Developmental Pattern of Thy-1 Immunoreactivity in the Human Kidney and the Application to Pediatric Renal Neoplasms

Abstract

The localization of Thy-1, a surface membrane lipoglycoprotein, was investigated using a monoclonal antibody specific for human Thy-1 (HB-2S-1). The localization of Thy-1 during development was established in a series of five fetal, three childhood, and two adult normal kidneys. In this series, Thy-1 immunolocalization progressed from mesangial and endothelial cell staining in the 16- to 17-week fetuses to similar staining along with staining of the parietal epithelium of the capsule and proximal tubule staining in the 20- to 24-week fetuses. Glomerular mesangial cell and endothelial cell staining was absent by 9 months postnatally when the adult pattern of staining was apparent. The localization of Thy-1 during development was also compared with a series of pediatric renal tumors including 14 Wilms' tumors, 3 congenital mesoblastic nephromas, 1 clear cell sarcoma, and 1 pediatric renal cell carcinoma. Thy-1 staining was demonstrated in epithelial tubules of Wilms' tumors and in the spindle-shaped cells of congenital mesoblastic nephroma correlating with Thy-1 immunoreactivity in the kidney proximal tubule and fetal medullary stroma, respectively. Thy-1 staining was absent in the anaplastic epithelial Wilms' tumor, the renal cell carcinoma, and the clear cell sarcoma. This staining pattern fails to provide evidence that these tumors may arise from the medullary mesenchyme or the differentiated proximal convoluted tubule. These results show that Thy-1 is a renal differentiation marker and is useful in the characterization of tumors of renal development.

Thy-1 Expressing Mesenchymal Cells in Rat Nephrogenesis in Correlation with Cells Immunoreactive for .ALPHA.-Smooth Muscle Actin and Vimentin

“…These findings indicated that mesangial cells started to be formed from the S-shaped body in conjunction with Thy-1 expression; because podocyte folding and vascularization began to be seen in the S-shaped body stage, formation of mesangial cells may be related to podocytes and endothelial cells 23 . Here, we must take into consideration the difference in immunoreactivity of Thy-1 between rats and humans; human Thy-1 expression in mesangial cells is lost with renal development 24 . The expression patterns of mesangial cells for vimentin were similar to those for Thy-1 (Table 1), indicating the mesenchymal nature of the mesangial cells.…”

Parathyroid hormone‐related protein is a hypertrophy factor for human mesangial cells: Implications for diabetic nephropathy

“…PTHrP immunostaining was carried out using rabbit polyclonal C‐terminal PTHrP antiserum C6 (at 1:100 dilution; Izquierdo et al, 2006). To identify HMC, a rabbit polyclonal Thy‐1 antibody (Santa Cruz Biotechnology, Santa Cruz, CA), diluted 1:100, and a mouse α‐smooth muscle actin antibody (Sigma–Aldrich, St. Louis, MO), diluted 1:1,000, were used due to its relative specificity (Alpers et al, 1992; Bukovsky et al, 1992; Hazen‐Martin et al, 1993; Groma et al, 1997; Kuhara et al, 1997). Following incubation with the primary antibody, the sections were incubated with biotinylated anti‐rabbit IgG (Pierce, Rockford, IL), followed by incubation with the avidin‐biotin‐peroxidase complex (Dako, Glostrup, Denmark).…”

All-trans-Retinoic Acid Inhibits the Development of Mesangial Proliferative Glomerulonephritis in Interleukin-6 Transgenic Mice

“…The glomeruli were cultured in D -Val-MEM supplemented with 20% fetal calf serum (Gibco Laboratories, Grand Island, N.Y., USA) and antibiotics (100 U/ml penicillin and 100 µg/ml streptomycin) at 37°C in a 5% CO 2 atmosphere to obtain mesangial cells for the experiments. The isolated rat mesangial cells used for the in vitro experiments were identified by their Thy-1 antigen expression [19], and mesangial cells from the seventh passage were used for our study.…”