Developmental study of the distribution of hypoxia-induced factor-1 alpha and microtubule-associated protein 2 in children’s brainstem: Comparison between controls and cases with signs of perinatal hypoxia

Coveñas, Rafael; González-Fuentes, J.; Infante, Eloy Rivas; Lagartos-Donate, M.J.; Cebada‐Sánchez, Sandra; Arroyo-Jiménez, M.M.; Insausti, Ricardo; Marcos, Pilar

doi:10.1016/j.neuroscience.2014.04.018

Cited by 14 publications

(20 citation statements)

References 40 publications

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“…Thus, using an immunohistochemical technique the main aim of this work was to determine for the first time the presence (in fibers and/or cell bodies) and the distribution of Vit C in human brainstems during postnatal development. The results obtained here are compared with recently published data concerning the distribution of MAP-2 and HIF-1␣ in the same cases (Coveñas et al, 2014).…”

Section: Introductionmentioning

confidence: 62%

“…As previously described (Cebada-Sánchez et al, 2014;Coveñas et al, 2014), brains were immediately immersion-fixed in 10% formalin after dissection and then transferred to 4% paraformaldehyde in 0.1 M phosphate buffer-saline (PBS), pH 7.4, following the standardized protocol carried out in our Laboratory (Insausti et al, 2010). Brainstems were dissected out and cryoprotected in increasing sucrose solutions (15-30% in PBS) until they sank.…”

Section: Tissue Processingmentioning

confidence: 99%

“…In this work, the free-floating immunohistochemical procedure was performed following a previously described protocol (CebadaSánchez et al, 2014;Coveñas et al, 2014;Mangas et al, 2009b;Marcos et al, 2011Marcos et al, , 2013 specially adapted to improve immunolabeling sensitivity and minimize background in human tissue (Ramos-Vara, 2005). The protocol applied here has been previously validated for the detection of calcium-binding proteins (Graterón et al, 2003), neuropeptides such as somatostatin and neuropeptide Y (Cebada-Sánchez et al, 2014), and for the mapping of HIF-1␣ and MAP-2 (Coveñas et al, 2014) in the brains of the same cases studied here.…”

Section: Tissue Processingmentioning

confidence: 99%

“…The protocol applied here has been previously validated for the detection of calcium-binding proteins (Graterón et al, 2003), neuropeptides such as somatostatin and neuropeptide Y (Cebada-Sánchez et al, 2014), and for the mapping of HIF-1␣ and MAP-2 (Coveñas et al, 2014) in the brains of the same cases studied here. The highly specific polyclonal primary antibody against Vit C (GEMAC S. A., Saint Jean d'Illac, France; reference: AP-131) was developed in rats and was diluted 1/500, and incubations with Table 1 List of the cases considered for this study.…”

Section: Tissue Processingmentioning

confidence: 99%

See 3 more Smart Citations

Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry

Coveñas

González-Fuentes

Infante

et al. 2015

Annals of Anatomy - Anatomischer Anzeiger

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Section: Introductionmentioning

confidence: 62%

Section: Tissue Processingmentioning

confidence: 99%

Section: Tissue Processingmentioning

confidence: 99%

Section: Tissue Processingmentioning

confidence: 99%

See 2 more Smart Citations

Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry

Coveñas

González-Fuentes

Infante

et al. 2015

Annals of Anatomy - Anatomischer Anzeiger

Self Cite

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“…A study of 16 patients with severe traumatic brain injury found that serum MAP2 concentrations correlated with neurologic outcome at 6 months after injury (Mondello et al, 2012c). Perinatal asphyxia has been shown to affect the distribution of MAP2 in the brainstem of children (Covenas et al, 2014).…”

Section: Microtubule-associated Protein 2 (Map2)mentioning

confidence: 99%

Blood biomarkers for evaluation of perinatal encephalopathy: state of the art

Graham

Everett

Delpech

et al. 2018

Current Opinion in Pediatrics

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Recent research in identification of brain injury after trauma shows many possible blood biomarkers that may help identify the fetus and neonate with encephalopathy. Traumatic brain injury shares many common features with perinatal hypoxic-ischemic encephalopathy. Trauma has a hypoxic component, and one of the 1st physiologic consequences of moderate-severe traumatic brain injury is apnea. Trauma and hypoxia-ischemia initiate an excitotoxic cascade and free radical injury followed by the inflammatory cascade, producing injury in neurons, glial cells and white matter. Increased excitatory amino acids, lipid peroxidation products, and alteration in microRNAs and inflammatory markers are common to both traumatic brain injury and perinatal encephalopathy. The blood-brain barrier is disrupted in both leading to egress of substances normally only found in the central nervous system. Brain exosomes may represent ideal biomarker containers, as RNA and protein transported within the vesicles are protected from enzymatic degradation. Evaluation of fetal or neonatal brain derived exosomes that cross the blood-brain barrier and circulate peripherally has been referred to as the "liquid brain biopsy." A multiplex of serum biomarkers could improve upon the current imprecise methods of identifying fetal and neonatal brain injury such as fetal heart rate abnormalities, meconium, cord gases at delivery, and Apgar scores. Quantitative biomarker measurements of perinatal brain injury and recovery could lead to operative delivery only in the presence of significant fetal risk, triage to appropriate therapy after birth and measure the effectiveness of treatment.

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Investigation of vascular endothelial growth factor receptor‐dependent neuroplasticity on rat nucleus tractus solitarius and phrenic nerve after chronic sustained hypoxia

Kuloğlu

Basaran

Yakan

2021

J Biochem & Molecular Tox

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The neuronal system that controls respiration creates plasticity in response to physiological changes. Chronic sustained hypoxia causes neuroplasticity that contributes to ventilatory acclimatization to hypoxia (VAH). The purpose of this study is to explain the potential roles of the VAH mechanism developing because of chronic sustained hypoxia on respiratory neuroplasticity of vascular endothelial growth factor (VEGF) receptor activation on the nucleus tractus solitarius (NTS) and phrenic nerve. In this study 24 adult male Sprague–Dawley rats were used. Subjects were separated into four groups, a moderate‐sham (mSHAM), severed‐sham (sSHAM), moderate chronic sustained hypoxia (mCSH), and severed chronic sustained hypoxia (sCSH). Normoxic group (mSHAM and sSHAM) rats were exposed to 21% O₂ level (7 days) in the normobaric room while hypoxia group (mCSH and sCSH) rats were exposed to 13% and 10% O₂ level (7 days). Different protocols were applied for normoxic and hypoxia groups and ventilation, respiratory frequency, and tidal volume measurements were made with whole‐body plethysmography. After the test HIF‐1α, erythropoietin (EPO), and VEGFR‐2 expressions on the NTS region in the medulla oblongata and phrenic nerve motor neurons in spinal cord tissue were analyzed using the immunohistochemical stain method. Examinations on the medulla oblongata and spinal cord tissues revealed that HIF‐1α, EPO, and VEGFR‐2 expressions increased in hypoxia groups compared to normoxic groups while a similar increase was also seen when respiratory parameters were assessed. Consequently, learning about VAH‐related neuroplasticity mechanisms developed as a result of chronic continuous hypoxia will contribute to developing new therapeutical approaches to various diseases causing respiratory failure using brain plasticity without recourse to medicines.

show abstract

Developmental study of the distribution of hypoxia-induced factor-1 alpha and microtubule-associated protein 2 in children’s brainstem: Comparison between controls and cases with signs of perinatal hypoxia

Cited by 14 publications

References 40 publications

Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry

Developmental study of vitamin C distribution in children's brainstems by immunohistochemistry

Blood biomarkers for evaluation of perinatal encephalopathy: state of the art

Investigation of vascular endothelial growth factor receptor‐dependent neuroplasticity on rat nucleus tractus solitarius and phrenic nerve after chronic sustained hypoxia

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