) (Sandor & Vanyi, ) Atlantic salmon ( Salmo salar ) (Arukwe, Grotmol, Haugen, Knudsen, & Goksøyr, ), rainbow trout ( Oncorhynchus mykiss ) (Olsen, Meussen‐Elholm, Hongslo, Stenersen, & Tollefsen, ), fathead minnow ( Pimephales promelas ) (Johns et al, ), zebrafish ( Danio rerio ) (Bakos et al, ; Schwartz, Thorpe, Bucheli, Wettstein, & Burkhardt‐Holm, ). ZEA and its metabolites competitively binds with estrogen receptors (ER) resulting in estrogenicity.…”

Section: Introductionmentioning

confidence: 99%

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Muthulakshmi

Fallah

Habibi

et al. 2018

J of Applied Toxicology

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In the present study, we aimed to assess the adverse effects of zearalenone (ZEA) at environmentally relevant concentrations (0.5, 1, 5 and 10 μg l ) on hypothalamic-pituitary-gonadal axis associated reproductive function using zebrafish model. ZEA was exposed to female zebrafish for 21 days to assess growth indices such as condition factor, hepatosomatic index, gonadosomatic index and caspase 3 activity. Further, expression of estrogen receptor (ER) α and CYP19a1b genes in the brain, ERα and vitellogenin (Vtg) genes in the liver and follicle-stimulating hormone receptor, luteinizing hormone receptor, ERα, steroidogenic acute regulatory protein, 3β-hydroxysteroid dehydrogenase (HSD), 17-βHSD and CYP19a1 genes in the ovary were also investigated. Our results showed that there were no significant changes in the condition factor and hepatosomatic index, whereas a significant (P < .05) reduction in the gonadosomatic index, increase in caspase 3 activities and Vtg expression was observed at higher concentration. However, no significant changes were observed at lower treatment levels. Further, we also observed significant (P < .05) upregulation in ERα, Vtg, luteinizing hormone receptor, steroidogenic acute regulatory protein, 3β-HSD, 17β-HSD, CYP19a1 and CYP19a1b genes in treatment groups with higher levels of ZEA. Moreover, in histopathological examination, we observed oocyte atresia and oocyte membrane detachment in ovaries at the highest concentration. In conclusion, the present study revealed the negative impact of ZEA on zebrafish reproductive system by involvement of the hypothalamic-pituitary-gonadal axis-associated reproductive function.

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Section: Introductionmentioning

confidence: 99%

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Muthulakshmi

Fallah

Habibi

et al. 2018

J of Applied Toxicology

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“…In fish, hepatotoxic, immunotoxic and genotoxic effects, effects on reproduction and changes of blood parameters have been described so far (Pietsch et al, , 2015bJohns et al, 2009;Schwartz et al, 2010;Woźny et al, 2012;Bakos et al, 2013;Woźny et al, 2015). Toxicity of ZEN has thereby mostly been assumed to be due to its possible endocrine and developmental impairment since it is a potent phytoestrogen (Bakos et al, 2013;Woźny et al, 2015), but several effects of ZEN on fish cells have also been related to oxidative stress (Pietsch et al, 2014a).…”

Section: Discussionmentioning

confidence: 99%

“…While it has been shown that ZEN does not influence feed conversion and growth rates of fish including carp Döll et al, 2011), effects on the immune system and hematological parameters appear to be a major action of this mycotoxin in carp (Pietsch et al, , 2015b. The estrogenic potential of ZEN was discussed, however studies on this aspect yielded contradictive results depending on the fish species, the life stage, the route of exposure and the exposure dose Johns et al, 2009;Schwartz et al, 2010;Bakos et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Oral uptake of ZEN has been found to cause hepatotoxic, immunotoxic and genotoxic effects in fish (Pietsch et al, , 2015b. Furthermore, haematological and reproductive responses were investigated Johns et al, 2009;Schwartz et al, 2010;Woźny et al, 2012;Bakos et al, 2013). In contrast, metabolic consequences in terms of increased energy expenditure due to ZEN exposure have not yet been shown.…”

Section: Introductionmentioning

confidence: 99%

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Physiological responses of carp (Cyprinus carpio L.) to dietary exposure to zearalenone (ZEN)

Pietsch

Junge

2016

Comparative Biochemistry and Physiology Part C: Toxicology &

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Zearalenone (ZEN) is a frequent contaminant of animal feeds, but systemic effects on fish and possible metabolic costs have not yet been investigated. In order to fill this gap a feeding trial with juvenile carp was conducted. The fish were fed ZEN-contaminated diets at three concentrations (low: 332 μg kg , and high: 797 μg kg −1 final feed, respectively) for four weeks. Possible reversible effects of ZEN were evaluated by feeding an additional group with the mycotoxin for four weeks period and the uncontaminated diet for further two weeks. After that possible ZEN effects on enzyme activities in kidney, spleen, liver and muscle tissue were investigated to get an organism-wide aspect of ZEN effects. Most organs appeared to (over)compensate ZEN effects during the exposure to this mycotoxin, which caused metabolic costs. Oxygen consumption increased in fish treated with the two higher ZEN concentrations via the diet. The differences between the treatments persisted also after the recovery phase of two weeks. Thus, the present study provided evidence of effects of ZEN on carbohydrate metabolism, lipid peroxidation in organs and metabolic oxygen demand. This is the first evidence for increased metabolic costs in a fish species due to exposure to the mycotoxin ZEN.

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“…Additionally, in this article, the usefulness of the RT-PCRD technique (real-time polymerase chain reaction doubling) was assessed to measure the rate of vitellogenin induction as an indirect marker on the toxin effects on zebrafish reproduction. 36 Other fungal metabolites studied include citrinin and patulin, which are produced by several fungi. Cardiotoxicity and nephrotoxicity were the most common damages observed in embryos exposed to these mycotoxins 37,38 (Table 1).…”

Section: Zebrafish and Mycotoxin Studiesmentioning

confidence: 99%

Zebrafish (Danio rerio): A Potential Model for Toxinological Studies

2015

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Zebrafish are an emerging basic biomedical research model that has multiple advantages compared with other research models. Given that biotoxins, such as toxins, poisons, and venoms, represent health hazards to animals and humans, a low-cost biological model that is highly sensitive to biotoxins is useful to understand the damage caused by such agents and to develop biological tests to prevent and reduce the risk of poisoning in potential cases of bioterrorism or food contamination. In this article, a narrative review of the general aspects of zebrafish as a model in basic biomedical research and various studies in the field of toxinology that have used zebrafish as a biological model are presented. This information will provide useful material to beginner students and researchers who are interested in developing toxinological studies with the zebrafish model.

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Developmental toxicity and estrogenic potency of zearalenone in zebrafish (Danio rerio)

Cited by 63 publications

References 46 publications

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Mycotoxin zearalenone induced gonadal impairment and altered gene expression in the hypothalamic–pituitary–gonadal axis of adult female zebrafish (Danio rerio)

Physiological responses of carp (Cyprinus carpio L.) to dietary exposure to zearalenone (ZEN)

Zebrafish (Danio rerio): A Potential Model for Toxinological Studies

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