Developmental origin and maintenance of distinct testicular macrophage populations

Mossadegh-Keller, Noushine; Gentek, Rebecca; Gimenez, Grégory; Bigot, Sylvain; Mailfert, Sébastien; Sieweke, Michael H.

doi:10.1084/jem.20170829

Cited by 121 publications

(169 citation statements)

References 40 publications

Supporting

Mentioning

160

Contrasting

Order By: Relevance

“…Peritubular macrophages, adjacent to the PTC, originate from BM HSC after birth. 38 In summary, both embryo-and blood monocyte-derived macrophages can give rise to macrophage populations in tissues. 26,39 Within tissues, local microenvironment regulates macrophage turnover, as well as the cellular identity and function of resident phagocytes.…”

Section: Macrophage Biologymentioning

confidence: 98%

“…However, the advent of multicolor flow cytometric analyses and RNA‐Seq‐based genome wide transcription profiling, revealed a much wider range of tissue‐specific phenotypic markers, characterized by unique transcriptional signatures. Mouse TM can be identified by the high expression of F4/80 and CD11b in the absence of Ly6c and CD11c . Mouse TM also express tissue‐resident markers, such as CX3C chemokine receptor 1 (CX3CR1), CD64, M‐CSF receptor 1, and MER proto‐oncogene tyrosine kinase (MerTK).…”

Section: Phenotype Of Tmmentioning

confidence: 99%

“…The 2 TM populations, interstitial TM and peritubular TM, can be distinguished by their distinct surface marker patterns and unique gene expression profiles. Interstitial TM are identified by the following pattern: MCSFR + CD64 + , and MHC II − , whereas peritubular TM are characterized by MCSFR − , CD64 − , and MHC II + . Moreover, peritubular TM are negative for MerTK, which indicates reduced phagocytic function.…”

Section: Phenotype Of Tmmentioning

confidence: 99%

“…Although peritubular TM lack classical macrophage markers, such as CD64 and MerTK, they can be distinguished from conventional DC based on their lack of DC markers, such as fms‐related tyrosine kinase 3 ( Flt3 ), Cd11c , Cd103 , and zinc finger and btb domain containing 46 (Zbtb46 ). However, the genes involved in Ag presentation and processing, namely H2eb1 , H2.k1 , NLR family pyrin domain containing 3 ( Nlrp3 ), and intercellular adhesion molecule 1 ( Icam1 ), are all found highly expressed in peritubular TM …”

Section: Phenotype Of Tmmentioning

confidence: 99%

“…Although peritubular and interstitial TM differ in their regulation of archetypical testicular functions (spermatogenesis and steroidogenesis, respectively), both possibly contribute to the establishment of testicular immune privilege, by displaying an immunoregulatory M2 macrophage phenotype . In this regard, TM are characterized by the expression of a low number of proinflammatory genes, with concomitant high expression levels of a large number of M2 macrophage phenotype genes, such as CD163 …”

Section: Phenotype Of Tmmentioning

confidence: 99%

See 4 more Smart Citations

Microenvironmental signals govern the cellular identity of testicular macrophages

Meinhardt

Wang

Schulz³

et al. 2018

Journal of Leukocyte Biology

View full text Add to dashboard Cite

Testicular macrophages (TM) comprise the largest immune cell population in the mammalian testis. They are characterized by a subdued proinflammatory response upon adequate stimulation, and a polarization toward the immunoregulatory and immunotolerant M2 phenotype. This enables them to play a relevant role in supporting the archetypical functions of the testis, namely spermatogenesis and steroidogenesis. During infection, the characteristic blunted immune response of TM reflects the need for a delicate balance between a sufficiently strong reaction to counteract invading pathogens, and the prevention of excessive proinflammatory cytokine levels with the potential to disturb or destroy spermatogenesis. Local microenvironmental factors that determine the special phenotype of TM have just begun to be unraveled, and are discussed in this review.

show abstract

Section: Macrophage Biologymentioning

confidence: 98%

Section: Phenotype Of Tmmentioning

confidence: 99%

Section: Phenotype Of Tmmentioning

confidence: 99%

Section: Phenotype Of Tmmentioning

confidence: 99%

Section: Phenotype Of Tmmentioning

confidence: 99%

See 3 more Smart Citations

Microenvironmental signals govern the cellular identity of testicular macrophages

Meinhardt

Wang

Schulz³

et al. 2018

Journal of Leukocyte Biology

View full text Add to dashboard Cite

show abstract

Phagocyte Development

Patel

Yona

2018

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Mature circulating white blood cells can be divided into two lineages: lymphoid and myeloid. The lymphoid lineage consists of T, B, NK and innate lymphoid cells, while the myeloid lineage includes functionally and morphologically distinct cell types including mononuclear phagocytes (monocytes, dendritic cells and macrophages), granulocytes (neutrophils, basophils, mast cells and eosinophils) and platelets. Granulocytes and mononuclear phagocytes are key players at all stages of the immune response from its genesis to its resolution, and come under the banner of phagocytes. Damaged or infected tissue releases chemoattractants that rapidly recruit these phagocytes to the site of injury. Once at the inflamed site, these cells coordinate and carry out immune functions, playing a key role in host defence. The recent past has seen major progress in our understanding of phagocyte developmental biology. Here, we discuss the latest advances in myeloid development, underpinning our current understanding of how these cells are generated and maintained. Key Concepts The myeloid lineage includes functionally and morphologically distinct cell types including mononuclear phagocytes (monocytes, dendritic cells and macrophages) and granulocytes (neutrophils, basophils, mast cells and eosinophils). The majority of tissue resident macrophages are generated before birth and are long‐lived self‐maintained cells throughout adulthood. Dendritic cells prime naïve T cells and are derived from a common precursor in the bone marrow that gives rise exclusively to this family of cells. Monocytes are the principal circulating mononuclear phagocyte. Monocytes develop in the bone marrow from a common monocyte progenitor, once in the circulation classical monocytes have the potential to convert into nonclassical monocytes. Classical monocytes are known to repopulate resident mononuclear phagocyte populations and have potent effector functions during immunity. Granulocyte subsets have a complex morphology with a segmented nucleus and each subset contains granules endowing them with a specific role in host defence. Transcription and growth factors control and dictate the development of phagocytes.

show abstract

Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases

Belkacem

Mossadegh-Keller

Bourgoin³

et al. 2021

Advanced Science

View full text Add to dashboard Cite

Blood cell analysis is a major pillar of biomedical research and healthcare. These analyses are performed in central laboratories. Rapid shipment from collection site to the central laboratories is currently needed because cells and biomarkers degrade rapidly. The dried blood spot from a fingerstick allows the preservation of cellular molecules for months but entire cells are never recovered. Here leucocyte elution is optimized from dried blood spots. Flow cytometry and mRNA expression profiling are used to analyze the recovered cells. 50-70% of the leucocytes that are dried on a polyester solid support via elution after shaking the support with buffer are recovered. While red blood cells lyse upon drying, it is found that the majority of leucocytes are preserved. Leucocytes have an altered structure that is improved by adding fixative in the elution buffer. Leucocytes are permeabilized, allowing an easy staining of all cellular compartments. Common immunophenotyping and mRNAs are preserved. The ability of a new biomarker (CD169) to discriminate between patients with and without Severe Acute Respiratory Syndrome induced by Coronavirus 2 (SARS-CoV-2) infections is also preserved.Leucocytes from blood can be dried, shipped, and/or stored for at least 1 month, then recovered for a wide variety of analyses, potentially facilitating biomedical applications worldwide.

show abstract

Developmental origin and maintenance of distinct testicular macrophage populations

Cited by 121 publications

References 40 publications

Microenvironmental signals govern the cellular identity of testicular macrophages

Microenvironmental signals govern the cellular identity of testicular macrophages

Phagocyte Development

Cell Analysis from Dried Blood Spots: New Opportunities in Immunology, Hematology, and Infectious Diseases

Contact Info

Product

Resources

About