Developmental potential of <i>in vitro</i> or <i>in vivo</i> matured oocytes

Alcoba, Diego Duarte; Pimentel, Ana Laura; Brum, Ilma Simoni; Corleta, Helena von Eye

doi:10.1017/s0967199413000233

Cited by 17 publications

(13 citation statements)

References 30 publications

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“…The rates of usable embryos generated per oocyte (26%) and live-birth oocyte utilisation for GV− patients (5.7%) that we found were similar to rates of 31% and 5% found previously in much larger studies [15]. Furthermore, the proportions of MII and immature oocytes that we found,~85% and 15%, respectively, are also entirely consistent with published literature [7][8][9][10]. Collectively, this supports that although our study group was small, it was a representative population.…”

Section: Discussionsupporting

confidence: 91%

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The Presence of Immature GV− Stage Oocytes during IVF/ICSI Is a Marker of Poor Oocyte Quality: A Pilot Study

et al. 2020

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Here we investigate whether the presence of germinal vesicle-stage oocytes (GV− oocytes) reflects poor oocyte developmental competence (or quality). This was a prospective, non-randomised, cohort pilot-study involving 60 patients undergoing in vitro fertilization/ intracytoplasmic sperm injection for whom complete pregnancy outcome data were available. Patients in whom GV− oocytes were retrieved (GV+) at transvaginal oocyte retrieval (TVOR) were compared with those from whom no GVs were retrieved (GV−). We found that GV+ (n = 29) and GV− (n = 31) patients were similarly aged (35.4 vs. 36.4 years; p = 0.446). GV+ patients had a mean of 2.41 ± 2.03 GVs and comparable yields of MII oocytes to GV− patients (11 ± 6.88 vs. 8.26 ± 4.84; p = 0.077). Compared with GV− patients, GV+ patients had markedly lower implantation rates (11.8% vs. 30.2%; p = 0.022) as well as oocyte utilisation rates for clinical pregnancy (2.3% vs. 6.8%; p = 0.018) and live-birth (1.9% vs. 5.7%; p = 0.029). DNA damage levels measured using γH2AX immunostaining were not different in oocytes from women <36 years versus those ≥36 years (p = 0.606). Thus, patients who have GV− stage oocytes at TVOR exhibit poor oocyte quality reflected in reduced per-oocyte pregnancy success rates and uniformly high levels of oocyte DNA damage.

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Section: Discussionsupporting

confidence: 91%

“…Following hCG trigger during stimulated IVF cycles, typically around 70-85% of oocytes obtained at transvaginal oocyte retrieval (TVOR) are mature (MII-arrested) eggs with the other 15-30% being at MI-and GV− stages [7][8][9][10]. It is not known why some oocytes remain GV− arrested despite being exposed to an hCG stimulus.…”

Section: Introductionmentioning

confidence: 99%

The Presence of Immature GV− Stage Oocytes during IVF/ICSI Is a Marker of Poor Oocyte Quality: A Pilot Study

et al. 2020

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“…We found that an MI oocyte was 7.6 times more likely to have fertilization failure than an MII oocyte. A low normal fertilization rate of immature oocytes has been reported by other studies, and among MI oocytes, the fertilization rate was lower than that of in vitro-matured MI oocytes (Alcoba et al, 2015;De Vincentiis et al, 2013;Strassburger et al, 2004;Shu et al, 2007). The results of this study indicate that oocyte maturation has a direct influence on fertilization rates.…”

Section: Discussionsupporting

confidence: 45%

“…To be fertilized, oocytes should ideally reach maturity, as oocytes in PI and MI are too morphologically immature to be fertilized for the formation of good-quality embryos. MI-stage oocytes can spontaneously undergo in vitro meiotic maturation to MII-stage oocytes within a few hours after collection (Strassburger et al, 2004), but they generally remain unused because their rates of fertilization, blastocyst formation and implantation are lower than those of MII oocytes that mature in vivo (Strassburger et al, 2004;Alcoba et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

The usefulness of metaphase I oocytes in women who undergo controlled ovarian hyperstimulation for intracytoplasmic sperm injection

Bilibio

Lorenzzoni

Meireles

et al. 2020

JBRA Assisted Reproduction

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Objective: The aim of this study was to evaluate the fertilization and blastocyst formation rates of oocytes in metaphase I (MI) obtained from women who underwent controlled ovarian hyperstimulation (COH) for intracytoplasmic injection. Methods: A prospective cohort study that included women from whom at least 1 MI and 1 MII oocyte were obtained after COH was performed. We collected 1,907 oocytes from 164 women (1291 MII, 352 MI and 258 prophase I or atretic). After oocyte classification, the MII and MI oocytes were incubated for 4 hours. Results: After 4 hours, the rescue maturation rate was 57.2%; 205 MI oocytes matured to MII oocytes in vitro (rescued MI-MII group), and 153 remained in MI (arrested MI group). The normal fertilization rates were directly associated with oocyte maturation, with rates of 79.1%, 60.2%, and 31.9% in MII, MI-MII and MI oocytes, respectively (p<0.001). Group arrested MI had an odds ratio (OR) of 7.6 (CI 5.2-11.2, p<0.001) for abnormal fertilization compared with Group MII. The blastocyst formation rate was directly associated with oocyte maturation, at 36.4% for MII, 11.4% for MI-MII and 0.6% for MI. Conclusion: Oocytes collected at the MI stage after OCH that did not mature to MII after rescue maturation had a blastocyst formation rate of only 0.6%, while those in MII and MI-MII had rates of 36.4% and 11.4%, respectively. However, we found a pregnancy with the birth of a healthy baby from a blastocyst formed after intracytoplasmic sperm injection (ICSI) of an MI oocyte.

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“…, 2010 ; Vieira et al. , 2011 ; Alcoba et al. , 2015 ) or on the use of IVM with intention-to-treat patients ( Bos-Mikich et al , 2011 ; Lucena & Moreno-Ortiz, 2013 ; Tominaga et al , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Occurrence of ovarian follicular dominance during stimulation for IVM impacts usable blastocyst yield

Romero¹,

Pella²,

Escudero³

et al. 2018

JBRA Assisted Reproduction

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ObjectiveTo evaluate the influence of ovarian follicular dominance on the outcome of oocyte in-vitro maturation.MethodsThis retrospective cohort study included 21 patients with polycystic ovaries or polycystic ovary syndrome (Rotterdam criteria, 2004) subjected to 24 in-vitro maturation (IVM) cycles between October 2015 and January 2017. Patients undergoing IVM received minimal gonadotropin stimulation starting on day 2 or 3 of the cycle; ovum pick-up typically occurred on days 6 to 8. No hCG-trigger shot was given. Following 30h of IVM, mature oocytes were inseminated by ICSI and the resulting embryos cultured up to the blastocyst stage.ResultsOvarian follicular dominance was observed in nine of the 24 IVM cycles. Oocyte IVM yielded an overall maturation rate of 69.3±23.8%, and no difference was observed when the groups with or without a dominant follicle were assessed independently. The rates of fertilization and usable blastocysts per fertilized oocyte, mature oocyte (Metaphase II) or cumulus-oocyte-complex were nearly three times higher (28.7±22.5%) in the group without ovarian follicular dominance. No differences were found in the clinical pregnancy rates attained by the individuals with or without a dominant follicle after 21 vitrified-warmed blastocyst transfer cycles.ConclusionOccurrence of ovarian follicular dominance during hormonal stimulation for in-vitro maturation negatively impacted embryological outcomes. Strategies devised to limit the appearance of ovarian follicular dominance must be further explored.

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Developmental potential of in vitro or in vivo matured oocytes

Cited by 17 publications

References 30 publications

The Presence of Immature GV− Stage Oocytes during IVF/ICSI Is a Marker of Poor Oocyte Quality: A Pilot Study

The Presence of Immature GV− Stage Oocytes during IVF/ICSI Is a Marker of Poor Oocyte Quality: A Pilot Study

The usefulness of metaphase I oocytes in women who undergo controlled ovarian hyperstimulation for intracytoplasmic sperm injection

Occurrence of ovarian follicular dominance during stimulation for IVM impacts usable blastocyst yield

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