Developmental stage and genotype dependent behavioral effects of embryonic alcohol exposure in zebrafish larvae

Abozaid, Amira; Trzuskot, Lidia; Najmi, Zelaikha; Paul, Ishti; Tsang, Benjamin

doi:10.1016/j.pnpbp.2019.109774

Cited by 19 publications

(11 citation statements)

References 46 publications

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“…EAE had no detectable impact on food consumption in adult males, suggesting that it is an unlikely mechanism for increased adiposity ( Figure 4B). Multiple studies have demonstrated that EAE affects zebrafish swimming behaviors (35)(36)(37)(38). To determine whether reduced locomotion was present in adults after EAE, we assessed activity level (35,(39)(40)(41)(42)(43)(44).…”

Section: Resultsmentioning

confidence: 99%

Fetal alcohol spectrum disorder predisposes to metabolic abnormalities in adulthood

Weeks¹,

Bossé²,

Oderberg³

et al. 2020

Journal of Clinical Investigation

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Section: Resultsmentioning

confidence: 99%

Fetal alcohol spectrum disorder predisposes to metabolic abnormalities in adulthood

Weeks¹,

Bossé²,

Oderberg³

et al. 2020

Journal of Clinical Investigation

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“…Here, however, we found a linear dose dependent reduction of distance moved in fish to whom buspirone was administered. The literature, however, does provide some controversies as swimming activity has also been found to increase, rather than decrease, in response to fear inducing stimuli in some zebrafish studies [71,77]. Immobility has also been used to quantify effects of fear and anxiety inducing stimuli or drugs.…”

Section: Discussionmentioning

confidence: 99%

“…Because Big Al's Aquarium obtains their fish from commercial breeding facilities where the number of breeding pairs is large, we assume that the decreased level of inbreeding meets the above criteria. We have also argued that the increased genetic variability and high heterozygosity ratio makes the WT population more representative of species-typical features of zebrafish found in nature [44,71]. Unlike this genetically heterogeneous population, standard zebrafish strains may have unique, strain-specific idiosyncratic features.…”

Section: Animals and Housingmentioning

confidence: 97%

“…Immobility here is defined as fewer than 20% of the number of pixels corresponding to the total visible surface area of the subject changing from one video-frame to the next (with 30 frame per s temporal resolution). Although intuitively total distance travelled and immobility duration may seem to be redundant, negatively correlating measures, empirical data have suggested that they represent independent behavioral responses [41,44,71,75]. Frequency of immobility (number of immobility episodes) was also quantified as it may represent a behavioral strategy independent of the duration of immobility.…”

Section: Experimental Design and Behavioral Testingmentioning

confidence: 99%

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Behavioral Effects of Buspirone in Juvenile Zebrafish of Two Different Genetic Backgrounds

Abozaid

2022

Toxics

Self Cite

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Anxiety continues to represent a major unmet medical need. Despite the availability of numerous anxiolytic drugs, a large proportion of patients do not respond well to current pharmacotherapy, or their response diminishes with chronic drug application. To discover novel compounds and to investigate the mode of action of anxiolytic drugs, animal models have been proposed. The zebrafish is a novel animal model in this research. It is particularly appropriate, as it has evolutionarily conserved features, and drug administration can be employed in a non-invasive manner by immersing the fish into the drug solution. The first step in the analysis of anxiolytic drugs with zebrafish is to test reference compounds. Here, we investigate the effects of buspirone hydrochloride, an anxiolytic drug often employed in the human clinic. We utilize two genetically distinct populations of zebrafish, ABSK, derived from the quasi-inbred AB strain, and WT, a genetically heterogeneous wild-type population. We placed juvenile (10–13-day, post-fertilization, old) zebrafish singly in petri dishes containing one of four buspirone concentrations (0 mg/L control, 5 mg/L, 20 mg/L or 80 mg/L) for 1 h, with each fish receiving a single exposure to one concentration, a between subject experimental design. Subsequently, we recorded the behavior of the zebrafish for 30 min using video-tracking. Buspirone decreased distance moved, number of immobility episodes and thigmotaxis, and it increased immobility duration and turn angle in a quasi-linear dose dependent but genotype independent manner. Although it is unclear whether these changes represent anxiolysis in zebrafish, the results demonstrate that behavioral analysis of juvenile zebrafish may be a sensitive and simple way to quantify the effects of human anxiolytic drugs.

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“…Native from Asia, the zebrafish is a teleost that has high genetic and physiological homology with humans (Lieschke and Currie, 2007), by this reason, this species has been used as a research animal model for different lines such as embryology and development (Hao et al, 2013;Keller et al, 2008), oxidative stress (Choi et al, 2010;Marcon et al, 2018), behavior (Abozaid et al, 2020;Nabinger et al, 2021;Reis et al, 2020) and genetic (Nasevicius and Ekker, 2000;Falcão et al, 2021). Moreover, this aquatic animal is a very interesting environmental bioindicator used in toxicology and ecotoxicology research, due to its capacity to simulate the conditions of an animal in its natural ecosystem (Asharani et al, 2008;Park et al, 2020;Valadas et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Ochratoxin A induces behavioral and neurochemical changes in adult zebrafish

Valadas

Sachett

Marcon

et al. 2021

Preprint

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Ochratoxin A (OTA) is a mycotoxin produced by species of filamentous fungi widely found as a contaminant in food and with high toxic potential. Studies have shown that this toxin cause kidney and liver damage, however, data on the effects of exposure to OTA on the central nervous system are still scarce. Zebrafish (Danio rerio) is a teleost often used in translational research due to its physiological, genetic, and behavioral homology with mammals, in addition to being useful as an environmental bioindicator. Thus, this study aimed to investigate the effects of exposure to OTA on behavioral and neurochemical parameters in adult zebrafish. The animals were treated with different doses of OTA (1.38, 2.77, and 5.53 mg/kg) and submitted to behavioral evaluations in the open tank and social interaction tests. Subsequently, they were euthanized, and the brains were used to assess markers associated with oxidative status. In the open tank test OTA induced changes in distance, absolute turn angle, mean speed, and time-freezing. However, no significant effects were observed in the social interaction test. Moreover, OTA also induced alterations in neurochemical parameters with changes in non-protein thiols (NPSH), glutathione peroxidase (GPx), glutathione-S-transferase (GST), and glutathione reductase (GR). This study showed that OTA can affect neurobiological aspects in zebrafish even at low doses.

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Developmental stage and genotype dependent behavioral effects of embryonic alcohol exposure in zebrafish larvae

Cited by 19 publications

References 46 publications

Fetal alcohol spectrum disorder predisposes to metabolic abnormalities in adulthood

Fetal alcohol spectrum disorder predisposes to metabolic abnormalities in adulthood

Behavioral Effects of Buspirone in Juvenile Zebrafish of Two Different Genetic Backgrounds

Ochratoxin A induces behavioral and neurochemical changes in adult zebrafish

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