Developmental profiles of 5-aminolevulinate, porphobilinogen and porphobilinogen synthase activity in Pieris brassicae related to the synthesis of the bilin-binding protein

Kayser, Hartmut; Krull-Savage, Ute; Gessel, Roland Rilk-van

doi:10.1016/j.ibmb.2004.11.001

Cited by 8 publications

(13 citation statements)

References 29 publications

(42 reference statements)

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“…The ALAD activity is expressed as nmol of PBG formed/ mg protein/h at 37°C. PBG content determination was performed as described (Mauzerall and Granick, 1956;Kayser et al, 2005). Briefly, samples were homogenized and resuspended in 1 mL of 0.15 M cold trichloroacetic acid.…”

Section: Alad Enzyme Activity Determination and Pbg Measurementmentioning

confidence: 99%

Transposase-Derived Proteins FHY3/FAR1 Interact with PHYTOCHROME-INTERACTING FACTOR1 to Regulate Chlorophyll Biosynthesis by Modulating HEMB1 during Deetiolation in Arabidopsis

et al. 2012

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Successful chlorophyll biosynthesis during initial light exposure is critical for plant survival and growth, as excess accumulation of chlorophyll precursors in darkness can cause photooxidative damage to cells. Therefore, efficient mechanisms have evolved to precisely regulate chlorophyll biosynthesis in plants. Here, we identify FAR-RED ELONGATED HYPOCOTYL3 (FHY3) and FAR-RED IMPAIRED RESPONSE1 (FAR1), two transposase-derived transcription factors, as positive regulators of chlorophyll biosynthesis in Arabidopsis thaliana. We show that null mutations in FHY3 and FAR1 cause reduced protochlorophyllide (a precursor of chlorophyll) levels in darkness and less photobleaching in the light. We find that FHY3 directly binds to the promoter and activates expression of HEMB1, which encodes 5-aminolevulinic acid dehydratase in the chlorophyll biosynthetic pathway. We reveal that PHYTOCHROME-INTERACTING FACTOR1 physically interacts with the DNA binding domain of FHY3, thereby partly repressing FHY3/FAR1-activated HEMB1 expression. Strikingly, FHY3 expression is upregulated by white light. In addition, our genetic data indicate that overexpression, severe reduction, or lack of HEMB1 impairs plant growth and development. Together, our findings reveal a crucial role of FHY3/FAR1 in regulating chlorophyll biosynthesis, thus uncovering a new layer of regulation by which light promotes plant dark-light transition in early seedling development.

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Section: Alad Enzyme Activity Determination and Pbg Measurementmentioning

confidence: 99%

Transposase-Derived Proteins FHY3/FAR1 Interact with PHYTOCHROME-INTERACTING FACTOR1 to Regulate Chlorophyll Biosynthesis by Modulating HEMB1 during Deetiolation in Arabidopsis

et al. 2012

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“…4, BBP makes up for the most predominant protein in extracts from both forewings and hind wings. Furthermore, this $20 kDa protein is present at higher concentration in hind wings than in forewings (fourtimes, according to Kayser et al, 2005). The $40 kDa protein that is seen only in extracts from hind wings likely represents actin.…”

Section: Basic Data and Incorporation Of Precursorsmentioning

confidence: 97%

“…The presence of ALA in the wings, though in minute amounts only (as determined in total wings, cf. Kayser et al, 2005), might indicate its local origin. Since, in developing pupae, [ 14 C]ALA is predominantly allocated to BBP bilin, not to the concomitantly synthesized mitochondrial cytochromes in the flight muscles, as we showed in previous work (Kayser and Krull-Savage, 1984), the formation of ALA for the wing bilin most likely takes place in the wings.…”

Section: Article In Pressmentioning

confidence: 99%

Butterfly wings, a new site of porphyrin synthesis and cleavage: Studies on the expression of the lipocalin bilin-binding protein in Pieris brassicae

Sehringer

Kayser

2006

Insect Biochemistry and Molecular Biology

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“…However, lipocalins have been described in other arthropods that are not involved in blood-feeding, but in processes such as development, coloration, defense mechanisms, transport of ligands (Kayser et al 2005;Sánchez et al 1995Sánchez et al , 2000Sánchez et al , 2006Mauchamp et al 2006;Andersen et al 2005;Weichsel et al 1998). The possibility thus exists that lipocalins might play a much larger role in tick biology that is not limited to the feeding process alone.…”

Section: Introductionmentioning

confidence: 99%

Savicalin, a lipocalin from hemocytes of the soft tick, Ornithodoros savignyi

et al. 2010

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Savicalin, is a lipocalin found in the hemocytes of the soft tick, Ornithodoros savignyi. It could be assigned to the tick lipocalin family based on BLAST analysis. Savicalin is the first non-salivary gland lipocalin described in ticks. The mature sequence is composed of 188 amino acids with a molecular mass of 21481.9 Da. A homolog for savicalin was found in a whole body EST-library from a related soft tick O. porcinus, while other tick salivary gland derived lipocalins retrieved from the non-redundant sequence database are more distantly related. Homology modeling supports the inclusion of savicalin into the lipocalin family. The model as well as multiple alignments suggests the presence of five disulphide bonds. Two conserved disulphide bonds are found in hard and soft tick lipocalins. A third disulphide bond is shared with the TSGP4-clade of leukotriene C4 binding soft tick lipocalins and a fourth is shared with a lipocalin from the hard tick Ixodes scapularis. The fifth disulphide bond is unique and links  strands D-E. Phylogenetic analysis showed that savicalin is a distant relative of salivary gland derived lipocalins, but groups within a clade that is possibly non-salivary gland derived. It lacks the biogenic amine-binding motif associated with tick histamine and serotonin binding proteins. Expression profiles indicate that savicalin is found in hemocytes, midgut and ovaries, but not in the salivary glands. Up-regulation occurs in hemocytes after bacterial challenge and in midguts and ovaries after feeding. Given its tissue distribution and upregulation of expression, it is possible that this lipocalin functions in tick development after feeding or in an anti-microbial capacity.

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Developmental profiles of 5-aminolevulinate, porphobilinogen and porphobilinogen synthase activity in Pieris brassicae related to the synthesis of the bilin-binding protein

Cited by 8 publications

References 29 publications

Transposase-Derived Proteins FHY3/FAR1 Interact with PHYTOCHROME-INTERACTING FACTOR1 to Regulate Chlorophyll Biosynthesis by Modulating HEMB1 during Deetiolation in Arabidopsis

Transposase-Derived Proteins FHY3/FAR1 Interact with PHYTOCHROME-INTERACTING FACTOR1 to Regulate Chlorophyll Biosynthesis by Modulating HEMB1 during Deetiolation in Arabidopsis

Butterfly wings, a new site of porphyrin synthesis and cleavage: Studies on the expression of the lipocalin bilin-binding protein in Pieris brassicae

Savicalin, a lipocalin from hemocytes of the soft tick, Ornithodoros savignyi

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