Developmental regulation of calmodulin-dependent adenylate cyclase activity in an insect endocrine gland.

Meller, Victoria H.; Sakurai, Shunsuke; Gilbert, Lawrence I.

doi:10.1091/mbc.1.10.771

Cited by 29 publications

(4 citation statements)

References 35 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…3). A similar stagespecific dependence of basal ecdysteroid synthesis and release on the presence of extracellular Ca 2+ was reported for the PGs of Manduca (Meller et al, 1990). The rPTTH-stimulated ecdysteroid synthesis was reduced to levels similar to basal release in the absence of Ca 2+ from the incubation medium as shown in Fig.…”

Section: Discussionsupporting

confidence: 83%

Involvement of Calcium, Inositol-1,4,5 Trisphosphate and Diacylglycerol in the Prothoracicotropic Hormone-Stimulated Ecdysteroid Synthesis and Secretion in the Prothoracic Glands of Bombyx mori

Dedos

Fugo

2001

Zoological Science

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 83%

Involvement of Calcium, Inositol-1,4,5 Trisphosphate and Diacylglycerol in the Prothoracicotropic Hormone-Stimulated Ecdysteroid Synthesis and Secretion in the Prothoracic Glands of Bombyx mori

Dedos

Fugo

2001

Zoological Science

View full text Add to dashboard Cite

“…Further work demonstrated that PTTH-stimulated increases in prothoracic gland cAMP generation and ecdysteroid synthesis were dependent upon the influx of external calcium, implicating the participation of a Ca2+-calmodulin dependent adenyl cyclase (Smith et al, 1985;Meller et al, 1988Meller et al, , 1990. The influx of Ca 2+ has recently been shown to occur via L-type Ca 2+ channels (Birkenbeil, 1996;Girgenrath and Smith, 1996) but the mechanism by which PTTH controls the opening of such channels is not known nor is the nature of the PTTH receptor itself (see Gilbert et al, 1996).…”

Section: Early Events In the Ptth Transductory Cascadementioning

confidence: 99%

Control of ecdysteroidogenesis: Activation and inhibition of prothoracic gland activity

Gilbert

Song

Rybczynski

1997

Invertebrate Neuroscience

View full text Add to dashboard Cite

The ecdysteroid hormones, mainly 20-hydroxyecdysone (20E), play a pivotal role in insect development by controlling gene expression involved in molting and metamorphosis. In the model insect Manduca sexta the production of ecdysteroids by the prothoracic gland is acutely controlled by a brain neurohormone, prothoracicotropic hormone (PTTH). PTTH initiates a cascade of events that progresses from the influx of Ca2+ and cAMP generation through phosphorylation of the ribosomal protein S6 and S6-dependent protein synthesis, and concludes with an increase in the synthesis and export of ecdysteroids from the gland. Recent studies indicate that S6 phosphorylation probably controls the steroidogenic effect of PTTH by gating the translation of selected mRNAs whose protein products are required for increased ecdysteroid synthesis. Inhibition of S6 phosphorylation prevents an increase in PTTH-stimulated protein synthesis and subsequent ecdysteroid synthesis. Two of the proteins whose translations are specifically stimulated by PTTH have been identified, one being a beta tubulin and the other a heat shock protein 70 family member. Current data suggest that these two proteins could be involved in supporting microtubule-dependent protein synthesis and ecdysone receptor assembly and/or function. Recent data also indicate that the 20E produced by the prothoracic gland feeds back upon the gland by increasing expression and phosphorylation of a specific USP isoform that is a constituent of the functional ecdysone receptor. Changes in the concentration and composition of the ecdysone receptor complex of the prothoracic gland could modulate the gland's potential for ecdysteroid synthesis (e.g. feedback inhibition) by controlling the levels of enzymes or other proteins in the ecdysteroid biosynthetic pathway.

show abstract

“…Exposure of PGs to neurohormonal factors is also associated with changes in transcriptional and translational activity (Keightley et al, 1990;Rybczynski and Gilbert, 1994), as well as phosphorylation of the ribosomal protein, S6 (Song and Gilbert, 1994). Moreover, the response characteristics of Manduca PGs undergo developmental changes in the final larval instar that may reflect regulatory features important for the normal onset of metamorphosis (Smith and Pasquarello, 1989;Meller et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Comparison of ecdysteroid production in Drosophila and Manduca: Pharmacology and cross‐species neural reactivity

Henrich

1995

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

In both Manduca sexta and Drosophila melanogaster, metamorphic events are driven by ecdysteroids whose production in prothoracic gland (PGs) is stimulated periodically by neural factors. Differences in the life cycle of moths and flies have made it difficult to compare the regulation of ecdysteroid biosynthesis in these two species. As in Manduca, at least two neural factors in the larval Drosophila BVG complex were separable by molecular weight, and they stimulated increased ecdysteroid biosynthesis from the ring gland, a composite organ that includes PG cells. Drosophila neural extracts accelerated ecdysteroid biosynthesis in Manduca PGs and, conversely, partially purified Manduca PTTH preparations elevated ecdysteroid biosynthesis in Drosophila ring glands, suggesting that the two species may share structurally similar prothoracicotropic factors. Drosophila ring glands required the presence of calcium ions to respond to neural extracts, but the phosphodiesterase inhibitor MIX and cAMP analogues exerted little, if any, positive effect on production. Mean ecdysteroid production rates of BVG-ring gland complexes taken from Drosophila larvae during various phases of the wandering period were often submaximal and highly variable, suggesting that they fluctuate widely prior to pupariation. Based on available data in Drosophila and the Manduca model for the control of ecdysteroid biosynthesis, a developmental scheme for neuroendocrine control in Drosophila is proposed.

show abstract

Developmental regulation of calmodulin-dependent adenylate cyclase activity in an insect endocrine gland.

Cited by 29 publications

References 35 publications

Involvement of Calcium, Inositol-1,4,5 Trisphosphate and Diacylglycerol in the Prothoracicotropic Hormone-Stimulated Ecdysteroid Synthesis and Secretion in the Prothoracic Glands of Bombyx mori

Involvement of Calcium, Inositol-1,4,5 Trisphosphate and Diacylglycerol in the Prothoracicotropic Hormone-Stimulated Ecdysteroid Synthesis and Secretion in the Prothoracic Glands of Bombyx mori

Control of ecdysteroidogenesis: Activation and inhibition of prothoracic gland activity

Comparison of ecdysteroid production in Drosophila and Manduca: Pharmacology and cross‐species neural reactivity

Contact Info

Product

Resources

About