Developmental plasticity of Arabidopsis hypocotyl is dependent on exocyst complex function

Drdová, Edita Janková; Klejchová, Martina; Janko, Karel; Hála, Michal; Soukupová, Hana; Cvrčková, Fatima; Žárský, Viktor

doi:10.1093/jxb/erz005

Cited by 15 publications

(9 citation statements)

References 38 publications

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“…The multiplicity of the plant exocyst complex functions has been well documented. Distinct exocyst variants are involved in auxin transport, root and hypocotyl epidermal cell elongation, cytokinesis, pollen tube growth, seed coat formation, defense against pathogens, xylem differentiation, and leaf trichome development ( Cole et al., 2005 ; Synek et al., 2006 ; Hála et al., 2008 ; Kulich et al., 2015 ; Sekereš et al., 2017 ; Vukašinović et al., 2017 ; Janková Drdová et al., 2019 ). On the subcellular level, exocyst participates in plasma membrane protein recycling, cytokinesis, autophagic targeting to the vacuole, and deposition of cell wall material, especially callose, to the pathogen attack site ( Fendrych et al., 2010 ; Pecenková et al., 2011 ; Drdová et al., 2013 ; Žárský et al., 2013 ; Kulich et al., 2018 ; rev.…”

Section: Introductionmentioning

confidence: 99%

Redundant and Diversified Roles Among Selected Arabidopsis thaliana EXO70 Paralogs During Biotic Stress Responses

et al. 2020

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The heterooctameric vesicle-tethering complex exocyst is important for plant development, growth, and immunity. Multiple paralogs exist for most subunits of this complex; especially the membrane-interacting subunit EXO70 underwent extensive amplification in land plants, suggesting functional specialization. Despite this specialization, most Arabidopsis exo70 mutants are viable and free of developmental defects, probably as a consequence of redundancy among isoforms. Our in silico datamining and modeling analysis, corroborated by transcriptomic experiments, pinpointed several EXO70 paralogs to be involved in plant biotic interactions. We therefore tested corresponding single and selected double mutant combinations (for paralogs EXO70A1, B1, B2, H1, E1, and F1) in their two biologically distinct responses to Pseudomonas syringae, root hair growth stimulation and general plant susceptibility. A shift in defense responses toward either increased or decreased sensitivity was found in several double mutants compared to wild type plants or corresponding single mutants, strongly indicating both additive and compensatory effects of exo70 mutations. In addition, our experiments confirm the lipid-binding capacity of selected EXO70s, however, without the clear relatedness to predicted C-terminal lipid-binding motifs. Our analysis uncovers that there is less of functional redundancy among isoforms than we could suppose from whole sequence phylogeny and that even paralogs with overlapping expression pattern and similar membrane-binding capacity appear to have exclusive roles in plant development and biotic interactions.

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Section: Introductionmentioning

confidence: 99%

Redundant and Diversified Roles Among Selected Arabidopsis thaliana EXO70 Paralogs During Biotic Stress Responses

et al. 2020

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“…Phenotypic plasticity usually refers to the adaptability of an individual (or a genotype) to cope with environmental changes by modulating one or multiple phenotypic traits (Arnold et al ., 2019; Fusco and Minelli, 2010). The genetic and environmental impacts on the regulation of gene expression or physiological traits were extensively studied in several plant species and specific tissues or organs (Gruber et al ., 2013; dal Santo et al ., 2016; Dal Santo et al ., 2013; Janková Drdová et al ., 2019; Jong et al ., 2019; Ibañez et al ., 2017). Conversely, little if any information is available on the impact of the environment on the induction or repression of the specialized metabolome, especially in seeds.…”

Section: Discussionmentioning

confidence: 99%

Untargeted metabolomic analyses reveal the diversity and plasticity of the specialized metabolome in seeds of differentCamelina sativagenotypes

Boutet

Barreda

Perreau

et al. 2021

Preprint

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SummarySeeds produce a myriad of Specialized Metabolites (SMs). Nevertheless, despite the essential role of SMs in the interaction of plants with their environment, studying the ability of crop seeds to produce these protective compounds has been neglected. Camelina is an oilseed crop, whose seeds are characterized by high oil content and a unique composition, including broad SM diversity. We characterized SM landscapes in the seeds of six camelina cultivars grown in the open field and harvested during five consecutive growing seasons. We achieved a comprehensive annotation of camelina seed SMs combining molecular and correlation network analyses, which cluster SMs based on their chemical structures and co-accumulation patterns, respectively. Thus, we were able to evaluate the impact of the genotype and environment on the accumulation of these metabolites. Our data showed surprisingly high and unexplored effects of the environment on the stimulation of the seed-specialized metabolome. Moreover, they emphasize that seed SMs display a much higher environmental plasticity than storage compounds (e.g. oil and proteins). Last, we identified flavonols as the most plastic metabolic class, revealing highly variable accumulation according to the environmental conditions and/or the genotype. This work highlights the predominant effect of the environment on the regulation of the seed-specialized metabolome, with a potential impact on the seed quality of camelina and other crop species.Significance statementSeeds produce a myriad of Specialized Metabolites (SMs) with an essential role in the interaction of plants with their environment. We characterized SM landscapes in the seeds of six camelina cultivars grown in the open field and harvested during five consecutive growing seasons. We highlight the predominant effect of the environment on the regulation of the seed-specialized metabolome, with a potential impact on seed quality of camelina and other crop plant species.

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“…The study showed that the members of EXO70A, EXO70C, and EXO70G isoforms are usually linked with the growth and development of plants ( Lai, 2016 ; Synek et al, 2017 ; Du et al, 2018 ). Arabidopsis EXO70A1, the best characterized members of EXO70A isoform, involved in cytokinesis ( Fendrych et al, 2010 ), root hair and cell growth ( Synek et al, 2006 ; Wu et al, 2013 ; Cole et al, 2014 ), pollen–stigma interactions ( Safavian et al, 2015 ), hypocotyl development ( Hala et al, 2008 ; Jankova Drdova et al, 2019 ), and primary and secondary cell wall biogenesis ( Li et al, 2013 ; Oda et al, 2015 ; Vukasinovic et al, 2017 ). Instead, EXO70B, E, F, and H isoforms are often related to plant biotic interactions and defense responses.…”

Section: Discussionmentioning

confidence: 99%

The Exocyst Complex Subunit EXO70E1-V From Haynaldia villosa Interacts With Wheat Powdery Mildew Resistance Gene CMPG1-V

Zhao

Zhang

et al. 2021

Front. Plant Sci.

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EXO70 belongs to the exocyst complex subunit that plays a critical role in regulating plant cell polarity establishment and defense response. A previous study proved that the E3 ligase CMPG1-V from Haynaldia villosa, a diploid wheat relative, positively regulates the resistance to wheat powdery mildew (Pm), caused by fungus Blumeria graminis f.sp tritici (Bgt). In this study, a member of EXO70 superfamily named EXO70E1-V was isolated from H. villosa, and EXO70E1-V interacted with CMPG1-V were shown by yeast two-hybrid (Y2H), pull-down assay, bimolecular fluorescence complementation (BiFC) assay, and luciferase complementation imaging (LCI) assay. It is localized in various subcellular organs, i.e., plasma membrane (PM) and endoplasmic reticulum. Co-expression of EXO70E1-V and CMPG1-V showed dot-like structure fluorescence signals that were mainly in PM and nucleus. Expression of EXO70E1-V was relatively higher in leaf and was significantly induced by Bgt infection and exogenous application of hormones such as salicylic acid. Transient or stable overexpression of EXO70E1-V could not enhance/decrease the Pm resistance level, suggesting overexpression of EXO70E1-V alone has no impact on Pm resistance in wheat.

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Developmental plasticity of Arabidopsis hypocotyl is dependent on exocyst complex function

Abstract: Etiolated hypocotyls of exocyst mutants exhibit a defect in hypocotyl plasticity manifested as ectopic collet-like structure formation, associated with deviations in auxin signaling and starch accumulation.

Cited by 15 publications

References 38 publications

Redundant and Diversified Roles Among Selected Arabidopsis thaliana EXO70 Paralogs During Biotic Stress Responses

Redundant and Diversified Roles Among Selected Arabidopsis thaliana EXO70 Paralogs During Biotic Stress Responses

Untargeted metabolomic analyses reveal the diversity and plasticity of the specialized metabolome in seeds of differentCamelina sativagenotypes

The Exocyst Complex Subunit EXO70E1-V From Haynaldia villosa Interacts With Wheat Powdery Mildew Resistance Gene CMPG1-V

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