Developmental Sensitivity of the Bovine Corpus Luteum to Prostaglandin F2α (PGF2α) and Endothelin-1 (ET-1): Is ET-1 a Mediator of the Luteolytic Actions of PGF2α or a Tonic Inhibitor of Progesterone Secretion?1

Choudhary, Ekta; Sen, Aritro; Inskeep, E. K.; Flores, Jorge A.

doi:10.1095/biolreprod.104.034736

Cited by 37 publications

(80 citation statements)

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“…We showed that TNFα increased secretion of PGF2α and ET-1 by LECs in vitro [24]. In fact, several regulatory factors, including cytokines and angiogenic factors, have been shown to regulate not only vascular function (blood flow) but also progesterone production in LSCs [25][26][27][28]. These findings, together with the positive factor VIII staining and the expression of CD-31 mRNA, confirm that the isolated cells consisted mostly of LECs.…”

Section: Discussionsupporting

confidence: 60%

Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production

Acosta

Yoshioka

Komiyama

et al. 2007

Journal of Reproduction and Development

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Abstract. To establish a storage system for isolated bovine luteal endothelial cells (LECs), we investigated the basal and tumor necrosis factor (TNF) α-stimulated production of endothelin-1 (ET-1) and prostaglandin (PG) F2α in unfrozen and frozen-thawed LECs until passage 10. LECs were obtained from developing corpora lutea (CL; days 5-7 of the estrous cycle) using enzymatic digestion and magnetic beads coated with lectin BS-1. The LECs were frozen at -80 C or further cultured and/ or passaged until passage 10 in DMEM/Ham's F-12 supplemented with 10% calf serum. The hormonal productions of unfrozen and frozen/thawed LECs were compared through passages 2-10. When both the unfrozen and frozen/thawed cells reached confluence, the culture medium was replaced with fresh medium containing 0.1% bovine serum albumin (BSA), and the cells were incubated with TNFα (50 ng/ml) for 12 h. The basal productions of ET-1 and PGF2α by the unfrozen and frozen/thawed LECs were similar at passage 2. The basal production of PGF2α by LECs was not altered by passage and storage at -80 C, whereas the basal production of ET-1 decreased from passage 2 and 3 to passage 4 in the unfrozen LECs and from passage 2 to passage 3 in the frozen/thawed LECs. However, production of ET-1 by the unfrozen and frozen/thawed LECs was similar between passages 4-10 and passages 3-10, respectively. Exposure of LECs to TNFα increased (P<0.05) ET-1 and PGF2α production by the unfrozen and frozen-thawed LECs in all passages examined. Thus, LECs obtained from developing CLs and stored until passage 10 can be used for study of the physiology of LECs in vitro. Key words: Bovine, Corpus luteum, Cytokines, Endothelin-1, Endothelium, Prostaglandins (J. Reprod. Dev. 53: [473][474][475][476][477][478][479][480] 2007) he corpus luteum (CL) is a complex organ that mainly consists of endothelial cells (ECs), steroidogenic cells, fibroblasts, and immune cells [1,2]. After ovulation, thecal microvessels invade the granulosal cell layer, and ECs rapidly proliferate in the early CL constituting more than 50% of the total cells in the mid-cycle CL [3][4][5]. The dense vascular network established at mid-cycle is essential for adequate CL function. It also enables an intricate cross-talk between steroidogenic and luteal endothelial cells (LECs) [6]. The products of LECs are directly associated with the capacity of

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Section: Discussionsupporting

confidence: 60%

Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production

Acosta

Yoshioka

Komiyama

et al. 2007

Journal of Reproduction and Development

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“…Throughout the literature lectin binding is recognized as an indication of carbohydrate expression on endothelial cells and as an endothelial cell marker. Lectin binding has been used extensively to isolate microvascular endothelial cells from CL via magnetic bead separation [18][19][20][21][22]. However, the binding properties of lectins, especially BS-1, are also known to be species-, age-, organ-, and vascular bed-specific [23].…”

Section: Discussionmentioning

confidence: 99%

“…One ml of the cell suspension was placed into five 12 × 75 mm polystyrene round bottom test tubes and centrifuged at 250 × g for 10 min. Cell pellets were resuspended and incubated with 100 μl PBS (control) or FITClabeled Bandeiraea simplicifolia (BS-1) and Concanavalin A (ConA) at a concentration of 2.5 μg/ml for 1 h at 4 C. The lectins BS-1 and ConA have been used routinely to isolate endothelial cells and to assess their functional characteristics within the CL [18][19][20][21][22][23], including at least one study in which lectin binding was compared between luteal microvascular endothelial cells of the estrous cycle and pregnancy [24]. Additional controls included samples containing the competing sugar to BS-1 and ConA (200 mM α-D-galactose and α-D-glucose, respectively) [24].…”

Section: Lectin Binding Experimentsmentioning

confidence: 99%

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Microvascular Endothelial Cells of the Bovine Corpus Luteum: A Comparative Examination of the Estrous Cycle and Pregnancy

Cherry

Hou

Rueda

et al. 2008

Journal of Reproduction and Development

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Abstract. Endothelial cells derived from the corpus luteum (CLENDOs) exhibit diverse characteristics presumably serving their wide-ranging roles in luteal function and fate. Here, several attributes of CLENDOs derived from cows at midcycle (days 9-12 of the estrous cycle) were compared with CLENDOs from early pregnancy (day 60 of pregnancy). Flow cytometric analysis of cells fluorescently-tagged with the lectins Bandeiraea simplicifolia (BS-1) and Concanavalin A (ConA) indicated that CLENDOs of midcycle CL do not differ from those of pregnancy. Mean fluorescence intensity for BS-1 was 15 ± 1 and 23 ± 7 fluorescent units for midcycle CLENDOs and CLENDOs of pregnancy, respectively (P>0.05). For ConA, mean fluorescence was 25 ± 2 and 26 ± 1 fluorescent units, respectively (P>0.05). The CLENDOs were also exposed to cytokines to assess differences in activation of nuclear factor kappa B signaling (NF-κB), induction of the transcription factor interferon regulatory factor 1 (IRF1), cytokine production, and cytokine-induced cell death. In response to TNF, for instance, both types of CLENDOs exhibited a rapid, 5-fold decrease in NF-κB inhibitor alpha (NFKBIA) protein expression (P<0.05), and a 4-fold increase in IRF1 expression (P<0.05), that did not differ with phenotype (P>0.05). Similarly, both types of CLENDOs produced tumor necrosis factor alpha and chemokine ligand 2 in response to IFNG stimulation (P<0.05) that did not differ with phenotype (P>0.05). Lastly, extended exposure of CLENDOs of midcycle CL to cytokines induced cell death (~50% cell death vs. control) similar to the incidence of cell death seen previously in CLENDOs of early pregnancy. The results indicate that several physical and functional characteristics of CLENDOs of midcycle CL are retained through early pregnancy, including lectin-binding properties, sensitivity to cytokines, and the activation of cytokine-initiated intracellular signals. Key words: Bovine, Corpus luteum, Cytokine, Endothelial cell, Lectin (J. Reprod. Dev. 54: [183][184][185][186][187][188][189][190][191] 2008) he corpus luteum (CL) is a temporary endocrine gland that forms within the ovary following ovulation and contributes to estrous/menstrual cycle regularity and the establishment of early pregnancy [1]. Aside from its transient nature, an intriguing aspect of the CL is its adaptable lifespan. In domestic livestock, the CL remains functional for as few as 15-18 days to as many as 200-300 days depending upon species and reproductive status of the animal [2]. In the cow, the CL develops, functions, and begins to regress within 17-18 days of ovulation during the estrous cycle, but retains a functional lifespan of more than 200 days during pregnancy [3] . Similarly, luteal lifespan in the ewe ranges from 15-16 days during the estrous cycle to more than 50 days during pregnancy [4]. In fact in most placental mammals the continuation of luteal function associated with pregnancy extends well beyond the period of a single estrous or menstrual cycle and is required for the establi...

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“…The luteal cell culture procedure was modified from previously published protocols [14,15] as follows. The CL tissue utilized for cell dispersion was sliced with a microtome blade, and the slices were diced with a scalpel into ;1-mm pieces, which were transferred to 50-ml tubes, washed 4 times with HBSS, and shaken (200 rpm for 15 min) in a water bath (378C).…”

Section: Isolation and Culture Of Luteal Cellsmentioning

confidence: 99%

Evidence That High Variation in Ovarian Reserves of Healthy Young Adults Has a Negative Impact on the Corpus Luteum and Endometrium During Estrous Cycles in Cattle1

Jimenez-Krassel

Folger

Ireland

et al. 2009

Biology of Reproduction

123

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Low progesterone concentrations and diminished ovarian reserves (total number of healthy oocytes) during reproductive cycles are linked to infertility in single-ovulating species like cattle. However, the extent and mechanisms whereby the inherently high variation in ovarian reserves may negatively affect progesterone production are unknown. Cattle were chosen to address these questions because the size of their ovarian reserves can be predicted based on an antral follicle count (AFC) during follicular waves. The present study determined if progesterone concentrations, differentiation and function of the corpus luteum (CL), and endometrial thickness differed during reproductive cycles of age-matched healthy young adult cattle with low versus high AFC during follicular waves. The results showed that, despite enhanced LH secretion, progesterone concentrations were lower during estrous cycles for animals with low versus high AFC. Animals with low versus high AFC also had a decreased basal, LH-, and 25-hydroxycholesterol-induced capacity of luteal and granulosal cells to produce progesterone, reduced amounts of STAR and mRNAs for STAR and LH receptor in the CL, and no change in endometrial thickness during estrous cycles. Taken together, these results 1) supported the conclusion that high variation in ovarian reserves of young adults is associated with alterations in differentiation and function of the CL and 2) provided insight into the potential factors that may cause suboptimal luteal function (e.g., heightened LH secretion and desensitization of the LH receptor, diminished LH responsiveness, diminished STAR, inherent deficiency in capacity of granulosal cells to undergo luteinization) and infertility (e.g., low progesterone, poor endometrial growth) in individuals with diminished ovarian reserves.

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Developmental Sensitivity of the Bovine Corpus Luteum to Prostaglandin F2α (PGF2α) and Endothelin-1 (ET-1): Is ET-1 a Mediator of the Luteolytic Actions of PGF2α or a Tonic Inhibitor of Progesterone Secretion?1

Cited by 37 publications

References 38 publications

Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production

Effects of Storage and Passage of Bovine Luteal Endothelial Cells on Endothelin-1 and Prostaglandin F2.ALPHA. Production

Microvascular Endothelial Cells of the Bovine Corpus Luteum: A Comparative Examination of the Estrous Cycle and Pregnancy

Evidence That High Variation in Ovarian Reserves of Healthy Young Adults Has a Negative Impact on the Corpus Luteum and Endometrium During Estrous Cycles in Cattle1

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