Developmental Physiology

Fellenberg, Günter

doi:10.1007/978-3-662-12810-7_12

Cited by 5 publications

(4 citation statements)

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“…Chromatin was purified by filtration through Sephadex G-25 Fine using 1M NaCl buffer. The purity of chromatin preparations was checked using the criteria of Fellenberg, (1974).…”

Section: -Purification Of Chromatinmentioning

confidence: 99%

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Physico–Chemical Properties of Chromatin and Induction of Ploidy in Sugarcane

2012

Alexandria Science Exchange Journal: An International Quarterly

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This work was carried out at Subbhia experimental station for sugar crops. The present investigation aims at inducing polyploidy in sugar cane (Saccharum officinarum) and to investigate its agronomic traits. Colchicine was used to produce poly ploidy from three cultivars. These cultivars are (C9 G.T. 54-9, PH 8013 and NCO 310). Buds were treated with 0.05% colchicine solution. Treated segments were allowed to germinate until a shoot system length of 40 cm had reached. Plants were then transferred and allowed to grow in the yield. The growing plants were examined for polyploidy firstly by Guard cells of Stomata, Chloroplasts, DNA content in leaves, and chromosomes from adventitious roots initiated at the laboratory. Physico-chemical properties for chromatins and deproteinized DNA were estimated. Total soluble solids (T.S.S); plant height and plant diameter were estimated. The obtained results would be summarized as follows: The present studies aimed to produce polyploidy in cultivar C9. The obtained results showed that the average of polyploidy was found to be 9% for C9 and 4% for PH 8013. Regarding chemical composition and melting temperatures of chromatins different ratios were obtained reflecting differential transcriptional activities. However, active and repressed fractions of chromatin were estimated. With respect to the evaluated agronomic characteristics different values were observed.

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“…Chromatin was purified by filtration through Sephadex G-25 Fine using 1M NaCl buffer. The purity of chromatin preparations was checked using the criteria of Fellenberg, (1974).…”

Section: -Purification Of Chromatinmentioning

confidence: 99%

“…In order to estimate the thermal stability of sugar cane chromatin (against temperature) as well as the chemical composition, the following procedure was used: -Chromatin isolation was done by the method described by Fellenberg andSchömer, (1975), andSeehy et al, (1984).…”

Section: -Estimation Of Thermal Stability and Chemical Compositionmentioning

confidence: 99%

Physico–Chemical Properties of Chromatin and Induction of Ploidy in Sugarcane

2012

Alexandria Science Exchange Journal: An International Quarterly

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“…On peut se rallier a ! 'interpretation selon laquelle un grandissement important est favorise par un cycle cellulaire au cours duquella mitose est occultee ce qui empeche une chute de Ia synthese d' ARN (Fellenberg, 1974 ). L' endopolyploldisation des cellules serait done, chez certaines especes.…”

Section: -Deuxieme Etape : Phase Pre-accumulatriceunclassified

“…On enregistre, en outre, une Iegere augmentation de Ia valeur 2C d' ADN ainsi que de Ia valeur 4C, augmentation qui disparait lorsque s' achevent Ia croissance et I' accumulation (Tort et al, 1990). Cette amplification de I' ADN, accroissement tres inferieur a Ia duplication, est habituellement interpretee comme le resultat de copies reiterees un tres grand nombre de fois de quelques genes (Fellenberg, 1974 ). Une de ses caracteristiques est Ia degradation des genes surexprimes une fois le codage termine (Nagl, 1979).…”

Section: -Deuxieme Etape : Phase Pre-accumulatriceunclassified