Developmental Regulation of BDNF and NT-3 Expression by Quinolinic Acid in the Striatum and Its Main Connections

Checa, Núria; Canals, Josep M.; Alberch, Jordi

doi:10.1006/exnr.2000.7451

Cited by 15 publications

(15 citation statements)

References 58 publications

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“…mRNA BDNF expression was low and reduced with time in agreement with our IHC results. In another study mRNA BDNF showed its maximal level by the third postnatal week (~P21) when the PCR was carried out with the RNAse protection technique 6, 7, hence discrepancies in the expression level obtained in the present study may due to differences in the experimental procedure, still our data are in consonance with those reporting that BDNF presence in the striatal tissue declines with time 3.…”

Section: Discussionsupporting

confidence: 83%

“…In mice striatum, neurotrophin expression is important for the establishment of neuronal connections as well as its correct cell functions. Previous studies have established messenger RNA (mRNA) expression of NGF 4, BDNF 5-7, NT-3 7, 8 and NT-4/5 9 in neostriatal tissue, although these molecules have not always been detected by Northern blot in fetal or postnatal brain 10. In addition to the presence of neurotrophin mRNA, expression of neurotrophin receptors have been documented during the early development of the striatum 3, 7, 8, 11-13.…”

Section: Introductionmentioning

confidence: 99%

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Differential expression of neurotrophins in postnatal C57BL/6 mice striatum

Zermeño¹,

Espíndola²,

Mendoza³

et al. 2009

Int. J. Biol. Sci.

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Neurotrophin expression in early stages of development is crucial for brain assembly and function. In particular, postnatal expression of neurotrophins has not been well documented in the neostriatum and in general neurotrophins or their receptor mRNA's are normally reported, but not protein expression. In the present study, immunocytochemical expression of BDNF, NT-3 and NT-4/5 was characterized in striatal tissue of C57BL/6 mice at postnatal days 10 th (P10), 21 st (P21), 42 nd (P42) and 80 th (P80). We found that the expression of BDNF diminished along the postnatal time course we evaluated, while staining for NT-4 increased up to age P42 and remained constant, thereafter in the cell's soma. In contrast, NT-3 was first expressed in the neostriatal bundles and later on, in neostriatal cell somas. These results provide information about differences in the spatial and temporal expression of each neurotrophin in the neostriatum during the first 80 th postnatal days. RT-PCR procedures were also carried out to further determine whether protein levels of neurotrophins observed in the neostriatum were under control of gene expression. All neurotrophin mRNAs were expressed and only mRNA BDNF was reduced during the postnatal evaluated days. Differences in temporal expression of neurotrophins may be related to the heterochronic development of neostriatal cell populations, but also with the specificity of each neurotrophin modulating different neuronal targets.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Differential expression of neurotrophins in postnatal C57BL/6 mice striatum

Zermeño¹,

Espíndola²,

Mendoza³

et al. 2009

Int. J. Biol. Sci.

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“…We have shown previously that activation of glutamate receptors located on striatal neurons results in a specific pattern of neurotrophin expression during the development (Checa et al, 2000) and in adult excitotoxic models of Huntington's disease (Canals et al, 1998). Combined, all these results indicate that changes in neurotrophin expression may be involved in the selective vulnerability of striatal populations observed in Huntington's disease (Ferrante et al, 1985;Reiner et al, 1988;Richfield et al, 1995).…”

Section: Discussionmentioning

confidence: 78%

“…Although it has been widely demonstrated that synaptic activity regulates neurotrophin expression (Thoenen, 1995;McAllister et al, 1999), it has been suggested that neuronal activity alone is not a sufficient stimulus for the induction of BDNF mRNA after seizures (DugichDjordjevic et al, 1992). In previous studies, we have shown that intrastriatal injection of glutamate receptor agonists induced a differential regulation of the expression of neurotrophins and their receptors (Canals et al, 1998(Canals et al, , 1999Checa et al, 2000). These results could be related to a direct activation of specific glutamate receptors, although injury-induced changes in neurotrophin expression cannot be ruled out.…”

Section: Discussionmentioning

confidence: 86%

“…Our previous results showing that striatal excitotoxic lesions differentially regulated neurotrophin expression in various brain regions (Canals et al, 1998;Checa et al, 2000) suggest that changes in the levels of neurotrophins, including BDNF, could underlie the differential vulnerability of striatal neurons observed in Huntington's disease. The main goal of the present study is to characterize the regulation of BDNF in the cortex of rat models of Huntington's disease.…”

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confidence: 96%

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Expression of Brain-Derived Neurotrophic Factor in Cortical Neurons Is Regulated by Striatal Target Area

et al. 2001

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Changes in BDNF expression after different types of brain insults are related to neuroprotection, stimulation of sprouting, and synaptic reorganization. In the cerebral cortex, an autocrine-paracrine mechanism for BDNF has been proposed because the distribution patterns of BDNF and TrkB expression are almost identical. Moreover, cortical BDNF is anterogradely transported to the striatum, suggesting a role of BDNF in the functional interaction between the two brain regions. Here we have examined the expression of this neurotrophin in the cerebral cortex after various striatal lesions. Intrastriatal injection of quinolinate, kainate, 3-nitropropionic acid, or colchicine increased BDNF mRNA levels in cerebral cortex. In contrast, stimulation of neuronal activity in the striatum did not change cortical BDNF expression. Both excitatory amino acids increased BDNF expression in neurons of cortical layers II/III, V, and VI that project to the striatum. Moreover, grafting a BDNFsecreting cell line prevented both the loss of striatal neurons and the cortical upregulation of BDNF induced by excitotoxins. Because retrograde transport in the corticostriatal pathway was intact after striatal lesions, our results suggest that striatal damage upregulates endogenous BDNF in corticostriatal neurons by a transneuronal mechanism, which may constitute a protective mechanism for striatal and/or cortical cells.

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Glial cell line-derived neurotrophic factor promotes the arborization of cultured striatal neurons through the p42/p44 mitogen-activated protein kinase pathway

et al. 2006

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Glial cell line-derived neurotrophic factor (GDNF) promotes the survival or differentiation of several types of neurons. This study examines GDNF-induced signal transduction and biological effects in cultured striatal neurons. Results show that GDNF addition to striatal cultures transiently increased the protein levels of phosphorylated p42/p44, but did not change the levels of phosphorylated Akt. GDNF effects on phosphorylated p42/p44 levels were blocked by the mitogen-activated protein kinase (MAPK) pathway specific inhibitors (PD98059 and U0126). Activation of the p42/p44 MAPK pathway by GDNF led to an increase in the degree of dendritic arborization and axon length of both GABA- and calbindin-positive neurons but had no effect on their survival and maturation. These GDNF-mediated effects were suppressed in the presence of the inhibitor of the MAPK pathway (PD98059). Furthermore, the addition of the phosphatidylinositol 3-kinase pathway specific inhibitor (LY294002) blocked GDNF-mediated striatal cell differentiation suggesting that the basal activity of this pathway is needed for the effects of GDNF. Our results indicate that treatment of cultured striatal cells with GDNF specifically activates the p42/p44 MAPK pathway, leading to an increase in the arborization of GABA- and calbindin-positive neurons.

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Developmental Regulation of BDNF and NT-3 Expression by Quinolinic Acid in the Striatum and Its Main Connections

Cited by 15 publications

References 58 publications

Differential expression of neurotrophins in postnatal C57BL/6 mice striatum

Differential expression of neurotrophins in postnatal C57BL/6 mice striatum

Expression of Brain-Derived Neurotrophic Factor in Cortical Neurons Is Regulated by Striatal Target Area

Glial cell line-derived neurotrophic factor promotes the arborization of cultured striatal neurons through the p42/p44 mitogen-activated protein kinase pathway

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