Developmental Social Environment Imprints Female Preference for Male Song in Mice

Asaba, Akari; Okabe, Shota; Nagasawa, Miho; Kato, Masahiro; Koshida, Nobuyoshi; Osakada, Takuya; Mogi, Kazutaka; Kikusui, Takefumi

doi:10.1371/journal.pone.0087186

Cited by 66 publications

(122 citation statements)

References 24 publications

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“…A previous study has revealed that females in pro-estrus or estrus do not show the preference for USVs of males of a different strain observed here 14 . Therefore, only females in diestrus were used in the present study; in general, when using females for this test, the phase of the estrous cycle should be known.…”

Section: Discussioncontrasting

confidence: 47%

“…In a previous study, the preference of females for some USVs over others was potentiated when they were exposed to male-soiled bedding, or to the male pheromone ESP1 25 before behavioral testing. In the absence of male-odor stimuli, females did not show a preference for USVs of males of a different strain 14 .…”

Section: Discussionmentioning

confidence: 79%

“…In addition, Figure 4 shows the choice of B6 and BALB females between simultaneous USV playbacks from a BALB and a B6 male, as reported in a previous study 14 . The characteristics of male USVs differ between B6 and BALB strains 20 .…”

Section: Introductionmentioning

confidence: 82%

“…To reveal these mechanisms, the playback experiment is an efficient method. Recent playback studies have revealed that female mice are attracted to USVs 12 , and that they prefer USVs from males that are different from their parents 13,14 . This article explains the playback test used to evaluate USV preference in mice.…”

Section: Introductionmentioning

confidence: 99%

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Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test

Asaba¹,

Kato²,

Koshida³

et al. 2015

JoVE

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Mice emit ultrasonic vocalizations (USVs) during a variety of conditions, such as pup isolation and adult social interactions. These USVs differ with age, sex, condition, and genetic background of the emitting animal. Although many studies have characterized these differences, whether receiver mice can discriminate among objectively different USVs and show preferences for particular sound traits remains to be elucidated. To determine whether mice can discriminate between different characteristics of USVs, a playback experiment was developed recently, in which preference responses of mice to two different USVs could be evaluated in the form of a place preference.First, USVs from mice were recorded. Then, the recorded USVs were edited, trimmed accordingly, and exported as stereophonic sound files. Next, the USV amplitudes generated by the two ultrasound emitters used in the experiment were adjusted to the same sound pressure level. Nanocrystalline silicon thermo-acoustic emitters were used to play the USVs back. Finally, to investigate the preference of subject mice to selected USVs, pairs of two differing USV signals were played back simultaneously in a two-choice test box. By repeatedly entering a defined zone near an ultrasound emitter and searching the wire mesh in front of the emitter, the mouse reveals its preference for one sound over another. This model allows comparing the attractiveness of the various features of mouse USVs, in various contexts. Video LinkThe video component of this article can be found at

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Section: Discussioncontrasting

confidence: 47%

Section: Discussionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test

Asaba¹,

Kato²,

Koshida³

et al. 2015

JoVE

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“…Female urine, but not male urine, also provokes male mice to emit USVs (30). The USVs of male mice attract females and contribute to mate choice by females (31,32). We then examined if this female urine-induced social behavior is altered in ΔD mice.…”

Section: Resultsmentioning

confidence: 99%

Genetic dissection of pheromone processing reveals main olfactory system-mediated social behaviors in mice

Matsuo¹,

Hattori

Asaba

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Most mammals have two major olfactory subsystems: the main olfactory system (MOS) and vomeronasal system (VNS). It is now widely accepted that the range of pheromones that control social behaviors are processed by both the VNS and the MOS. However, the functional contributions of each subsystem in social behavior remain unclear. To genetically dissociate the MOS and VNS functions, we established two conditional knockout mouse lines that led to either loss-of-function in the entire MOS or in the dorsal MOS. Mice with whole-MOS loss-of-function displayed severe defects in active sniffing and poor survival through the neonatal period. In contrast, when loss-of-function was confined to the dorsal MOB, sniffing behavior, pheromone recognition, and VNS activity were maintained. However, defects in a wide spectrum of social behaviors were observed: attraction to female urine and the accompanying ultrasonic vocalizations, chemoinvestigatory preference, aggression, maternal behaviors, and risk-assessment behaviors in response to an alarm pheromone. Functional dissociation of pheromone detection and pheromonal induction of behaviors showed the anterior olfactory nucleus (AON)-regulated social behaviors downstream from the MOS. Lesion analysis and neural activation mapping showed pheromonal activation in multiple amygdaloid and hypothalamic nuclei, important regions for the expression of social behavior, was dependent on MOS and AON functions. Identification of the MOS-AON-mediated pheromone pathway may provide insights into pheromone signaling in animals that do not possess a functional VNS, including humans.social behavior | pheromone processing | main olfactory system | vomeronasal system M ost mammals have two major olfactory subsystems-the main olfactory system (MOS) and vomeronasal system (VNS). The MOS comprises the main olfactory epithelium (MOE), in which olfactory sensory neurons detect odorants, and their projection target, the main olfactory bulb (MOB) (Fig. S1A). Although the MOS is thought to detect volatile odorants and the VNS is thought to be important for the detection of nonvolatile pheromones, evidence shows that the MOS is also involved in pheromone detection (1-8). Surgical blocking of odorant access to the MOE, but not surgical ablation of the vomeronasal epithelium (VNE), eliminates preference to odors from the opposite sex in ferrets (9, 10). In mice, chemical ablation of the MOE impairs male and female sexual behaviors (11,12). In these experiments in which the MOE was ablated, the function of the VNS is not directly disrupted, because the VNS is activated by direct application of urine to the nostril. Thus, these results indicate that the MOS also contributes to pheromone processing and related behaviors.Nonconditional disruption of genes encoding signal transduction proteins that are required for activation of olfactory neurons, such as cyclic nucleotide-gated channel (Cnga2) or adenylyl cyclase 3, impairs several social behaviors (11,(13)(14)(15). However, complete loss of MOS function causes a...

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Testosterone regulates the emission of ultrasonic vocalizations and mounting behavior during different developmental periods in mice

Kikusui

Shima

Sonobe

et al. 2020

Developmental Psychobiology

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Testosterone masculinizes the brain and bodily functions and promotes the development of male characteristics. In the brain, there is a critical period during which testosterone acts on neural circuits to stimulate the development of male-type neural characteristics.Early exposure to testosterone masculinizes reproductive, cognitive, emotional, and neurophysiological functions, such as stress responses (McCarthy & Arnold, 2011). In the developing mammalian brain, testosterone can either be metabolized to estrogen via aromatase, dihydrotestosterone (DHT) via 5α-reductase, or remain unmetabolized. Testosterone and DHT act through a single androgen receptor (AR), while estrogen acts through two types of estrogen receptors (ERα and ERβ) (McCarthy & Arnold, 2011). The relative

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Developmental Social Environment Imprints Female Preference for Male Song in Mice

Cited by 66 publications

References 24 publications

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test

Genetic dissection of pheromone processing reveals main olfactory system-mediated social behaviors in mice

Testosterone regulates the emission of ultrasonic vocalizations and mounting behavior during different developmental periods in mice

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