Developmental Pattern, Tissue Distribution, and Subcellular Distribution of Cysteine: α-Ketoglutarate Aminotransferase and 3-Mercaptopyruvate Sulfurtransferase Activities in the Rat

Abstract

The tissue distribution, the subcellular distribution in liver, and the developmental patterns of cysteine:α-ketoglutarate aminotransferase (CAT) and 3-mercaptopyruvate sulfurtransferase (MPST) activities were determined in rats of the Sprague-Dawley strain. CAT activity was highest in heart and liver, whereas MPST activity was highest in liver and kidney. CAT activity was located primarily in the mitochondrial fraction with a low level of activity in all other fractions. MPST activity was located in both the mitochondrial and cytosolic fractions. The specific activity of CAT and MPST generally increased in rat liver, heart, and kidney during the developmental period from 18 days of gestation to 26 days after birth. Peak CAT-specific activity occurred at about 4 days of age and peak MPST activity occurred at about 21 days of age; peak activity of both CAT and MPST exceeded the adult specific activities. The distribution and developmental pattern of hydrogen sulfide production from cysteine, catalyzed by the coupled activities of CAT and MPST, was similar to those of CAT activity.

Sulfotransferase in Humans: Development and Tissue Distribution

“…Sulfotransferase activity (pmol X m in"1 X mg') indifferent human fetal tissues in rats and the developmental pattern is substrate-dependent. Chen et al [1982] de scribed the postnatal development of sulfo transferase towards bile salts and Kuo et al [1983] studied the developmental pattern of sulfotransferase towards 3-mcrcaptopyruvate: cyanide in rat liver, kidneys and heart. The presence of the enzyme in the mid-gesta tional human fetus has been described by different authors using varying substrate [Namkung et al, 1976;Rollins el al., 1979;Steiner et al, 1982], The average activity in the fetal liver is about one third of that in the adult speci men.…”

Changes in Cysteine Dioxygenase and Cysteinesulfinate Decarboxylase Activities and Taurine Levels in Tissues of Pregnant or Lactating Rat Dams and Their Fetuses or Pups

“…The day pups were found was counted as day 1 of lactation or day 1 from birth. The procedures fol lowed in mating and housing the dams and their pups have been described previously [32], Litter size was not adjusted and ranged from 4 to 14 pups with a mean ± S D of9.2 ± 2.3.…”

Physicochemical and kinetic characteristics of rhodanese from the liver of African catfish Clarias gariepinus Burchell in Asejire lake

“…However, the identification of metal ions in our study may have arisen from the isolation procedure employed, possibly from the inconclusive separation of the mitochondrial from the cytosolic fraction due primarily to the limitations of our centrifugation procedures. Both rhodanese and 3-MST are localized in both mitochondria and the cytosol of animals, plants and microorganisms (Cosby and Summer 1945;Himwich and Saunders 1948;Sorbo 1951;Jarabak and Westley 1974;Taniguichi and Kimura 1974;Koj et al 1975;Anosike and Ugochukwu 1981;Kuo et al 1983;Lee et al 1995;Vazquez et al 1997;Nagahara et al 1999;Agboola and Okonji 2004). Another possibility is that it may have arisen from the preparative procedures employed in a way similar to the bovine rhodanese in which the predominance of A or B depends on the mildness of the purification medium (Blumenthal and Heinrikson 1971).…”